Unlocking Potentially Therapeutic Phytochemicals in Capadulla (<i>Doliocarpus dentatus</i>) from Guyana Using Untargeted Mass Spectrometry-Based Metabolomics
<i>Doliocarpus dentatus</i> is thought to have a wide variety of therapeutic phytochemicals that allegedly improve libido and cure impotence. Although a few biomarkers have been identified with potential antinociceptive and cytotoxic properties, an untargeted mass spectrometry-based meta...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-10-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/13/10/1050 |
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| author | Ewart Smith Ainsely Lewis Suresh S. Narine R. J. Neil Emery |
| author_facet | Ewart Smith Ainsely Lewis Suresh S. Narine R. J. Neil Emery |
| author_sort | Ewart Smith |
| collection | DOAJ |
| description | <i>Doliocarpus dentatus</i> is thought to have a wide variety of therapeutic phytochemicals that allegedly improve libido and cure impotence. Although a few biomarkers have been identified with potential antinociceptive and cytotoxic properties, an untargeted mass spectrometry-based metabolomics approach has never been undertaken to identify therapeutic biofingerprints for conditions, such as erectile dysfunction, in men. This study executes a preliminary phytochemical screening of the woody vine of two ecotypes of <i>D. dentatus</i> with renowned differences in therapeutic potential for erectile dysfunction. Liquid chromatography–mass spectrometry-based metabolomics was used to screen for flavonoids, terpenoids, and other chemical classes found to contrast between red and white ecotypes. Among the metabolite chemodiversity found in the ecotype screens, using a combination of GNPS, MS-DIAL, and SIRIUS, approximately 847 compounds were annotated at levels 2 to 4, with the majority of compounds falling under lipid and lipid-like molecules, benzenoids and phenylpropanoids, and polyketides, indicative of the contributions of the flavonoid, shikimic acid, and terpenoid biosynthesis pathways. Despite the extensive annotation, we report on 138 tentative compound identifications of potentially therapeutic compounds, with 55 selected compounds at a level-2 annotation, and 22 statistically significant therapeutic biomarkers, the majority of which were polyphenols. Epicatechin methyl gallate, catechin gallate, and proanthocyanidin A2 had the greatest significant differences and were also relatively abundant among the red and white ecotypes. These putatively identified compounds reportedly act as antioxidants, neutralizing damaging free radicals, and lowering cell oxidative stress, thus aiding in potentially preventing cellular damage and promoting overall well-being, especially for treating erectile dysfunction (ED). |
| first_indexed | 2024-03-10T21:03:26Z |
| format | Article |
| id | doaj.art-55f085c3bac145629fc3eb81ebf7e19b |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-10T21:03:26Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-55f085c3bac145629fc3eb81ebf7e19b2023-11-19T17:19:45ZengMDPI AGMetabolites2218-19892023-10-011310105010.3390/metabo13101050Unlocking Potentially Therapeutic Phytochemicals in Capadulla (<i>Doliocarpus dentatus</i>) from Guyana Using Untargeted Mass Spectrometry-Based MetabolomicsEwart Smith0Ainsely Lewis1Suresh S. Narine2R. J. Neil Emery3Environmental and Life Sciences Graduate Program, Trent University, Peterborough, ON K9J 0G2, CanadaDepartment of Biology, Trent University, Peterborough, ON K9J 0G2, CanadaTrent Centre for Biomaterials Research, Trent University, Peterborough, ON K9J 0G2, CanadaDepartment of Biology, Trent University, Peterborough, ON K9J 0G2, Canada<i>Doliocarpus dentatus</i> is thought to have a wide variety of therapeutic phytochemicals that allegedly improve libido and cure impotence. Although a few biomarkers have been identified with potential antinociceptive and cytotoxic properties, an untargeted mass spectrometry-based metabolomics approach has never been undertaken to identify therapeutic biofingerprints for conditions, such as erectile dysfunction, in men. This study executes a preliminary phytochemical screening of the woody vine of two ecotypes of <i>D. dentatus</i> with renowned differences in therapeutic potential for erectile dysfunction. Liquid chromatography–mass spectrometry-based metabolomics was used to screen for flavonoids, terpenoids, and other chemical classes found to contrast between red and white ecotypes. Among the metabolite chemodiversity found in the ecotype screens, using a combination of GNPS, MS-DIAL, and SIRIUS, approximately 847 compounds were annotated at levels 2 to 4, with the majority of compounds falling under lipid and lipid-like molecules, benzenoids and phenylpropanoids, and polyketides, indicative of the contributions of the flavonoid, shikimic acid, and terpenoid biosynthesis pathways. Despite the extensive annotation, we report on 138 tentative compound identifications of potentially therapeutic compounds, with 55 selected compounds at a level-2 annotation, and 22 statistically significant therapeutic biomarkers, the majority of which were polyphenols. Epicatechin methyl gallate, catechin gallate, and proanthocyanidin A2 had the greatest significant differences and were also relatively abundant among the red and white ecotypes. These putatively identified compounds reportedly act as antioxidants, neutralizing damaging free radicals, and lowering cell oxidative stress, thus aiding in potentially preventing cellular damage and promoting overall well-being, especially for treating erectile dysfunction (ED).https://www.mdpi.com/2218-1989/13/10/1050Capadullaerectile dysfunctionmass spectrometrymetabolomicspolyphenolsantioxidants |
| spellingShingle | Ewart Smith Ainsely Lewis Suresh S. Narine R. J. Neil Emery Unlocking Potentially Therapeutic Phytochemicals in Capadulla (<i>Doliocarpus dentatus</i>) from Guyana Using Untargeted Mass Spectrometry-Based Metabolomics Metabolites Capadulla erectile dysfunction mass spectrometry metabolomics polyphenols antioxidants |
| title | Unlocking Potentially Therapeutic Phytochemicals in Capadulla (<i>Doliocarpus dentatus</i>) from Guyana Using Untargeted Mass Spectrometry-Based Metabolomics |
| title_full | Unlocking Potentially Therapeutic Phytochemicals in Capadulla (<i>Doliocarpus dentatus</i>) from Guyana Using Untargeted Mass Spectrometry-Based Metabolomics |
| title_fullStr | Unlocking Potentially Therapeutic Phytochemicals in Capadulla (<i>Doliocarpus dentatus</i>) from Guyana Using Untargeted Mass Spectrometry-Based Metabolomics |
| title_full_unstemmed | Unlocking Potentially Therapeutic Phytochemicals in Capadulla (<i>Doliocarpus dentatus</i>) from Guyana Using Untargeted Mass Spectrometry-Based Metabolomics |
| title_short | Unlocking Potentially Therapeutic Phytochemicals in Capadulla (<i>Doliocarpus dentatus</i>) from Guyana Using Untargeted Mass Spectrometry-Based Metabolomics |
| title_sort | unlocking potentially therapeutic phytochemicals in capadulla i doliocarpus dentatus i from guyana using untargeted mass spectrometry based metabolomics |
| topic | Capadulla erectile dysfunction mass spectrometry metabolomics polyphenols antioxidants |
| url | https://www.mdpi.com/2218-1989/13/10/1050 |
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