Transport from the wild rapidly alters the diversity and composition of skin microbial communities and antifungal taxa in spring peeper frogs
Amphibians are routinely collected from the wild and added into managed care and public display facilities; however, there is a gap in understanding how these practices might alter the diversity and composition of skin microbial communities on these animals. The aim of this study was to evaluate and...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-04-01
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| Series: | Frontiers in Microbiomes |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/frmbi.2024.1368538/full |
| _version_ | 1797201247623708672 |
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| author | Lauren P. Kane Lauren P. Kane William G. Van Bonn Francis J. Oliaro Christian F. Edwardson Malissa Smith Lee J. Pinnell |
| author_facet | Lauren P. Kane Lauren P. Kane William G. Van Bonn Francis J. Oliaro Christian F. Edwardson Malissa Smith Lee J. Pinnell |
| author_sort | Lauren P. Kane |
| collection | DOAJ |
| description | Amphibians are routinely collected from the wild and added into managed care and public display facilities; however, there is a gap in understanding how these practices might alter the diversity and composition of skin microbial communities on these animals. The aim of this study was to evaluate and compare skin microbial communities of spring peeper frogs (Pseudacris crucifer) from acquisition in the wild through the end of their quarantine period and identify microbial taxa with antifungal properties. From an original group of seventy-six frogs, cohorts of ten were swabbed when acquired in the wild, upon transport from the wild, and swabbed throughout a 9-week quarantine period while under managed care. An immediate loss of microbial richness and diversity was evident upon transfer of the frogs from their original environment and continued throughout subsequent sampling time-points during quarantine. Importantly, antifungal taxa comprised significantly more of the overall skin community after the frogs were moved from the wild, largely due to members of the family Moraxellaceae. Overall, our findings demonstrate that amphibian skin microbiome changes immediately on removal from the wild, and that these changes persist throughout quarantine while being housed under managed care. This may play a pivotal role in the development of dermatological disease and have implications in the health and immune function of amphibians. |
| first_indexed | 2024-04-24T07:44:31Z |
| format | Article |
| id | doaj.art-55fb4735807b416e8815e85818c0c6cd |
| institution | Directory Open Access Journal |
| issn | 2813-4338 |
| language | English |
| last_indexed | 2024-04-24T07:44:31Z |
| publishDate | 2024-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiomes |
| spelling | doaj.art-55fb4735807b416e8815e85818c0c6cd2024-04-19T04:50:40ZengFrontiers Media S.A.Frontiers in Microbiomes2813-43382024-04-01310.3389/frmbi.2024.13685381368538Transport from the wild rapidly alters the diversity and composition of skin microbial communities and antifungal taxa in spring peeper frogsLauren P. Kane0Lauren P. Kane1William G. Van Bonn2Francis J. Oliaro3Christian F. Edwardson4Malissa Smith5Lee J. Pinnell6Animal Care and Science Division, John G. Shedd Aquarium, Chicago, IL, United StatesDepartment of Animal Health, Pittsburgh Zoo & Aquarium, Pittsburgh, PA, United StatesAnimal Care and Science Division, John G. Shedd Aquarium, Chicago, IL, United StatesAnimal Care and Science Division, John G. Shedd Aquarium, Chicago, IL, United StatesAnimal Care and Science Division, John G. Shedd Aquarium, Chicago, IL, United StatesDepartment of Aquatic Sustainability, Georgia Aquarium, Atlanta, GA, United StatesVeterinary Education, Research, and Outreach Program, Texas A&M University, Canyon, TX, United StatesAmphibians are routinely collected from the wild and added into managed care and public display facilities; however, there is a gap in understanding how these practices might alter the diversity and composition of skin microbial communities on these animals. The aim of this study was to evaluate and compare skin microbial communities of spring peeper frogs (Pseudacris crucifer) from acquisition in the wild through the end of their quarantine period and identify microbial taxa with antifungal properties. From an original group of seventy-six frogs, cohorts of ten were swabbed when acquired in the wild, upon transport from the wild, and swabbed throughout a 9-week quarantine period while under managed care. An immediate loss of microbial richness and diversity was evident upon transfer of the frogs from their original environment and continued throughout subsequent sampling time-points during quarantine. Importantly, antifungal taxa comprised significantly more of the overall skin community after the frogs were moved from the wild, largely due to members of the family Moraxellaceae. Overall, our findings demonstrate that amphibian skin microbiome changes immediately on removal from the wild, and that these changes persist throughout quarantine while being housed under managed care. This may play a pivotal role in the development of dermatological disease and have implications in the health and immune function of amphibians.https://www.frontiersin.org/articles/10.3389/frmbi.2024.1368538/fullamphibianmicrobiomeskinantifungal taxamanaged environment |
| spellingShingle | Lauren P. Kane Lauren P. Kane William G. Van Bonn Francis J. Oliaro Christian F. Edwardson Malissa Smith Lee J. Pinnell Transport from the wild rapidly alters the diversity and composition of skin microbial communities and antifungal taxa in spring peeper frogs Frontiers in Microbiomes amphibian microbiome skin antifungal taxa managed environment |
| title | Transport from the wild rapidly alters the diversity and composition of skin microbial communities and antifungal taxa in spring peeper frogs |
| title_full | Transport from the wild rapidly alters the diversity and composition of skin microbial communities and antifungal taxa in spring peeper frogs |
| title_fullStr | Transport from the wild rapidly alters the diversity and composition of skin microbial communities and antifungal taxa in spring peeper frogs |
| title_full_unstemmed | Transport from the wild rapidly alters the diversity and composition of skin microbial communities and antifungal taxa in spring peeper frogs |
| title_short | Transport from the wild rapidly alters the diversity and composition of skin microbial communities and antifungal taxa in spring peeper frogs |
| title_sort | transport from the wild rapidly alters the diversity and composition of skin microbial communities and antifungal taxa in spring peeper frogs |
| topic | amphibian microbiome skin antifungal taxa managed environment |
| url | https://www.frontiersin.org/articles/10.3389/frmbi.2024.1368538/full |
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