Assessment of Treg-related lncRNAs in epilepsy
Recent studies have shown dysregulation of several groups of long non-coding RNAs in the context of epilepsy. According to evidence regarding the role of regulatory T cells in this disorder, we examined expression levels of regulatory T cell-related lncRNAs, namely TH2-LCR, RMRP, IFNG-AS1 (NEST), MA...
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Molecular Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnmol.2022.1031314/full |
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| author | Guive Sharifi Reyhane Eghtedarian Mohammad Taheri Mohammad Taheri Bashdar Mahmud Hussen Bashdar Mahmud Hussen Solat Eslami Solat Eslami Soudeh Ghafouri-Fard Arezou Sayad |
| author_facet | Guive Sharifi Reyhane Eghtedarian Mohammad Taheri Mohammad Taheri Bashdar Mahmud Hussen Bashdar Mahmud Hussen Solat Eslami Solat Eslami Soudeh Ghafouri-Fard Arezou Sayad |
| author_sort | Guive Sharifi |
| collection | DOAJ |
| description | Recent studies have shown dysregulation of several groups of long non-coding RNAs in the context of epilepsy. According to evidence regarding the role of regulatory T cells in this disorder, we examined expression levels of regulatory T cell-related lncRNAs, namely TH2-LCR, RMRP, IFNG-AS1 (NEST), MAFTRR and FLICR in the blood of epileptic cases compared with controls. Expression of RMRP was lower in patients with refractory epilepsy compared with controls [expression ratio (95% CI) = 0.32 (0.13–0.8), adjusted p-value = 0.0008]. Besides, its expression was lower in refractory patients vs. non-refractory patients [expression ratio (95% CI) = 0.2 (0.1–0.41), adjusted p-value < 0.0001]. Expression of TH2-LCR was lower in refractory patients vs. controls [expression ratio (95% CI) = 0.4 (0.17–0.93), adjusted p-value = 0.0044] and in refractory patients vs. non-refractory ones [Expression ratio = 0.28 (0.19–0.58), p-value < 0.0001]. Expression of NEST was higher in total patients [expression ratio (95% CI) = 2.48 (1.15–5.27), adjusted p-value = 0.0012] and in both groups of patients compared with controls. However, its expression was not different between refractory and non-refractory cases. Similarly, FLICR and MAFTRR were over-expressed in total cases and both groups of patients compared with controls, but their expressions were similar between refractory and non-refractory cases. MAFTRR could differentiate between total epileptic cases and controls with AUC value of 0.8. This lncRNA could separate refractory and non-refractory cases from healthy controls with AUC values of 0.73 and 0.88, respectively. This study provides evidence for deregulation of regulatory T cell-related lncRNAs in epilepsy and their potential role as diagnostic markers in this condition. |
| first_indexed | 2024-04-10T20:18:48Z |
| format | Article |
| id | doaj.art-55eed2c6bf5447f69eaa5dc8012cc0ad |
| institution | Directory Open Access Journal |
| issn | 1662-5099 |
| language | English |
| last_indexed | 2024-04-10T20:18:48Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Molecular Neuroscience |
| spelling | doaj.art-55eed2c6bf5447f69eaa5dc8012cc0ad2023-01-26T04:44:09ZengFrontiers Media S.A.Frontiers in Molecular Neuroscience1662-50992023-01-011510.3389/fnmol.2022.10313141031314Assessment of Treg-related lncRNAs in epilepsyGuive Sharifi0Reyhane Eghtedarian1Mohammad Taheri2Mohammad Taheri3Bashdar Mahmud Hussen4Bashdar Mahmud Hussen5Solat Eslami6Solat Eslami7Soudeh Ghafouri-Fard8Arezou Sayad9Skull Base Research Center, Loghman Hakim Hospital, Shahid Beheshti University of Medical Sciences, Tehran, IranPhytochemistry Research Center, Shahid Beheshti University of Sciences, Tehran, IranUrology and Nephrology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, IranInstitute of Human Genetics, Jena University Hospital, Jena, GermanyDepartment of Pharmacognosy, College of Pharmacy, Hawler Medical University, Erbil, IraqCenter of Research and Strategic Studies, Lebanese French University, Erbil, IraqDepartment of Medical Biotechnology, School of Medicine, Alborz University of Medical Sciences, Karaj, IranDietary Supplements and Probiotic Research Center, Alborz University of Medical Sciences, Karaj, IranMen's Health and Reproductive Health Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran0Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranRecent studies have shown dysregulation of several groups of long non-coding RNAs in the context of epilepsy. According to evidence regarding the role of regulatory T cells in this disorder, we examined expression levels of regulatory T cell-related lncRNAs, namely TH2-LCR, RMRP, IFNG-AS1 (NEST), MAFTRR and FLICR in the blood of epileptic cases compared with controls. Expression of RMRP was lower in patients with refractory epilepsy compared with controls [expression ratio (95% CI) = 0.32 (0.13–0.8), adjusted p-value = 0.0008]. Besides, its expression was lower in refractory patients vs. non-refractory patients [expression ratio (95% CI) = 0.2 (0.1–0.41), adjusted p-value < 0.0001]. Expression of TH2-LCR was lower in refractory patients vs. controls [expression ratio (95% CI) = 0.4 (0.17–0.93), adjusted p-value = 0.0044] and in refractory patients vs. non-refractory ones [Expression ratio = 0.28 (0.19–0.58), p-value < 0.0001]. Expression of NEST was higher in total patients [expression ratio (95% CI) = 2.48 (1.15–5.27), adjusted p-value = 0.0012] and in both groups of patients compared with controls. However, its expression was not different between refractory and non-refractory cases. Similarly, FLICR and MAFTRR were over-expressed in total cases and both groups of patients compared with controls, but their expressions were similar between refractory and non-refractory cases. MAFTRR could differentiate between total epileptic cases and controls with AUC value of 0.8. This lncRNA could separate refractory and non-refractory cases from healthy controls with AUC values of 0.73 and 0.88, respectively. This study provides evidence for deregulation of regulatory T cell-related lncRNAs in epilepsy and their potential role as diagnostic markers in this condition.https://www.frontiersin.org/articles/10.3389/fnmol.2022.1031314/fullepilepsylncRNATH2-LCRRMRPIFNG-AS1 (NEST)MAFTRR |
| spellingShingle | Guive Sharifi Reyhane Eghtedarian Mohammad Taheri Mohammad Taheri Bashdar Mahmud Hussen Bashdar Mahmud Hussen Solat Eslami Solat Eslami Soudeh Ghafouri-Fard Arezou Sayad Assessment of Treg-related lncRNAs in epilepsy Frontiers in Molecular Neuroscience epilepsy lncRNA TH2-LCR RMRP IFNG-AS1 (NEST) MAFTRR |
| title | Assessment of Treg-related lncRNAs in epilepsy |
| title_full | Assessment of Treg-related lncRNAs in epilepsy |
| title_fullStr | Assessment of Treg-related lncRNAs in epilepsy |
| title_full_unstemmed | Assessment of Treg-related lncRNAs in epilepsy |
| title_short | Assessment of Treg-related lncRNAs in epilepsy |
| title_sort | assessment of treg related lncrnas in epilepsy |
| topic | epilepsy lncRNA TH2-LCR RMRP IFNG-AS1 (NEST) MAFTRR |
| url | https://www.frontiersin.org/articles/10.3389/fnmol.2022.1031314/full |
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