Viral infection impacts the 3D subcellular structure of the abundant marine diatom Guinardia delicatula
Viruses are key players in marine ecosystems where they infect abundant marine microbes. RNA viruses are emerging as key members of the marine virosphere. They have recently been identified as a potential source of mortality in diatoms, a group of microalgae that accounts for roughly 40% of the prim...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Marine Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2022.1034235/full |
| _version_ | 1811169354920755200 |
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| author | Marie Walde Cyprien Camplong Colomban de Vargas Colomban de Vargas Anne-Claire Baudoux Nathalie Simon |
| author_facet | Marie Walde Cyprien Camplong Colomban de Vargas Colomban de Vargas Anne-Claire Baudoux Nathalie Simon |
| author_sort | Marie Walde |
| collection | DOAJ |
| description | Viruses are key players in marine ecosystems where they infect abundant marine microbes. RNA viruses are emerging as key members of the marine virosphere. They have recently been identified as a potential source of mortality in diatoms, a group of microalgae that accounts for roughly 40% of the primary production in the ocean. Despite their likely importance, their impacts on host populations and ecosystems remain difficult to assess. In this study, we introduce an innovative approach that combines automated 3D confocal microscopy with quantitative image analysis and physiological measurements to expand our understanding of viral infection. We followed different stages of infection of the bloom-forming diatom Guinardia delicatula by the RNA virus GdelRNAV-04 until the complete lysis of the host. From 20h after infection, we observed quantifiable changes in subcellular host morphology and biomass. Our microscopy monitoring also showed that viral infection of G. delicatula induced the formation of auxospores as a probable defense strategy against viruses. Our method enables the detection of discriminative morphological features on the subcellular scale and at high throughput for comparing populations, making it a promising approach for the quantification of viral infections in the field in the future. |
| first_indexed | 2024-04-10T16:41:00Z |
| format | Article |
| id | doaj.art-55fb9cee772648429c7f38c00ef172e6 |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-04-10T16:41:00Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-55fb9cee772648429c7f38c00ef172e62023-02-08T06:44:37ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452023-02-01910.3389/fmars.2022.10342351034235Viral infection impacts the 3D subcellular structure of the abundant marine diatom Guinardia delicatulaMarie Walde0Cyprien Camplong1Colomban de Vargas2Colomban de Vargas3Anne-Claire Baudoux4Nathalie Simon5Sorbonne Université, Centre National de la Recherche Scientifique (CNRS), Laboratoire Adaptation et Diversité en Milieu Marin UMR7144, Station Biologique de Roscoff, Roscoff, FranceSorbonne Université, Centre National de la Recherche Scientifique (CNRS), Laboratoire Adaptation et Diversité en Milieu Marin UMR7144, Station Biologique de Roscoff, Roscoff, FranceSorbonne Université, Centre National de la Recherche Scientifique (CNRS), Laboratoire Adaptation et Diversité en Milieu Marin UMR7144, Station Biologique de Roscoff, Roscoff, FranceResearch Federation for the Study of Global Ocean Systems Ecology and Evolution, FR2022/Tara GOSEE, Paris, FranceSorbonne Université, Centre National de la Recherche Scientifique (CNRS), Laboratoire Adaptation et Diversité en Milieu Marin UMR7144, Station Biologique de Roscoff, Roscoff, FranceSorbonne Université, Centre National de la Recherche Scientifique (CNRS), Laboratoire Adaptation et Diversité en Milieu Marin UMR7144, Station Biologique de Roscoff, Roscoff, FranceViruses are key players in marine ecosystems where they infect abundant marine microbes. RNA viruses are emerging as key members of the marine virosphere. They have recently been identified as a potential source of mortality in diatoms, a group of microalgae that accounts for roughly 40% of the primary production in the ocean. Despite their likely importance, their impacts on host populations and ecosystems remain difficult to assess. In this study, we introduce an innovative approach that combines automated 3D confocal microscopy with quantitative image analysis and physiological measurements to expand our understanding of viral infection. We followed different stages of infection of the bloom-forming diatom Guinardia delicatula by the RNA virus GdelRNAV-04 until the complete lysis of the host. From 20h after infection, we observed quantifiable changes in subcellular host morphology and biomass. Our microscopy monitoring also showed that viral infection of G. delicatula induced the formation of auxospores as a probable defense strategy against viruses. Our method enables the detection of discriminative morphological features on the subcellular scale and at high throughput for comparing populations, making it a promising approach for the quantification of viral infections in the field in the future.https://www.frontiersin.org/articles/10.3389/fmars.2022.1034235/fullGuinardia delicatulaviral infectionmorphometryautomated microscopy applicationsmarine diatomsmarine plankton |
| spellingShingle | Marie Walde Cyprien Camplong Colomban de Vargas Colomban de Vargas Anne-Claire Baudoux Nathalie Simon Viral infection impacts the 3D subcellular structure of the abundant marine diatom Guinardia delicatula Frontiers in Marine Science Guinardia delicatula viral infection morphometry automated microscopy applications marine diatoms marine plankton |
| title | Viral infection impacts the 3D subcellular structure of the abundant marine diatom Guinardia delicatula |
| title_full | Viral infection impacts the 3D subcellular structure of the abundant marine diatom Guinardia delicatula |
| title_fullStr | Viral infection impacts the 3D subcellular structure of the abundant marine diatom Guinardia delicatula |
| title_full_unstemmed | Viral infection impacts the 3D subcellular structure of the abundant marine diatom Guinardia delicatula |
| title_short | Viral infection impacts the 3D subcellular structure of the abundant marine diatom Guinardia delicatula |
| title_sort | viral infection impacts the 3d subcellular structure of the abundant marine diatom guinardia delicatula |
| topic | Guinardia delicatula viral infection morphometry automated microscopy applications marine diatoms marine plankton |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2022.1034235/full |
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