Aconitase and Developmental End Points as Early Indicators of
Background: In this study, the toxicity of the different xenobiotics was tested on the fruit fly Drosophila melanogaster model system. Methods: Fly larvae were raised on food supplemented with xenobiotics at different concentrations (sodium nitroprusside (0.1-1.5 mM), S-nitrosoglutathione (0.5-4 m...
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| Format: | Article |
| Language: | English |
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Arak University of Medical Sciences
2014-03-01
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| Series: | Iranian Journal of Toxicology |
| Subjects: | |
| Online Access: | http://ijt.arakmu.ac.ir/browse.php?a_id=305&sid=1&slc_lang=en |
| _version_ | 1818917098074669056 |
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| author | Oleksandr Vasyliovuch Lozinsky |
| author_facet | Oleksandr Vasyliovuch Lozinsky |
| author_sort | Oleksandr Vasyliovuch Lozinsky |
| collection | DOAJ |
| description | Background: In this study, the toxicity of the different xenobiotics was tested on the fruit fly Drosophila melanogaster model system.
Methods: Fly larvae were raised on food supplemented with xenobiotics at different concentrations (sodium nitroprusside (0.1-1.5 mM), S-nitrosoglutathione (0.5-4 mM), and potassium ferrocyanide (1 mM)). Emergence of flies, food intake by larvae, and pupation height preference as well as aconitase activity (in 2-day old flies) were measured.
Results: Food supplementation with xenobiotics caused a developmental delay in the flies and decreased pupation height. Biochemical analyses of oxidative stress markers and activities of antioxidants and their associated enzymes were carried out on 2-day-old flies emerged from control larvae and larvae fed on food supplemented with chemicals. Larval exposure to chemicals resulted in lower activities of aconitase in flies of both sexes and perturbation in activities of antioxidant enzymes.
Conclusions: The results of this study showed that among a variety of parameters tested, aconitase activity, developmental endpoints, and pupation height may be used as reliable early indicators of toxicity caused by different chemicals. |
| first_indexed | 2024-12-20T00:28:40Z |
| format | Article |
| id | doaj.art-55fe0367350f4610adf50520da9fc9e1 |
| institution | Directory Open Access Journal |
| issn | 2008-2967 2251-9459 |
| language | English |
| last_indexed | 2024-12-20T00:28:40Z |
| publishDate | 2014-03-01 |
| publisher | Arak University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Toxicology |
| spelling | doaj.art-55fe0367350f4610adf50520da9fc9e12022-12-21T20:00:00ZengArak University of Medical SciencesIranian Journal of Toxicology2008-29672251-94592014-03-018249981003Aconitase and Developmental End Points as Early Indicators ofOleksandr Vasyliovuch Lozinsky 0Department of Biochemistry and Biotechnology, Precarpathian National University (Vassyl Stefanyk), Ivano-Frankivsk, Ukraine. E-mail: lozinsky_o@ukr.netBackground: In this study, the toxicity of the different xenobiotics was tested on the fruit fly Drosophila melanogaster model system. Methods: Fly larvae were raised on food supplemented with xenobiotics at different concentrations (sodium nitroprusside (0.1-1.5 mM), S-nitrosoglutathione (0.5-4 mM), and potassium ferrocyanide (1 mM)). Emergence of flies, food intake by larvae, and pupation height preference as well as aconitase activity (in 2-day old flies) were measured. Results: Food supplementation with xenobiotics caused a developmental delay in the flies and decreased pupation height. Biochemical analyses of oxidative stress markers and activities of antioxidants and their associated enzymes were carried out on 2-day-old flies emerged from control larvae and larvae fed on food supplemented with chemicals. Larval exposure to chemicals resulted in lower activities of aconitase in flies of both sexes and perturbation in activities of antioxidant enzymes. Conclusions: The results of this study showed that among a variety of parameters tested, aconitase activity, developmental endpoints, and pupation height may be used as reliable early indicators of toxicity caused by different chemicals.http://ijt.arakmu.ac.ir/browse.php?a_id=305&sid=1&slc_lang=enAconitate HydrataseDrosophila MelanogasterOxidative Stress |
| spellingShingle | Oleksandr Vasyliovuch Lozinsky Aconitase and Developmental End Points as Early Indicators of Iranian Journal of Toxicology Aconitate Hydratase Drosophila Melanogaster Oxidative Stress |
| title | Aconitase and Developmental End Points as Early Indicators of |
| title_full | Aconitase and Developmental End Points as Early Indicators of |
| title_fullStr | Aconitase and Developmental End Points as Early Indicators of |
| title_full_unstemmed | Aconitase and Developmental End Points as Early Indicators of |
| title_short | Aconitase and Developmental End Points as Early Indicators of |
| title_sort | aconitase and developmental end points as early indicators of |
| topic | Aconitate Hydratase Drosophila Melanogaster Oxidative Stress |
| url | http://ijt.arakmu.ac.ir/browse.php?a_id=305&sid=1&slc_lang=en |
| work_keys_str_mv | AT oleksandrvasyliovuchlozinsky aconitaseanddevelopmentalendpointsasearlyindicatorsof |