Chlamydia trachomatis isolated from cervicovaginal samples in Sapporo, Japan, reveals the circulation of genetically diverse strains
Abstract Background This study was conducted to understand the molecular epidemiology of circulating Chlamydia trachomatis (Ct) strains in Sapporo, Japan. Methods A total of 713 endocervical samples collected from April 2016 to March 2019 were screened for Ct. The obtained Ct positive samples were a...
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| Format: | Article |
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BMC
2020-01-01
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| Series: | BMC Infectious Diseases |
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| Online Access: | https://doi.org/10.1186/s12879-020-4780-y |
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| author | Jeewan Thapa Takanori Watanabe Mana Isoba Torahiko Okubo Kiyotake Abe Kunihiro Minami Hiroyuki Yamaguchi |
| author_facet | Jeewan Thapa Takanori Watanabe Mana Isoba Torahiko Okubo Kiyotake Abe Kunihiro Minami Hiroyuki Yamaguchi |
| author_sort | Jeewan Thapa |
| collection | DOAJ |
| description | Abstract Background This study was conducted to understand the molecular epidemiology of circulating Chlamydia trachomatis (Ct) strains in Sapporo, Japan. Methods A total of 713 endocervical samples collected from April 2016 to March 2019 were screened for Ct. The obtained Ct positive samples were analyzed by ompA genotyping and multilocus sequence analysis (MLSA). Results Eighty-three (11.6%) samples were positive for Ct plasmid DNA. Sequence analysis of the ompA gene from the 61 positive cases revealed eight genotypes: F (40.9%), E (19.6%), D (14.7%), G (9.8%), H (6.5%), I (3.2%), K (3.2%), and J (1.6%). The globally dominant genotype E and F strains were highly conserved with 13 ompA genetic variants being detected, whereas genotype D strains were the most diverse. Genetic characterization of D strains revealed that D1 genetic variants may be potentially specific to Sapporo. MLSA revealed 13 unique sequence types (STs) including four novel STs from 53 positive samples, with the globally dominant STs 39 and 19 being predominant. STs 39, 34, and 21 were exclusively associated with genotypes E and F indicating their global dominance. Novel ST70 and ST30 were specifically associated with genotype D. Conclusion Our study has revealed the circulation of genetically diverse Ct strains in the women population of Sapporo, Japan. We suggest identifying a transmission network of those successful strains and implementing public health prevention strategies to control the spread of Ct in Sapporo. |
| first_indexed | 2024-12-14T06:15:15Z |
| format | Article |
| id | doaj.art-55f1df39db5f463ab62cf01578ccaa60 |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-12-14T06:15:15Z |
| publishDate | 2020-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj.art-55f1df39db5f463ab62cf01578ccaa602022-12-21T23:14:02ZengBMCBMC Infectious Diseases1471-23342020-01-012011910.1186/s12879-020-4780-yChlamydia trachomatis isolated from cervicovaginal samples in Sapporo, Japan, reveals the circulation of genetically diverse strainsJeewan Thapa0Takanori Watanabe1Mana Isoba2Torahiko Okubo3Kiyotake Abe4Kunihiro Minami5Hiroyuki Yamaguchi6Department of Medical Laboratory Science, Faculty of Health Sciences, Hokkaido UniversityDepartment of Medical Laboratory Science, Faculty of Health Sciences, Hokkaido UniversityDepartment of Medical Laboratory Science, Faculty of Health Sciences, Hokkaido UniversityDepartment of Medical Laboratory Science, Faculty of Health Sciences, Hokkaido UniversityToho Obstetrics and Gynecology HospitalToho Obstetrics and Gynecology HospitalDepartment of Medical Laboratory Science, Faculty of Health Sciences, Hokkaido UniversityAbstract Background This study was conducted to understand the molecular epidemiology of circulating Chlamydia trachomatis (Ct) strains in Sapporo, Japan. Methods A total of 713 endocervical samples collected from April 2016 to March 2019 were screened for Ct. The obtained Ct positive samples were analyzed by ompA genotyping and multilocus sequence analysis (MLSA). Results Eighty-three (11.6%) samples were positive for Ct plasmid DNA. Sequence analysis of the ompA gene from the 61 positive cases revealed eight genotypes: F (40.9%), E (19.6%), D (14.7%), G (9.8%), H (6.5%), I (3.2%), K (3.2%), and J (1.6%). The globally dominant genotype E and F strains were highly conserved with 13 ompA genetic variants being detected, whereas genotype D strains were the most diverse. Genetic characterization of D strains revealed that D1 genetic variants may be potentially specific to Sapporo. MLSA revealed 13 unique sequence types (STs) including four novel STs from 53 positive samples, with the globally dominant STs 39 and 19 being predominant. STs 39, 34, and 21 were exclusively associated with genotypes E and F indicating their global dominance. Novel ST70 and ST30 were specifically associated with genotype D. Conclusion Our study has revealed the circulation of genetically diverse Ct strains in the women population of Sapporo, Japan. We suggest identifying a transmission network of those successful strains and implementing public health prevention strategies to control the spread of Ct in Sapporo.https://doi.org/10.1186/s12879-020-4780-yChlamydia trachomatisompAMultilocus sequence analysisGenotypes |
| spellingShingle | Jeewan Thapa Takanori Watanabe Mana Isoba Torahiko Okubo Kiyotake Abe Kunihiro Minami Hiroyuki Yamaguchi Chlamydia trachomatis isolated from cervicovaginal samples in Sapporo, Japan, reveals the circulation of genetically diverse strains BMC Infectious Diseases Chlamydia trachomatis ompA Multilocus sequence analysis Genotypes |
| title | Chlamydia trachomatis isolated from cervicovaginal samples in Sapporo, Japan, reveals the circulation of genetically diverse strains |
| title_full | Chlamydia trachomatis isolated from cervicovaginal samples in Sapporo, Japan, reveals the circulation of genetically diverse strains |
| title_fullStr | Chlamydia trachomatis isolated from cervicovaginal samples in Sapporo, Japan, reveals the circulation of genetically diverse strains |
| title_full_unstemmed | Chlamydia trachomatis isolated from cervicovaginal samples in Sapporo, Japan, reveals the circulation of genetically diverse strains |
| title_short | Chlamydia trachomatis isolated from cervicovaginal samples in Sapporo, Japan, reveals the circulation of genetically diverse strains |
| title_sort | chlamydia trachomatis isolated from cervicovaginal samples in sapporo japan reveals the circulation of genetically diverse strains |
| topic | Chlamydia trachomatis ompA Multilocus sequence analysis Genotypes |
| url | https://doi.org/10.1186/s12879-020-4780-y |
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