Antimicrobial Susceptibility among Urban Wastewater and Wild Shellfish Isolates of Non-O1/Non-O139 Vibrio cholerae from La Rance Estuary (Brittany, France)
The early 2000s marked the end of the Golden age of the antibiotics and the beginning of the awareness on the potential threat to human health due to the dissemination of antimicrobial resistance. As a base-line study, we investigated the antimicrobial susceptibility of 99 strains of non-O1/non-O139...
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Frontiers Media S.A.
2017-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2017.01637/full |
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| author | Sandrine Baron Sandrine Baron Emeline Larvor Emeline Larvor Séverine Chevalier Séverine Chevalier Eric Jouy Eric Jouy Isabelle Kempf Isabelle Kempf Sophie A. Granier Jean Lesne Jean Lesne |
| author_facet | Sandrine Baron Sandrine Baron Emeline Larvor Emeline Larvor Séverine Chevalier Séverine Chevalier Eric Jouy Eric Jouy Isabelle Kempf Isabelle Kempf Sophie A. Granier Jean Lesne Jean Lesne |
| author_sort | Sandrine Baron |
| collection | DOAJ |
| description | The early 2000s marked the end of the Golden age of the antibiotics and the beginning of the awareness on the potential threat to human health due to the dissemination of antimicrobial resistance. As a base-line study, we investigated the antimicrobial susceptibility of 99 strains of non-O1/non-O139 Vibrio cholerae isolated from wastewater and shellfish in 2000/2001 within La Rance estuary (Brittany, France). All isolates were susceptible to amoxicillin-clavulanic acid, cefotaxime, imipenem, chloramphenicol, nalidixic acid, ciprofloxacin, norfloxacin, amikacin, gentamicin, tetracycline, doxycycline, trimethoprim-sulfamethoxazole, and erythromycin. The only resistances were to streptomycin, sulfonamides and ampicillin: 54.6% of the isolates had acquired resistance to at least one antimicrobial agent among them and only six isolates from cockles were multidrug resistant. On the basis of the distribution of a limited selection of resistance associated genes, our study shows that V. cholerae can constitute an environmental reservoir for these genes. However, none of our isolates harbored integron. This result casts doubt on the capacity of non-O1/non-O139 V. cholerae to acquire resistance-associated genes in such context, and on its potential role of indicator of the dissemination of antimicrobial resistance in the aquatic environment. |
| first_indexed | 2024-12-14T14:23:10Z |
| format | Article |
| id | doaj.art-55f506905f5f4666bd52b391e1b892dd |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-14T14:23:10Z |
| publishDate | 2017-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-55f506905f5f4666bd52b391e1b892dd2022-12-21T22:58:01ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-09-01810.3389/fmicb.2017.01637284629Antimicrobial Susceptibility among Urban Wastewater and Wild Shellfish Isolates of Non-O1/Non-O139 Vibrio cholerae from La Rance Estuary (Brittany, France)Sandrine Baron0Sandrine Baron1Emeline Larvor2Emeline Larvor3Séverine Chevalier4Séverine Chevalier5Eric Jouy6Eric Jouy7Isabelle Kempf8Isabelle Kempf9Sophie A. Granier10Jean Lesne11Jean Lesne12Mycoplasmology-Bacteriology Unit, Ploufragan-Plouzané Laboratory, French Agency for Food, Environmental and Occupational Health and Safety (Anses)Ploufragan, FranceBretagne-Loire UniversityRennes, FranceMycoplasmology-Bacteriology Unit, Ploufragan-Plouzané Laboratory, French Agency for Food, Environmental and Occupational Health and Safety (Anses)Ploufragan, FranceBretagne-Loire UniversityRennes, FranceBretagne-Loire UniversityRennes, FranceEcole des Hautes Etudes en Santé Publique, Laboratoire d'Etude et de Recherche en Environnement et Santé, Institut de Recherche en Santé, Environnement et Travail, UMR 1085Rennes, FranceMycoplasmology-Bacteriology Unit, Ploufragan-Plouzané Laboratory, French Agency for Food, Environmental and Occupational Health and Safety (Anses)Ploufragan, FranceBretagne-Loire UniversityRennes, FranceMycoplasmology-Bacteriology Unit, Ploufragan-Plouzané Laboratory, French Agency for Food, Environmental and Occupational Health and Safety (Anses)Ploufragan, FranceBretagne-Loire UniversityRennes, FranceLaboratory for Food Safety, French Agency for Food, Environmental and Occupational Health and Safety (Anses), Paris-Est UniversityMaisons-Alfort, FranceBretagne-Loire UniversityRennes, FranceEcole des Hautes Etudes en Santé Publique, Laboratoire d'Etude et de Recherche en Environnement et Santé, Institut de Recherche en Santé, Environnement et Travail, UMR 1085Rennes, FranceThe early 2000s marked the end of the Golden age of the antibiotics and the beginning of the awareness on the potential threat to human health due to the dissemination of antimicrobial resistance. As a base-line study, we investigated the antimicrobial susceptibility of 99 strains of non-O1/non-O139 Vibrio cholerae isolated from wastewater and shellfish in 2000/2001 within La Rance estuary (Brittany, France). All isolates were susceptible to amoxicillin-clavulanic acid, cefotaxime, imipenem, chloramphenicol, nalidixic acid, ciprofloxacin, norfloxacin, amikacin, gentamicin, tetracycline, doxycycline, trimethoprim-sulfamethoxazole, and erythromycin. The only resistances were to streptomycin, sulfonamides and ampicillin: 54.6% of the isolates had acquired resistance to at least one antimicrobial agent among them and only six isolates from cockles were multidrug resistant. On the basis of the distribution of a limited selection of resistance associated genes, our study shows that V. cholerae can constitute an environmental reservoir for these genes. However, none of our isolates harbored integron. This result casts doubt on the capacity of non-O1/non-O139 V. cholerae to acquire resistance-associated genes in such context, and on its potential role of indicator of the dissemination of antimicrobial resistance in the aquatic environment.http://journal.frontiersin.org/article/10.3389/fmicb.2017.01637/fullVibrio cholerae non-O1/non-O139wastewaterwild shellfishantimicrobial resistanceestuary |
| spellingShingle | Sandrine Baron Sandrine Baron Emeline Larvor Emeline Larvor Séverine Chevalier Séverine Chevalier Eric Jouy Eric Jouy Isabelle Kempf Isabelle Kempf Sophie A. Granier Jean Lesne Jean Lesne Antimicrobial Susceptibility among Urban Wastewater and Wild Shellfish Isolates of Non-O1/Non-O139 Vibrio cholerae from La Rance Estuary (Brittany, France) Frontiers in Microbiology Vibrio cholerae non-O1/non-O139 wastewater wild shellfish antimicrobial resistance estuary |
| title | Antimicrobial Susceptibility among Urban Wastewater and Wild Shellfish Isolates of Non-O1/Non-O139 Vibrio cholerae from La Rance Estuary (Brittany, France) |
| title_full | Antimicrobial Susceptibility among Urban Wastewater and Wild Shellfish Isolates of Non-O1/Non-O139 Vibrio cholerae from La Rance Estuary (Brittany, France) |
| title_fullStr | Antimicrobial Susceptibility among Urban Wastewater and Wild Shellfish Isolates of Non-O1/Non-O139 Vibrio cholerae from La Rance Estuary (Brittany, France) |
| title_full_unstemmed | Antimicrobial Susceptibility among Urban Wastewater and Wild Shellfish Isolates of Non-O1/Non-O139 Vibrio cholerae from La Rance Estuary (Brittany, France) |
| title_short | Antimicrobial Susceptibility among Urban Wastewater and Wild Shellfish Isolates of Non-O1/Non-O139 Vibrio cholerae from La Rance Estuary (Brittany, France) |
| title_sort | antimicrobial susceptibility among urban wastewater and wild shellfish isolates of non o1 non o139 vibrio cholerae from la rance estuary brittany france |
| topic | Vibrio cholerae non-O1/non-O139 wastewater wild shellfish antimicrobial resistance estuary |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2017.01637/full |
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