Chronic environmental circadian disruption increases atherosclerosis and dyslipidemia in female, but not male, ApolipoproteinE-deficient mice
Shift work chronically disrupts circadian rhythms and increases the risk of developing cardiovascular disease. However, the mechanisms linking shift work and cardiovascular disease are largely unknown. The goal of this study was to investigate the effects of chronically shifting the light-dark (LD)...
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| Format: | Article |
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2023.1167858/full |
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| author | Jeffrey M. Chalfant Jeffrey M. Chalfant Deborah A. Howatt Victoria B. Johnson Lisa R. Tannock Lisa R. Tannock Lisa R. Tannock Lisa R. Tannock Alan Daugherty Alan Daugherty Julie S. Pendergast Julie S. Pendergast Julie S. Pendergast |
| author_facet | Jeffrey M. Chalfant Jeffrey M. Chalfant Deborah A. Howatt Victoria B. Johnson Lisa R. Tannock Lisa R. Tannock Lisa R. Tannock Lisa R. Tannock Alan Daugherty Alan Daugherty Julie S. Pendergast Julie S. Pendergast Julie S. Pendergast |
| author_sort | Jeffrey M. Chalfant |
| collection | DOAJ |
| description | Shift work chronically disrupts circadian rhythms and increases the risk of developing cardiovascular disease. However, the mechanisms linking shift work and cardiovascular disease are largely unknown. The goal of this study was to investigate the effects of chronically shifting the light-dark (LD) cycle, which models the disordered exposure to light that may occur during shift work, on atherosclerosis. Atherosclerosis is the progressive accumulation of lipid-filled lesions within the artery wall and is the leading cause of cardiovascular disease. We studied ApolipoproteinE-deficient (ApoE−/−) mice that are a well-established model of atherosclerosis. Male and female ApoE−/− mice were housed in control 12L:12D or chronic LD shift conditions for 12 weeks and fed low-fat diet. In the chronic LD shift condition, the light-dark cycle was advanced by 6 h every week. We found that chronic LD shifts exacerbated atherosclerosis in female, but not male, ApoE−/− mice. In females, chronic LD shifts increased total serum cholesterol concentrations with increased atherogenic VLDL/LDL particles. Chronic LD shifts did not affect food intake, activity, or body weight in male or female ApoE−/− mice. We also examined eating behavior in female ApoE−/− mice since aberrant meal timing has been linked to atherosclerosis. The phases of eating behavior rhythms, like locomotor activity rhythms, gradually shifted to the new LD cycle each week in the chronic LD shift group, but there was no effect of the LD shift on the amplitudes of the eating rhythms. Moreover, the duration of fasting intervals was not different in control 12L:12D compared to chronic LD shift conditions. Together these data demonstrate that female ApoE−/− mice have increased atherosclerosis when exposed to chronic LD shifts due to increased VLDL/LDL cholesterol, independent of changes in energy balance or feeding-fasting cycles. |
| first_indexed | 2024-04-09T21:03:59Z |
| format | Article |
| id | doaj.art-55fd1e4ec5f342b6ae656ed535681aca |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-04-09T21:03:59Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Physiology |
| spelling | doaj.art-55fd1e4ec5f342b6ae656ed535681aca2023-03-29T05:05:09ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2023-03-011410.3389/fphys.2023.11678581167858Chronic environmental circadian disruption increases atherosclerosis and dyslipidemia in female, but not male, ApolipoproteinE-deficient miceJeffrey M. Chalfant0Jeffrey M. Chalfant1Deborah A. Howatt2Victoria B. Johnson3Lisa R. Tannock4Lisa R. Tannock5Lisa R. Tannock6Lisa R. Tannock7Alan Daugherty8Alan Daugherty9Julie S. Pendergast10Julie S. Pendergast11Julie S. Pendergast12Department of Biology, University of Kentucky, Lexington, KY, United StatesSaha Cardiovascular Research Center, University of Kentucky, Lexington, KY, United StatesSaha Cardiovascular Research Center, University of Kentucky, Lexington, KY, United StatesDepartment of Biology, University of Kentucky, Lexington, KY, United StatesSaha Cardiovascular Research Center, University of Kentucky, Lexington, KY, United StatesDepartment of Veterans Affairs, Lexington, KY, United StatesDepartment of Internal Medicine, University of Kentucky, Lexington, KY, United StatesBarnstable Brown Diabetes Center, University of Kentucky, Lexington, KY, United StatesSaha Cardiovascular Research Center, University of Kentucky, Lexington, KY, United StatesDepartment of Physiology, University of Kentucky, Lexington, KY, United StatesDepartment of Biology, University of Kentucky, Lexington, KY, United StatesSaha Cardiovascular Research Center, University of Kentucky, Lexington, KY, United StatesBarnstable Brown Diabetes Center, University of Kentucky, Lexington, KY, United StatesShift work chronically disrupts circadian rhythms and increases the risk of developing cardiovascular disease. However, the mechanisms linking shift work and cardiovascular disease are largely unknown. The goal of this study was to investigate the effects of chronically shifting the light-dark (LD) cycle, which models the disordered exposure to light that may occur during shift work, on atherosclerosis. Atherosclerosis is the progressive accumulation of lipid-filled lesions within the artery wall and is the leading cause of cardiovascular disease. We studied ApolipoproteinE-deficient (ApoE−/−) mice that are a well-established model of atherosclerosis. Male and female ApoE−/− mice were housed in control 12L:12D or chronic LD shift conditions for 12 weeks and fed low-fat diet. In the chronic LD shift condition, the light-dark cycle was advanced by 6 h every week. We found that chronic LD shifts exacerbated atherosclerosis in female, but not male, ApoE−/− mice. In females, chronic LD shifts increased total serum cholesterol concentrations with increased atherogenic VLDL/LDL particles. Chronic LD shifts did not affect food intake, activity, or body weight in male or female ApoE−/− mice. We also examined eating behavior in female ApoE−/− mice since aberrant meal timing has been linked to atherosclerosis. The phases of eating behavior rhythms, like locomotor activity rhythms, gradually shifted to the new LD cycle each week in the chronic LD shift group, but there was no effect of the LD shift on the amplitudes of the eating rhythms. Moreover, the duration of fasting intervals was not different in control 12L:12D compared to chronic LD shift conditions. Together these data demonstrate that female ApoE−/− mice have increased atherosclerosis when exposed to chronic LD shifts due to increased VLDL/LDL cholesterol, independent of changes in energy balance or feeding-fasting cycles.https://www.frontiersin.org/articles/10.3389/fphys.2023.1167858/fullcircadian rhythmshift workcardiovascular diseaseeating rhythmfasting |
| spellingShingle | Jeffrey M. Chalfant Jeffrey M. Chalfant Deborah A. Howatt Victoria B. Johnson Lisa R. Tannock Lisa R. Tannock Lisa R. Tannock Lisa R. Tannock Alan Daugherty Alan Daugherty Julie S. Pendergast Julie S. Pendergast Julie S. Pendergast Chronic environmental circadian disruption increases atherosclerosis and dyslipidemia in female, but not male, ApolipoproteinE-deficient mice Frontiers in Physiology circadian rhythm shift work cardiovascular disease eating rhythm fasting |
| title | Chronic environmental circadian disruption increases atherosclerosis and dyslipidemia in female, but not male, ApolipoproteinE-deficient mice |
| title_full | Chronic environmental circadian disruption increases atherosclerosis and dyslipidemia in female, but not male, ApolipoproteinE-deficient mice |
| title_fullStr | Chronic environmental circadian disruption increases atherosclerosis and dyslipidemia in female, but not male, ApolipoproteinE-deficient mice |
| title_full_unstemmed | Chronic environmental circadian disruption increases atherosclerosis and dyslipidemia in female, but not male, ApolipoproteinE-deficient mice |
| title_short | Chronic environmental circadian disruption increases atherosclerosis and dyslipidemia in female, but not male, ApolipoproteinE-deficient mice |
| title_sort | chronic environmental circadian disruption increases atherosclerosis and dyslipidemia in female but not male apolipoproteine deficient mice |
| topic | circadian rhythm shift work cardiovascular disease eating rhythm fasting |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2023.1167858/full |
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