Essential Oil Blends with or without Fumaric Acid Influenced In Vitro Rumen Fermentation, Greenhouse Gas Emission, and Volatile Fatty Acids Production of a Total Mixed Ration
The growing interest in improving rumen fermentation and mitigating methane emissions necessitates the use of essential oil blends (EOB) and fumaric acid (FA). This study evaluated the synergistic effect of four EOB with or without FA supplementation on in vitro dry matter digestibility, greenhouse...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | Ruminants |
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| Online Access: | https://www.mdpi.com/2673-933X/3/4/31 |
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| author | Joel O. Alabi Deborah O. Okedoyin Chika C. Anotaenwere Michael Wuaku DeAndrea Gray Oludotun O. Adelusi Kelechi A. Ike Lydia K. Olagunju Peter A. Dele Uchenna Y. Anele |
| author_facet | Joel O. Alabi Deborah O. Okedoyin Chika C. Anotaenwere Michael Wuaku DeAndrea Gray Oludotun O. Adelusi Kelechi A. Ike Lydia K. Olagunju Peter A. Dele Uchenna Y. Anele |
| author_sort | Joel O. Alabi |
| collection | DOAJ |
| description | The growing interest in improving rumen fermentation and mitigating methane emissions necessitates the use of essential oil blends (EOB) and fumaric acid (FA). This study evaluated the synergistic effect of four EOB with or without FA supplementation on in vitro dry matter digestibility, greenhouse gas emission, and total volatile fatty acid production using inoculum from three rumen-cannulated Black Angus beef cows. The study was arranged in a 4 × 2 + 1 factorial design to evaluate the effects of the four EOB and two FA levels on a total mixed ration (TMR). The EOB dosage was 100 µL while FA was added at 3% of total mixed ration. The EOB × FA interaction (<i>p</i> < 0.05) influenced the dry matter, neutral detergent fiber, and hemicellulose degradabilities. All the EOB and FA (EFA) treatments decreased (<i>p</i> < 0.001) the dry matter degradability compared to the control (TMR substrate only). The EFA4 treatment reduced the neutral detergent fiber and hemicellulose degradabilities compared to the control. The ruminal pH was influenced (<i>p</i> < 0.001) by both the EOB and FA inclusion, and the EOB × FA interaction was significant. The microbial mass was higher (<i>p</i> < 0.001) in the EFA1, EFA4, and EOB4 compared to the control and the EOB3 treatments. The EFA1 and EOB1 produced less (<i>p</i> < 0.001) gas than the control by 29.1 and 32.1%, respectively. Compared with the control, the EFA1 and EOB1 treatments decreased (<i>p</i> < 0.001) methane gas by 90.8% and 86.4%, respectively, while the carbon dioxide was reduced (<i>p</i> = 0.004) by 65.7 and 57.9%, respectively. The EOB × FA interaction was significant (<i>p</i> < 0.001) for the total and individual volatile fatty acid concentrations. The inclusion of FA increased the propionate concentration by 9.5% and decreased (<i>p</i> = 0.02) the acetate concentration by 4%. In summary, the synergistic effect of the EOB and FA offers an effective way to reduce greenhouse gas emission and enhance total volatile fatty acids. |
| first_indexed | 2024-03-08T20:22:58Z |
| format | Article |
| id | doaj.art-55fc5f563dd5438a98f4df826a5920e6 |
| institution | Directory Open Access Journal |
| issn | 2673-933X |
| language | English |
| last_indexed | 2024-03-08T20:22:58Z |
| publishDate | 2023-11-01 |
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| series | Ruminants |
| spelling | doaj.art-55fc5f563dd5438a98f4df826a5920e62023-12-22T14:39:41ZengMDPI AGRuminants2673-933X2023-11-013437338410.3390/ruminants3040031Essential Oil Blends with or without Fumaric Acid Influenced In Vitro Rumen Fermentation, Greenhouse Gas Emission, and Volatile Fatty Acids Production of a Total Mixed RationJoel O. Alabi0Deborah O. Okedoyin1Chika C. Anotaenwere2Michael Wuaku3DeAndrea Gray4Oludotun O. Adelusi5Kelechi A. Ike6Lydia K. Olagunju7Peter A. Dele8Uchenna Y. Anele9Department of Animal Sciences, North Carolina Agricultural and Technical State University, Greensboro, NC 24711, USADepartment of Animal Sciences, North Carolina Agricultural and Technical State University, Greensboro, NC 24711, USADepartment of Animal Sciences, North Carolina Agricultural and Technical State University, Greensboro, NC 24711, USADepartment of Animal Sciences, North Carolina Agricultural and Technical State University, Greensboro, NC 24711, USADepartment of Animal Sciences, North Carolina Agricultural and Technical State University, Greensboro, NC 24711, USADepartment of Animal Sciences, North Carolina Agricultural and Technical State University, Greensboro, NC 24711, USADepartment of Animal Sciences, North Carolina Agricultural and Technical State University, Greensboro, NC 24711, USADepartment of Animal Sciences, North Carolina Agricultural and Technical State University, Greensboro, NC 24711, USADepartment of Animal Sciences, North Carolina Agricultural and Technical State University, Greensboro, NC 24711, USADepartment of Animal Sciences, North Carolina Agricultural and Technical State University, Greensboro, NC 24711, USAThe growing interest in improving rumen fermentation and mitigating methane emissions necessitates the use of essential oil blends (EOB) and fumaric acid (FA). This study evaluated the synergistic effect of four EOB with or without FA supplementation on in vitro dry matter digestibility, greenhouse gas emission, and total volatile fatty acid production using inoculum from three rumen-cannulated Black Angus beef cows. The study was arranged in a 4 × 2 + 1 factorial design to evaluate the effects of the four EOB and two FA levels on a total mixed ration (TMR). The EOB dosage was 100 µL while FA was added at 3% of total mixed ration. The EOB × FA interaction (<i>p</i> < 0.05) influenced the dry matter, neutral detergent fiber, and hemicellulose degradabilities. All the EOB and FA (EFA) treatments decreased (<i>p</i> < 0.001) the dry matter degradability compared to the control (TMR substrate only). The EFA4 treatment reduced the neutral detergent fiber and hemicellulose degradabilities compared to the control. The ruminal pH was influenced (<i>p</i> < 0.001) by both the EOB and FA inclusion, and the EOB × FA interaction was significant. The microbial mass was higher (<i>p</i> < 0.001) in the EFA1, EFA4, and EOB4 compared to the control and the EOB3 treatments. The EFA1 and EOB1 produced less (<i>p</i> < 0.001) gas than the control by 29.1 and 32.1%, respectively. Compared with the control, the EFA1 and EOB1 treatments decreased (<i>p</i> < 0.001) methane gas by 90.8% and 86.4%, respectively, while the carbon dioxide was reduced (<i>p</i> = 0.004) by 65.7 and 57.9%, respectively. The EOB × FA interaction was significant (<i>p</i> < 0.001) for the total and individual volatile fatty acid concentrations. The inclusion of FA increased the propionate concentration by 9.5% and decreased (<i>p</i> = 0.02) the acetate concentration by 4%. In summary, the synergistic effect of the EOB and FA offers an effective way to reduce greenhouse gas emission and enhance total volatile fatty acids.https://www.mdpi.com/2673-933X/3/4/31essential oilsfumarateruminantsmethanebatch culture |
| spellingShingle | Joel O. Alabi Deborah O. Okedoyin Chika C. Anotaenwere Michael Wuaku DeAndrea Gray Oludotun O. Adelusi Kelechi A. Ike Lydia K. Olagunju Peter A. Dele Uchenna Y. Anele Essential Oil Blends with or without Fumaric Acid Influenced In Vitro Rumen Fermentation, Greenhouse Gas Emission, and Volatile Fatty Acids Production of a Total Mixed Ration Ruminants essential oils fumarate ruminants methane batch culture |
| title | Essential Oil Blends with or without Fumaric Acid Influenced In Vitro Rumen Fermentation, Greenhouse Gas Emission, and Volatile Fatty Acids Production of a Total Mixed Ration |
| title_full | Essential Oil Blends with or without Fumaric Acid Influenced In Vitro Rumen Fermentation, Greenhouse Gas Emission, and Volatile Fatty Acids Production of a Total Mixed Ration |
| title_fullStr | Essential Oil Blends with or without Fumaric Acid Influenced In Vitro Rumen Fermentation, Greenhouse Gas Emission, and Volatile Fatty Acids Production of a Total Mixed Ration |
| title_full_unstemmed | Essential Oil Blends with or without Fumaric Acid Influenced In Vitro Rumen Fermentation, Greenhouse Gas Emission, and Volatile Fatty Acids Production of a Total Mixed Ration |
| title_short | Essential Oil Blends with or without Fumaric Acid Influenced In Vitro Rumen Fermentation, Greenhouse Gas Emission, and Volatile Fatty Acids Production of a Total Mixed Ration |
| title_sort | essential oil blends with or without fumaric acid influenced in vitro rumen fermentation greenhouse gas emission and volatile fatty acids production of a total mixed ration |
| topic | essential oils fumarate ruminants methane batch culture |
| url | https://www.mdpi.com/2673-933X/3/4/31 |
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