Ascorbate Oxidase-Based Amperometric Biosensor for L-Ascorbic Acid Determination in Beverages
A novel biosensor for l-ascorbic acid determination in different beverages was elaborated. The ascorbate oxidase enzyme (AAO) from Cucurbita sp., EC 1.10.3.3, was immobilized on a screen-printed carbon electrode with poly(ethylene glycol) (400) diglycidyl ether (PEGDGE) as a crosslinking agent. The...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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University of Zagreb Faculty of Food Technology and Biotechnology
2016-01-01
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| Series: | Food Technology and Biotechnology |
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| Online Access: | https://hrcak.srce.hr/file/228597 |
| _version_ | 1827283211545214976 |
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| author | Gábor Csiffáry Péter Fűtő Nóra Adányi Attila Kiss |
| author_facet | Gábor Csiffáry Péter Fűtő Nóra Adányi Attila Kiss |
| author_sort | Gábor Csiffáry |
| collection | DOAJ |
| description | A novel biosensor for l-ascorbic acid determination in different beverages was elaborated. The ascorbate oxidase enzyme (AAO) from Cucurbita sp., EC 1.10.3.3, was immobilized on a screen-printed carbon electrode with poly(ethylene glycol) (400) diglycidyl ether (PEGDGE) as a crosslinking agent. The standards and samples were measured first with a blank electrode. An inert protein, bovine serum albumin (BSA), was immobilized on the surface of this electrode with PEGDGE. The BSA mass was equivalent to the mass of 10 U of AAO enzyme immobilized on the electrodes (0.021 mg). The linear measuring range for l-ascorbic acid was between 5 and 150 μmol/L. As l-ascorbic acid is a vital vitamin and a common antioxidant used in food industry, fruit juices and vitamin C effervescent tablets were examined. The results were compared to HPLC measurements. |
| first_indexed | 2024-04-24T09:31:56Z |
| format | Article |
| id | doaj.art-560a32ab16954a7a8b2bba4a695a04b2 |
| institution | Directory Open Access Journal |
| issn | 1330-9862 1334-2606 |
| language | English |
| last_indexed | 2024-04-24T09:31:56Z |
| publishDate | 2016-01-01 |
| publisher | University of Zagreb Faculty of Food Technology and Biotechnology |
| record_format | Article |
| series | Food Technology and Biotechnology |
| spelling | doaj.art-560a32ab16954a7a8b2bba4a695a04b22024-04-15T13:48:14ZengUniversity of Zagreb Faculty of Food Technology and BiotechnologyFood Technology and Biotechnology1330-98621334-26062016-01-01541313510.17113/ftb.54.01.16.4135Ascorbate Oxidase-Based Amperometric Biosensor for L-Ascorbic Acid Determination in BeveragesGábor Csiffáry0Péter Fűtő1Nóra Adányi2Attila Kiss3Eszterházy Károly College, EGERFOOD Knowledge Centre, Eger, HungaryEszterházy Károly College, EGERFOOD Knowledge Centre, Eger, HungaryNational Agricultural Research and Innovation Centre, Food Science Research Institute, Budapest, HungaryNational Agricultural Research and Innovation Centre, Food Science Research Institute, Budapest, HungaryA novel biosensor for l-ascorbic acid determination in different beverages was elaborated. The ascorbate oxidase enzyme (AAO) from Cucurbita sp., EC 1.10.3.3, was immobilized on a screen-printed carbon electrode with poly(ethylene glycol) (400) diglycidyl ether (PEGDGE) as a crosslinking agent. The standards and samples were measured first with a blank electrode. An inert protein, bovine serum albumin (BSA), was immobilized on the surface of this electrode with PEGDGE. The BSA mass was equivalent to the mass of 10 U of AAO enzyme immobilized on the electrodes (0.021 mg). The linear measuring range for l-ascorbic acid was between 5 and 150 μmol/L. As l-ascorbic acid is a vital vitamin and a common antioxidant used in food industry, fruit juices and vitamin C effervescent tablets were examined. The results were compared to HPLC measurements.https://hrcak.srce.hr/file/228597biosensorl-ascorbic acidascorbate oxidasescreen-printed carbon electrodes |
| spellingShingle | Gábor Csiffáry Péter Fűtő Nóra Adányi Attila Kiss Ascorbate Oxidase-Based Amperometric Biosensor for L-Ascorbic Acid Determination in Beverages Food Technology and Biotechnology biosensor l-ascorbic acid ascorbate oxidase screen-printed carbon electrodes |
| title | Ascorbate Oxidase-Based Amperometric Biosensor for L-Ascorbic Acid Determination in Beverages |
| title_full | Ascorbate Oxidase-Based Amperometric Biosensor for L-Ascorbic Acid Determination in Beverages |
| title_fullStr | Ascorbate Oxidase-Based Amperometric Biosensor for L-Ascorbic Acid Determination in Beverages |
| title_full_unstemmed | Ascorbate Oxidase-Based Amperometric Biosensor for L-Ascorbic Acid Determination in Beverages |
| title_short | Ascorbate Oxidase-Based Amperometric Biosensor for L-Ascorbic Acid Determination in Beverages |
| title_sort | ascorbate oxidase based amperometric biosensor for l ascorbic acid determination in beverages |
| topic | biosensor l-ascorbic acid ascorbate oxidase screen-printed carbon electrodes |
| url | https://hrcak.srce.hr/file/228597 |
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