Prevalence and Mycotoxigenic Potential of Fungi in Fish Feed Collected from Fish Farms in Egypt with a Particular Reference to Aflatoxins Contamination
The current study is aimed to investigate the fungal contaminants in fish feed. Isolation of fungi was conducted on modified dichloran 18% glycerol agar (DG18) and potato dextrose agar (PDA). Feed samples were assayed for aflatoxins using HPLC. A total of 43 species belonging to 19 fungal gene...
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| Format: | Article |
| Language: | English |
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Assiut University
2024-01-01
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| Series: | Journal of Advanced Veterinary Research |
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| Online Access: | https://advetresearch.com/index.php/AVR/article/view/1678 |
| _version_ | 1797342304333201408 |
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| author | Yasmen Galal Magdy El-Bana Mohamed M. Deabes Abdel-Hamied M. Rasmey |
| author_facet | Yasmen Galal Magdy El-Bana Mohamed M. Deabes Abdel-Hamied M. Rasmey |
| author_sort | Yasmen Galal |
| collection | DOAJ |
| description |
The current study is aimed to investigate the fungal contaminants in fish feed. Isolation of fungi was conducted on modified dichloran 18% glycerol agar (DG18) and potato dextrose agar (PDA). Feed samples were assayed for aflatoxins using HPLC. A total of 43 species belonging to 19 fungal genera recovered from 45 fish feed samples. Aspergillus and Penicillium were the most predominant genera with isolation frequency values indicated the retrieval capability of DG18 over PDA medium. For instance, Aspergillus spp. recorded 60%, 53.3% while Penicillium spp. were 33.3%, 17.8% on DG18 and PDA respectively via direct plating. 41.4% of the tested isolates were mycotoxin producers. Aflatoxins B1, B2, G1 and G2 were detected by 6 out of 10 screened Aspergillus isolates. Fumitremorgens, Gliotoxin, Ochratoxin A and B, and Zeralenone were also detected. The feed samples of high total count percentages (TC%) of A. flavus recorded the highest incidence of aflatoxins B2, G1 and G2 (2.3, 35.3 and 7.8 ng/g respectively). Meanwhile, the highest B1 concentration (3.7 ng/g) was recorded for the highest TC% interval studied (1:9 cfu/g). Thus, it is important to monitor the fungal load and mycotoxins in fish feed periodically using proper practical approaches.
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| first_indexed | 2024-03-08T10:31:20Z |
| format | Article |
| id | doaj.art-560ca5d492984db087ee32a20bd1a444 |
| institution | Directory Open Access Journal |
| issn | 2090-6269 2090-6277 |
| language | English |
| last_indexed | 2024-03-08T10:31:20Z |
| publishDate | 2024-01-01 |
| publisher | Assiut University |
| record_format | Article |
| series | Journal of Advanced Veterinary Research |
| spelling | doaj.art-560ca5d492984db087ee32a20bd1a4442024-01-27T04:50:47ZengAssiut UniversityJournal of Advanced Veterinary Research2090-62692090-62772024-01-011310Prevalence and Mycotoxigenic Potential of Fungi in Fish Feed Collected from Fish Farms in Egypt with a Particular Reference to Aflatoxins ContaminationYasmen Galal0Magdy El-Bana1Mohamed M. Deabes2Abdel-Hamied M. Rasmey3Botany and Microbiology Department, Faculty of Science, Port-Said University, Port Said, Egypt.Botany and Microbiology Department, Faculty of Science, Port-Said University, Port Said, Egypt.Food Toxicology and Contaminants Department, Food Industry and Nutrition Division, National Research Centre, Giza, Egypt.Botany and Microbiology Department, Faculty of Science, Suez University, Suez, Egypt. The current study is aimed to investigate the fungal contaminants in fish feed. Isolation of fungi was conducted on modified dichloran 18% glycerol agar (DG18) and potato dextrose agar (PDA). Feed samples were assayed for aflatoxins using HPLC. A total of 43 species belonging to 19 fungal genera recovered from 45 fish feed samples. Aspergillus and Penicillium were the most predominant genera with isolation frequency values indicated the retrieval capability of DG18 over PDA medium. For instance, Aspergillus spp. recorded 60%, 53.3% while Penicillium spp. were 33.3%, 17.8% on DG18 and PDA respectively via direct plating. 41.4% of the tested isolates were mycotoxin producers. Aflatoxins B1, B2, G1 and G2 were detected by 6 out of 10 screened Aspergillus isolates. Fumitremorgens, Gliotoxin, Ochratoxin A and B, and Zeralenone were also detected. The feed samples of high total count percentages (TC%) of A. flavus recorded the highest incidence of aflatoxins B2, G1 and G2 (2.3, 35.3 and 7.8 ng/g respectively). Meanwhile, the highest B1 concentration (3.7 ng/g) was recorded for the highest TC% interval studied (1:9 cfu/g). Thus, it is important to monitor the fungal load and mycotoxins in fish feed periodically using proper practical approaches. https://advetresearch.com/index.php/AVR/article/view/1678 AflatoxinsAspergillus flavusContaminationFish feedHPLC |
| spellingShingle | Yasmen Galal Magdy El-Bana Mohamed M. Deabes Abdel-Hamied M. Rasmey Prevalence and Mycotoxigenic Potential of Fungi in Fish Feed Collected from Fish Farms in Egypt with a Particular Reference to Aflatoxins Contamination Journal of Advanced Veterinary Research Aflatoxins Aspergillus flavus Contamination Fish feed HPLC |
| title | Prevalence and Mycotoxigenic Potential of Fungi in Fish Feed Collected from Fish Farms in Egypt with a Particular Reference to Aflatoxins Contamination |
| title_full | Prevalence and Mycotoxigenic Potential of Fungi in Fish Feed Collected from Fish Farms in Egypt with a Particular Reference to Aflatoxins Contamination |
| title_fullStr | Prevalence and Mycotoxigenic Potential of Fungi in Fish Feed Collected from Fish Farms in Egypt with a Particular Reference to Aflatoxins Contamination |
| title_full_unstemmed | Prevalence and Mycotoxigenic Potential of Fungi in Fish Feed Collected from Fish Farms in Egypt with a Particular Reference to Aflatoxins Contamination |
| title_short | Prevalence and Mycotoxigenic Potential of Fungi in Fish Feed Collected from Fish Farms in Egypt with a Particular Reference to Aflatoxins Contamination |
| title_sort | prevalence and mycotoxigenic potential of fungi in fish feed collected from fish farms in egypt with a particular reference to aflatoxins contamination |
| topic | Aflatoxins Aspergillus flavus Contamination Fish feed HPLC |
| url | https://advetresearch.com/index.php/AVR/article/view/1678 |
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