Nitrogen-Activated CLV3/ESR-Related 4 (CLE4) Regulates Shoot, Root, and Stolon Growth in Potato
In potato, high levels of nitrogen (N) can lead to excessive vegetative growth at the expense of tuber development, resulting in lower yield and poor-quality tubers. We found that <i>Solanum tuberosum CLE4</i> (<i>StCLE4</i>) is expressed most strongly in the roots grown in N...
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MDPI AG
2023-10-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/19/3468 |
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| author | Maria S. Gancheva Lyudmila A. Lutova |
| author_facet | Maria S. Gancheva Lyudmila A. Lutova |
| author_sort | Maria S. Gancheva |
| collection | DOAJ |
| description | In potato, high levels of nitrogen (N) can lead to excessive vegetative growth at the expense of tuber development, resulting in lower yield and poor-quality tubers. We found that <i>Solanum tuberosum CLE4</i> (<i>StCLE4</i>) is expressed most strongly in the roots grown in N-rich media, and it positively regulates potato root growth under N-deficient conditions. We noted that StCLE4 functions as a negative regulator of normal shoot apex development similar to CLV3 in Arabidopsis. Transcriptomic analysis revealed that overexpression of <i>StCLE4</i> resulted in the repression of the <i>StIT1</i> gene, a regulator of potato tuber initiation. <i>StCLE4</i>-overexpressing stolons were converted into branches, that were similar to a mild phenotype of the <i>it1</i> (<i>identity of tuber 1</i>) mutant. We also found that NIN-like proteins, key regulators of nitrate signaling bind to the regulatory sequence of <i>StIT1</i> in a yeast one-hybrid assay. Taken together, our findings suggest that StCLE4 regulates shoot, root, and stolon growth in potato. |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T21:37:39Z |
| publishDate | 2023-10-01 |
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| spelling | doaj.art-55ffe945c85e45c088cd5f4e969721de2023-11-19T14:54:49ZengMDPI AGPlants2223-77472023-10-011219346810.3390/plants12193468Nitrogen-Activated CLV3/ESR-Related 4 (CLE4) Regulates Shoot, Root, and Stolon Growth in PotatoMaria S. Gancheva0Lyudmila A. Lutova1Department of Genetics and Biotechnology, Saint Petersburg State University, Universitetskaya emb. 7/9, Saint Petersburg 199034, RussiaDepartment of Genetics and Biotechnology, Saint Petersburg State University, Universitetskaya emb. 7/9, Saint Petersburg 199034, RussiaIn potato, high levels of nitrogen (N) can lead to excessive vegetative growth at the expense of tuber development, resulting in lower yield and poor-quality tubers. We found that <i>Solanum tuberosum CLE4</i> (<i>StCLE4</i>) is expressed most strongly in the roots grown in N-rich media, and it positively regulates potato root growth under N-deficient conditions. We noted that StCLE4 functions as a negative regulator of normal shoot apex development similar to CLV3 in Arabidopsis. Transcriptomic analysis revealed that overexpression of <i>StCLE4</i> resulted in the repression of the <i>StIT1</i> gene, a regulator of potato tuber initiation. <i>StCLE4</i>-overexpressing stolons were converted into branches, that were similar to a mild phenotype of the <i>it1</i> (<i>identity of tuber 1</i>) mutant. We also found that NIN-like proteins, key regulators of nitrate signaling bind to the regulatory sequence of <i>StIT1</i> in a yeast one-hybrid assay. Taken together, our findings suggest that StCLE4 regulates shoot, root, and stolon growth in potato.https://www.mdpi.com/2223-7747/12/19/3468potatoCLE peptideCLE4NIN-like proteins (NLPs)nitratenitrate-responsive elements (NREs) |
| spellingShingle | Maria S. Gancheva Lyudmila A. Lutova Nitrogen-Activated CLV3/ESR-Related 4 (CLE4) Regulates Shoot, Root, and Stolon Growth in Potato Plants potato CLE peptide CLE4 NIN-like proteins (NLPs) nitrate nitrate-responsive elements (NREs) |
| title | Nitrogen-Activated CLV3/ESR-Related 4 (CLE4) Regulates Shoot, Root, and Stolon Growth in Potato |
| title_full | Nitrogen-Activated CLV3/ESR-Related 4 (CLE4) Regulates Shoot, Root, and Stolon Growth in Potato |
| title_fullStr | Nitrogen-Activated CLV3/ESR-Related 4 (CLE4) Regulates Shoot, Root, and Stolon Growth in Potato |
| title_full_unstemmed | Nitrogen-Activated CLV3/ESR-Related 4 (CLE4) Regulates Shoot, Root, and Stolon Growth in Potato |
| title_short | Nitrogen-Activated CLV3/ESR-Related 4 (CLE4) Regulates Shoot, Root, and Stolon Growth in Potato |
| title_sort | nitrogen activated clv3 esr related 4 cle4 regulates shoot root and stolon growth in potato |
| topic | potato CLE peptide CLE4 NIN-like proteins (NLPs) nitrate nitrate-responsive elements (NREs) |
| url | https://www.mdpi.com/2223-7747/12/19/3468 |
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