Effect of Polysaccharides from on Intestinal Mucosal Barrier of Lipopolysaccharide Challenged Mice
To investigate the role of polysaccharide from Acanthopanax senticosus (ASPS) in preventing lipopolysaccharide (LPS)-induced intestinal injury, 18 mice (at 5 wk of age) were assigned to three groups with 6 replicates of one mouse each. Mice were administrated by oral gavage with or without ASPS (300...
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Asian-Australasian Association of Animal Production Societies
2016-01-01
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| Series: | Asian-Australasian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | http://www.ajas.info/upload/pdf/ajas-29-1-134.pdf |
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| author | Jie Han Yunhe Xu Di Yang Ning Yu Zishan Bai Lianquan Bian |
| author_facet | Jie Han Yunhe Xu Di Yang Ning Yu Zishan Bai Lianquan Bian |
| author_sort | Jie Han |
| collection | DOAJ |
| description | To investigate the role of polysaccharide from Acanthopanax senticosus (ASPS) in preventing lipopolysaccharide (LPS)-induced intestinal injury, 18 mice (at 5 wk of age) were assigned to three groups with 6 replicates of one mouse each. Mice were administrated by oral gavage with or without ASPS (300 mg/kg body weight) for 14 days and were injected with saline or LPS at 15 days. Intestinal samples were collected at 4 h post-challenge. The results showed that ASPS ameliorated LPS-induced deterioration of digestive ability of LPS-challenged mice, indicated by an increase in intestinal lactase activity (45%, p<0.05), and the intestinal morphology, as proved by improved villus height (20.84%, p<0.05) and villus height:crypt depth ratio (42%, p<0.05), and lower crypt depth in jejunum (15.55%, p<0.05), as well as enhanced intestinal tight junction proteins expression involving occludin-1 (71.43%, p<0.05). ASPS also prevented intestinal inflammation response, supported by decrease in intestinal inflammatory mediators including tumor necrosis factor α (22.28%, p<0.05) and heat shock protein (HSP70) (77.42%, p<0.05). In addition, intestinal mucus layers were also improved by ASPS, as indicated by the increase in number of goblet cells (24.89%, p<0.05) and intestinal trefoil peptide (17.75%, p<0.05). Finally, ASPS facilitated mRNA expression of epidermal growth factor (100%, p<0.05) and its receptor (200%, p<0.05) gene. These results indicate that ASPS can prevent intestinal mucosal barrier injury under inflammatory conditions, which may be associated with up-regulating gene mRNA expression of epidermal growth factor and its receptor. |
| first_indexed | 2024-04-13T17:13:49Z |
| format | Article |
| id | doaj.art-55ef7571587242eebbe5a4b29969761e |
| institution | Directory Open Access Journal |
| issn | 1011-2367 1976-5517 |
| language | English |
| last_indexed | 2024-04-13T17:13:49Z |
| publishDate | 2016-01-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Asian-Australasian Journal of Animal Sciences |
| spelling | doaj.art-55ef7571587242eebbe5a4b29969761e2022-12-22T02:38:12ZengAsian-Australasian Association of Animal Production SocietiesAsian-Australasian Journal of Animal Sciences1011-23671976-55172016-01-0129113414110.5713/ajas.15.053423343Effect of Polysaccharides from on Intestinal Mucosal Barrier of Lipopolysaccharide Challenged MiceJie HanYunhe Xu0Di YangNing Yu1Zishan BaiLianquan Bian College of Animal Husbandry and Veterinary, Liaoning Medical University, Jinzhou 121-001, .China Institute of Biotechnology Research, Liaoning Academy of Agricultural Sciences, Shenyang 1108-66, .ChinaTo investigate the role of polysaccharide from Acanthopanax senticosus (ASPS) in preventing lipopolysaccharide (LPS)-induced intestinal injury, 18 mice (at 5 wk of age) were assigned to three groups with 6 replicates of one mouse each. Mice were administrated by oral gavage with or without ASPS (300 mg/kg body weight) for 14 days and were injected with saline or LPS at 15 days. Intestinal samples were collected at 4 h post-challenge. The results showed that ASPS ameliorated LPS-induced deterioration of digestive ability of LPS-challenged mice, indicated by an increase in intestinal lactase activity (45%, p<0.05), and the intestinal morphology, as proved by improved villus height (20.84%, p<0.05) and villus height:crypt depth ratio (42%, p<0.05), and lower crypt depth in jejunum (15.55%, p<0.05), as well as enhanced intestinal tight junction proteins expression involving occludin-1 (71.43%, p<0.05). ASPS also prevented intestinal inflammation response, supported by decrease in intestinal inflammatory mediators including tumor necrosis factor α (22.28%, p<0.05) and heat shock protein (HSP70) (77.42%, p<0.05). In addition, intestinal mucus layers were also improved by ASPS, as indicated by the increase in number of goblet cells (24.89%, p<0.05) and intestinal trefoil peptide (17.75%, p<0.05). Finally, ASPS facilitated mRNA expression of epidermal growth factor (100%, p<0.05) and its receptor (200%, p<0.05) gene. These results indicate that ASPS can prevent intestinal mucosal barrier injury under inflammatory conditions, which may be associated with up-regulating gene mRNA expression of epidermal growth factor and its receptor.http://www.ajas.info/upload/pdf/ajas-29-1-134.pdfHerbal ExtractIntestinal BarrierInflammationEpidermal Growth FactorPro-inflammatory CytokinesMetabolism |
| spellingShingle | Jie Han Yunhe Xu Di Yang Ning Yu Zishan Bai Lianquan Bian Effect of Polysaccharides from on Intestinal Mucosal Barrier of Lipopolysaccharide Challenged Mice Asian-Australasian Journal of Animal Sciences Herbal Extract Intestinal Barrier Inflammation Epidermal Growth Factor Pro-inflammatory Cytokines Metabolism |
| title | Effect of Polysaccharides from on Intestinal Mucosal Barrier of Lipopolysaccharide Challenged Mice |
| title_full | Effect of Polysaccharides from on Intestinal Mucosal Barrier of Lipopolysaccharide Challenged Mice |
| title_fullStr | Effect of Polysaccharides from on Intestinal Mucosal Barrier of Lipopolysaccharide Challenged Mice |
| title_full_unstemmed | Effect of Polysaccharides from on Intestinal Mucosal Barrier of Lipopolysaccharide Challenged Mice |
| title_short | Effect of Polysaccharides from on Intestinal Mucosal Barrier of Lipopolysaccharide Challenged Mice |
| title_sort | effect of polysaccharides from on intestinal mucosal barrier of lipopolysaccharide challenged mice |
| topic | Herbal Extract Intestinal Barrier Inflammation Epidermal Growth Factor Pro-inflammatory Cytokines Metabolism |
| url | http://www.ajas.info/upload/pdf/ajas-29-1-134.pdf |
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