Unravelling the Complex Interplay of Transcription Factors Orchestrating Seed Oil Content in <i>Brassica napus</i> L.
Transcription factors (TFs) and their complex interplay are essential for directing specific genetic programs, such as responses to environmental stresses, tissue development, or cell differentiation by regulating gene expression. Knowledge regarding TF–TF cooperations could be promising in gaining...
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| Format: | Article |
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MDPI AG
2021-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/3/1033 |
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| author | Abirami Rajavel Selina Klees Johanna-Sophie Schlüter Hendrik Bertram Kun Lu Armin Otto Schmitt Mehmet Gültas |
| author_facet | Abirami Rajavel Selina Klees Johanna-Sophie Schlüter Hendrik Bertram Kun Lu Armin Otto Schmitt Mehmet Gültas |
| author_sort | Abirami Rajavel |
| collection | DOAJ |
| description | Transcription factors (TFs) and their complex interplay are essential for directing specific genetic programs, such as responses to environmental stresses, tissue development, or cell differentiation by regulating gene expression. Knowledge regarding TF–TF cooperations could be promising in gaining insight into the developmental switches between the cultivars of <i>Brassica napus</i> L., namely Zhongshuang11 (ZS11), a double-low accession with high-oil- content, and Zhongyou821 (ZY821), a double-high accession with low-oil-content. In this regard, we analysed a time series RNA-seq data set of seed tissue from both of the cultivars by mainly focusing on the monotonically expressed genes (MEGs). The consideration of the MEGs enables the capturing of multi-stage progression processes that are orchestrated by the cooperative TFs and, thus, facilitates the understanding of the molecular mechanisms determining seed oil content. Our findings show that TF families, such as NAC, MYB, DOF, GATA, and HD-ZIP are highly involved in the seed developmental process. Particularly, their preferential partner choices as well as changes in their gene expression profiles seem to be strongly associated with the differentiation of the oil content between the two cultivars. These findings are essential in enhancing our understanding of the genetic programs in both cultivars and developing novel hypotheses for further experimental studies. |
| first_indexed | 2024-03-09T04:06:30Z |
| format | Article |
| id | doaj.art-55eda0caede1448bbc2b70303582ed52 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T04:06:30Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-55eda0caede1448bbc2b70303582ed522023-12-03T14:04:52ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-01-01223103310.3390/ijms22031033Unravelling the Complex Interplay of Transcription Factors Orchestrating Seed Oil Content in <i>Brassica napus</i> L.Abirami Rajavel0Selina Klees1Johanna-Sophie Schlüter2Hendrik Bertram3Kun Lu4Armin Otto Schmitt5Mehmet Gültas6Breeding Informatics Group, Department of Animal Sciences, Georg-August University, Margarethe von Wrangell-Weg 7, 37075 Göttingen, GermanyBreeding Informatics Group, Department of Animal Sciences, Georg-August University, Margarethe von Wrangell-Weg 7, 37075 Göttingen, GermanyBreeding Informatics Group, Department of Animal Sciences, Georg-August University, Margarethe von Wrangell-Weg 7, 37075 Göttingen, GermanyBreeding Informatics Group, Department of Animal Sciences, Georg-August University, Margarethe von Wrangell-Weg 7, 37075 Göttingen, GermanyCollege of Agronomy and Biotechnology, Southwest University, Beibei, Chongqing 400715, ChinaBreeding Informatics Group, Department of Animal Sciences, Georg-August University, Margarethe von Wrangell-Weg 7, 37075 Göttingen, GermanyBreeding Informatics Group, Department of Animal Sciences, Georg-August University, Margarethe von Wrangell-Weg 7, 37075 Göttingen, GermanyTranscription factors (TFs) and their complex interplay are essential for directing specific genetic programs, such as responses to environmental stresses, tissue development, or cell differentiation by regulating gene expression. Knowledge regarding TF–TF cooperations could be promising in gaining insight into the developmental switches between the cultivars of <i>Brassica napus</i> L., namely Zhongshuang11 (ZS11), a double-low accession with high-oil- content, and Zhongyou821 (ZY821), a double-high accession with low-oil-content. In this regard, we analysed a time series RNA-seq data set of seed tissue from both of the cultivars by mainly focusing on the monotonically expressed genes (MEGs). The consideration of the MEGs enables the capturing of multi-stage progression processes that are orchestrated by the cooperative TFs and, thus, facilitates the understanding of the molecular mechanisms determining seed oil content. Our findings show that TF families, such as NAC, MYB, DOF, GATA, and HD-ZIP are highly involved in the seed developmental process. Particularly, their preferential partner choices as well as changes in their gene expression profiles seem to be strongly associated with the differentiation of the oil content between the two cultivars. These findings are essential in enhancing our understanding of the genetic programs in both cultivars and developing novel hypotheses for further experimental studies.https://www.mdpi.com/1422-0067/22/3/1033<i>Brassica napus</i>MEGsseed oil contentoil synthesistranscription factor cooperation |
| spellingShingle | Abirami Rajavel Selina Klees Johanna-Sophie Schlüter Hendrik Bertram Kun Lu Armin Otto Schmitt Mehmet Gültas Unravelling the Complex Interplay of Transcription Factors Orchestrating Seed Oil Content in <i>Brassica napus</i> L. International Journal of Molecular Sciences <i>Brassica napus</i> MEGs seed oil content oil synthesis transcription factor cooperation |
| title | Unravelling the Complex Interplay of Transcription Factors Orchestrating Seed Oil Content in <i>Brassica napus</i> L. |
| title_full | Unravelling the Complex Interplay of Transcription Factors Orchestrating Seed Oil Content in <i>Brassica napus</i> L. |
| title_fullStr | Unravelling the Complex Interplay of Transcription Factors Orchestrating Seed Oil Content in <i>Brassica napus</i> L. |
| title_full_unstemmed | Unravelling the Complex Interplay of Transcription Factors Orchestrating Seed Oil Content in <i>Brassica napus</i> L. |
| title_short | Unravelling the Complex Interplay of Transcription Factors Orchestrating Seed Oil Content in <i>Brassica napus</i> L. |
| title_sort | unravelling the complex interplay of transcription factors orchestrating seed oil content in i brassica napus i l |
| topic | <i>Brassica napus</i> MEGs seed oil content oil synthesis transcription factor cooperation |
| url | https://www.mdpi.com/1422-0067/22/3/1033 |
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