Prevalence of human papillomavirus in eyelid carcinoma among Koreans: a clinicopathological study
Abstract Background Human papillomavirus (HPV) has been detected in eyelid sebaceous gland carcinoma (SGC) and squamous cell carcinoma (SCC), and detection rates greatly varied across studies. This study aimed to investigate the presence of HPV in eyelid SGC and SCC among Koreans and its correlation...
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| Format: | Article |
| Language: | English |
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BMC
2023-09-01
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| Series: | BMC Ophthalmology |
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| Online Access: | https://doi.org/10.1186/s12886-023-03131-9 |
| _version_ | 1797453473383448576 |
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| author | Min Kyu Yang Namju Kim Hokyung Choung Ji Eun Kim Sang In Khwarg |
| author_facet | Min Kyu Yang Namju Kim Hokyung Choung Ji Eun Kim Sang In Khwarg |
| author_sort | Min Kyu Yang |
| collection | DOAJ |
| description | Abstract Background Human papillomavirus (HPV) has been detected in eyelid sebaceous gland carcinoma (SGC) and squamous cell carcinoma (SCC), and detection rates greatly varied across studies. This study aimed to investigate the presence of HPV in eyelid SGC and SCC among Koreans and its correlation with clinicopathological characteristics. Methods Surgically resected eyelid samples diagnosed as SGC or SCC from January 1999 to June 2011 were identified from the pathology database of three referral centres in Korea. Clinicopathological information including origin (skin vs. tarsal conjunctiva) and treatment outcomes were retrospectively reviewed. Immunohistochemistry (IHC) for p16, HPV DNA in situ hybridisation (ISH), and polymerase chain reaction-based DNA microarray were performed in paraffin-embedded tissue sections. Results Our cohort included 34 SGC and 12 SCC cases with Asian ethnicity. HPV was detected in 4 SGC and 6 SCC by DNA microarray, while 2 SCC (16.7%) showed positivity in ISH. SCC of tarsal conjunctival origin was significantly more common in HPV-positive SCC than in HPV-negative SCC (5 of 6 vs. 0 of 6, P = 0.015, Fisher’s exact test). Among samples showing positive staining in p16 IHC, HPV positivity rates were 0.0% (0/19) in SGC and 100% (3/3) in SCC. There was no significant difference in overall and local recurrence rate in eyelid SGC and SCC according to the HPV status (P > 0.99). Conclusions HPV was found in a subset of eyelid SGC and SCC among Koreans and might be aetiologically related to SCC of tarsal conjunctival origin. Overexpression of p16 is considered to be inappropriate as an indicator of HPV infection in eyelid SGC. Further investigation is required to elucidate the transmission route and pathogenic roles of HPV. |
| first_indexed | 2024-03-09T15:23:20Z |
| format | Article |
| id | doaj.art-55ee655b9f20443c88fe8df01f5f8e46 |
| institution | Directory Open Access Journal |
| issn | 1471-2415 |
| language | English |
| last_indexed | 2024-03-09T15:23:20Z |
| publishDate | 2023-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Ophthalmology |
| spelling | doaj.art-55ee655b9f20443c88fe8df01f5f8e462023-11-26T12:40:13ZengBMCBMC Ophthalmology1471-24152023-09-012311810.1186/s12886-023-03131-9Prevalence of human papillomavirus in eyelid carcinoma among Koreans: a clinicopathological studyMin Kyu Yang0Namju Kim1Hokyung Choung2Ji Eun Kim3Sang In Khwarg4Department of Ophthalmology, Asan Medical CenterDepartment of Ophthalmology, Seoul National University Bundang HospitalDepartment of Ophthalmology, Seoul Metropolitan Government–Seoul National University Boramae Medical CenterDepartment of Pathology, Seoul Metropolitan Government–Seoul National University Boramae Medical CenterDepartment of Ophthalmology, Seoul National University HospitalAbstract Background Human papillomavirus (HPV) has been detected in eyelid sebaceous gland carcinoma (SGC) and squamous cell carcinoma (SCC), and detection rates greatly varied across studies. This study aimed to investigate the presence of HPV in eyelid SGC and SCC among Koreans and its correlation with clinicopathological characteristics. Methods Surgically resected eyelid samples diagnosed as SGC or SCC from January 1999 to June 2011 were identified from the pathology database of three referral centres in Korea. Clinicopathological information including origin (skin vs. tarsal conjunctiva) and treatment outcomes were retrospectively reviewed. Immunohistochemistry (IHC) for p16, HPV DNA in situ hybridisation (ISH), and polymerase chain reaction-based DNA microarray were performed in paraffin-embedded tissue sections. Results Our cohort included 34 SGC and 12 SCC cases with Asian ethnicity. HPV was detected in 4 SGC and 6 SCC by DNA microarray, while 2 SCC (16.7%) showed positivity in ISH. SCC of tarsal conjunctival origin was significantly more common in HPV-positive SCC than in HPV-negative SCC (5 of 6 vs. 0 of 6, P = 0.015, Fisher’s exact test). Among samples showing positive staining in p16 IHC, HPV positivity rates were 0.0% (0/19) in SGC and 100% (3/3) in SCC. There was no significant difference in overall and local recurrence rate in eyelid SGC and SCC according to the HPV status (P > 0.99). Conclusions HPV was found in a subset of eyelid SGC and SCC among Koreans and might be aetiologically related to SCC of tarsal conjunctival origin. Overexpression of p16 is considered to be inappropriate as an indicator of HPV infection in eyelid SGC. Further investigation is required to elucidate the transmission route and pathogenic roles of HPV.https://doi.org/10.1186/s12886-023-03131-9DNA microarrayEyelidHuman papillomavirusSebaceous gland carcinomaSquamous cell carcinoma |
| spellingShingle | Min Kyu Yang Namju Kim Hokyung Choung Ji Eun Kim Sang In Khwarg Prevalence of human papillomavirus in eyelid carcinoma among Koreans: a clinicopathological study BMC Ophthalmology DNA microarray Eyelid Human papillomavirus Sebaceous gland carcinoma Squamous cell carcinoma |
| title | Prevalence of human papillomavirus in eyelid carcinoma among Koreans: a clinicopathological study |
| title_full | Prevalence of human papillomavirus in eyelid carcinoma among Koreans: a clinicopathological study |
| title_fullStr | Prevalence of human papillomavirus in eyelid carcinoma among Koreans: a clinicopathological study |
| title_full_unstemmed | Prevalence of human papillomavirus in eyelid carcinoma among Koreans: a clinicopathological study |
| title_short | Prevalence of human papillomavirus in eyelid carcinoma among Koreans: a clinicopathological study |
| title_sort | prevalence of human papillomavirus in eyelid carcinoma among koreans a clinicopathological study |
| topic | DNA microarray Eyelid Human papillomavirus Sebaceous gland carcinoma Squamous cell carcinoma |
| url | https://doi.org/10.1186/s12886-023-03131-9 |
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