Investigating the Antidepressant Mechanisms of <i>Polygonum sibiricum</i> Polysaccharides via Microglial Polarization
<i>Polygonum sibiricum</i>, with its medicinal and edibility dual properties, has been widely recognized and utilized throughout Chinese history. As a kind of its effective component, <i>Polygonum sibiricum</i> polysaccharides (PSP) have been reported to be a promising novel...
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MDPI AG
2024-02-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/16/3/438 |
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| author | Yingyu Zhang Danyang Wang Jiameng Liu Jing Sun Xinmin Liu Bei Fan Cong Lu Fengzhong Wang |
| author_facet | Yingyu Zhang Danyang Wang Jiameng Liu Jing Sun Xinmin Liu Bei Fan Cong Lu Fengzhong Wang |
| author_sort | Yingyu Zhang |
| collection | DOAJ |
| description | <i>Polygonum sibiricum</i>, with its medicinal and edibility dual properties, has been widely recognized and utilized throughout Chinese history. As a kind of its effective component, <i>Polygonum sibiricum</i> polysaccharides (PSP) have been reported to be a promising novel antidepressant agent. Meanwhile, the precise mechanisms underlying its action remain elusive. The polarization state transition of microglia is intricately linked to neuroinflammation, indicating its crucial involvement in the pathophysiology of depression. Researchers are vigorously pursuing the exploration of this potential treatment strategy, aiming to comprehend its underlying mechanisms. Hence, the current study was designed to investigate the antidepressant mechanisms of PSP via Microglial M1/M2 Polarization, based on the lipopolysaccharide (LPS)-induced BV2 cell activation model. The results indicate that PSP significantly inhibited NO and LDH release and reduced ROS levels in LPS-induced BV2 cells. PSP could significantly reduce the protein expression level of Iba-1, decreased the mRNA levels of TNF-α, IL-1β, and IL-6, and increased the mRNA level of IL-10. PSP also significantly reduced the protein expression level of CD16/32 and increased that of CD206, reduced the mRNA level and fluorescence intensity of iNOS, and increased those of Arg-1. However, PSP pretreatment reversed the alterations of the BDNF/TrkB/CREB and Notch/Hes1 pathways in LPS-induced BV2 cells. These results suggested that PSP exerted the anti-inflammatory effects by inhibiting M1 phenotype polarization and promoting microglia polarization toward the M2 phenotype, and its regulation of microglia M1/M2 polarization may be associated with modulating the BDNF/TrkB/CREB and Notch/Hes1 pathways. |
| first_indexed | 2024-03-08T03:50:38Z |
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| language | English |
| last_indexed | 2024-03-08T03:50:38Z |
| publishDate | 2024-02-01 |
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| series | Nutrients |
| spelling | doaj.art-55eb7264eb6b4e33953b6b6d3efc68892024-02-09T15:19:59ZengMDPI AGNutrients2072-66432024-02-0116343810.3390/nu16030438Investigating the Antidepressant Mechanisms of <i>Polygonum sibiricum</i> Polysaccharides via Microglial PolarizationYingyu Zhang0Danyang Wang1Jiameng Liu2Jing Sun3Xinmin Liu4Bei Fan5Cong Lu6Fengzhong Wang7Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, ChinaInstitute of Food Science and Technology, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, ChinaInstitute of Food Science and Technology, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, ChinaInstitute of Food Science and Technology, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, ChinaInstitute of New Drug Technology, Ningbo University, Ningbo 315211, ChinaInstitute of Food Science and Technology, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, ChinaInstitute of Food Science and Technology, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, ChinaInstitute of Food Science and Technology, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, China<i>Polygonum sibiricum</i>, with its medicinal and edibility dual properties, has been widely recognized and utilized throughout Chinese history. As a kind of its effective component, <i>Polygonum sibiricum</i> polysaccharides (PSP) have been reported to be a promising novel antidepressant agent. Meanwhile, the precise mechanisms underlying its action remain elusive. The polarization state transition of microglia is intricately linked to neuroinflammation, indicating its crucial involvement in the pathophysiology of depression. Researchers are vigorously pursuing the exploration of this potential treatment strategy, aiming to comprehend its underlying mechanisms. Hence, the current study was designed to investigate the antidepressant mechanisms of PSP via Microglial M1/M2 Polarization, based on the lipopolysaccharide (LPS)-induced BV2 cell activation model. The results indicate that PSP significantly inhibited NO and LDH release and reduced ROS levels in LPS-induced BV2 cells. PSP could significantly reduce the protein expression level of Iba-1, decreased the mRNA levels of TNF-α, IL-1β, and IL-6, and increased the mRNA level of IL-10. PSP also significantly reduced the protein expression level of CD16/32 and increased that of CD206, reduced the mRNA level and fluorescence intensity of iNOS, and increased those of Arg-1. However, PSP pretreatment reversed the alterations of the BDNF/TrkB/CREB and Notch/Hes1 pathways in LPS-induced BV2 cells. These results suggested that PSP exerted the anti-inflammatory effects by inhibiting M1 phenotype polarization and promoting microglia polarization toward the M2 phenotype, and its regulation of microglia M1/M2 polarization may be associated with modulating the BDNF/TrkB/CREB and Notch/Hes1 pathways.https://www.mdpi.com/2072-6643/16/3/438<i>Polygonum sibiricum</i> polysaccharidesneuroinflammationmicroglia polarization |
| spellingShingle | Yingyu Zhang Danyang Wang Jiameng Liu Jing Sun Xinmin Liu Bei Fan Cong Lu Fengzhong Wang Investigating the Antidepressant Mechanisms of <i>Polygonum sibiricum</i> Polysaccharides via Microglial Polarization Nutrients <i>Polygonum sibiricum</i> polysaccharides neuroinflammation microglia polarization |
| title | Investigating the Antidepressant Mechanisms of <i>Polygonum sibiricum</i> Polysaccharides via Microglial Polarization |
| title_full | Investigating the Antidepressant Mechanisms of <i>Polygonum sibiricum</i> Polysaccharides via Microglial Polarization |
| title_fullStr | Investigating the Antidepressant Mechanisms of <i>Polygonum sibiricum</i> Polysaccharides via Microglial Polarization |
| title_full_unstemmed | Investigating the Antidepressant Mechanisms of <i>Polygonum sibiricum</i> Polysaccharides via Microglial Polarization |
| title_short | Investigating the Antidepressant Mechanisms of <i>Polygonum sibiricum</i> Polysaccharides via Microglial Polarization |
| title_sort | investigating the antidepressant mechanisms of i polygonum sibiricum i polysaccharides via microglial polarization |
| topic | <i>Polygonum sibiricum</i> polysaccharides neuroinflammation microglia polarization |
| url | https://www.mdpi.com/2072-6643/16/3/438 |
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