Identification of Neuronal Cells in Sciatic Nerves of Adult Rats
Prior research generally confirms that there are no neuronal cell bodies in the adult sciatic nerve. However, we occasionally find some neuronal cells in adult rat sciatic nerves, either intact or crush-injured. By whole-mount staining and optical imaging of the hyalinized sciatic nerves for Stmn2 (...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-03-01
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| Series: | Frontiers in Cellular Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fncel.2022.816814/full |
| _version_ | 1828313363278462976 |
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| author | Yisheng Liu Songlin Zhou Lili Zhao Xiaosong Gu Xiaosong Gu |
| author_facet | Yisheng Liu Songlin Zhou Lili Zhao Xiaosong Gu Xiaosong Gu |
| author_sort | Yisheng Liu |
| collection | DOAJ |
| description | Prior research generally confirms that there are no neuronal cell bodies in the adult sciatic nerve. However, we occasionally find some neuronal cells in adult rat sciatic nerves, either intact or crush-injured. By whole-mount staining and optical imaging of the hyalinized sciatic nerves for Stmn2 (a specific marker for neuronal cells), we found those neuronal cells with irregular distribution in the sciatic nerves in both crushed model and normal rats. We investigated the identity of those cells and established a cultured sciatic nerve model. Immunohistochemistry evidence both in vivo and in vitro illustrated that some of those cells are mature neurons in sciatic nerves. With single-cell sequencing of neuronal cells in adeno-associated virus (AAV)-infected sciatic nerves, we identified that some of those cells are a kind of neuronal stem-like cells. Then we constructed a Nestin-CreERT2 rat line and traced those cells with fluorescence labeling which was induced by tamoxifen. Interesting, we proved that neuronal stem-like cells could proliferate by combination of EdU incorporation with staining in the sciatic nerves of transgenic rats. Together, the discovery of neuronal cells in adult sciatic nerves will make us aware of the distribution of neurons in the peripheral nervous system. Especially our data suggest that neuronal stem-like cells could proliferate in the sciatic nerves of adult rats. |
| first_indexed | 2024-04-13T16:29:36Z |
| format | Article |
| id | doaj.art-560cac822ea841b981c4f1d6dfee1896 |
| institution | Directory Open Access Journal |
| issn | 1662-5102 |
| language | English |
| last_indexed | 2024-04-13T16:29:36Z |
| publishDate | 2022-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular Neuroscience |
| spelling | doaj.art-560cac822ea841b981c4f1d6dfee18962022-12-22T02:39:37ZengFrontiers Media S.A.Frontiers in Cellular Neuroscience1662-51022022-03-011610.3389/fncel.2022.816814816814Identification of Neuronal Cells in Sciatic Nerves of Adult RatsYisheng Liu0Songlin Zhou1Lili Zhao2Xiaosong Gu3Xiaosong Gu4Model Animal Research Center, Nanjing University, Nanjing, ChinaKey Laboratory of Neuroregeneration, Ministry of Education and Jiangsu Province, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, ChinaModel Animal Research Center, Nanjing University, Nanjing, ChinaModel Animal Research Center, Nanjing University, Nanjing, ChinaKey Laboratory of Neuroregeneration, Ministry of Education and Jiangsu Province, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, ChinaPrior research generally confirms that there are no neuronal cell bodies in the adult sciatic nerve. However, we occasionally find some neuronal cells in adult rat sciatic nerves, either intact or crush-injured. By whole-mount staining and optical imaging of the hyalinized sciatic nerves for Stmn2 (a specific marker for neuronal cells), we found those neuronal cells with irregular distribution in the sciatic nerves in both crushed model and normal rats. We investigated the identity of those cells and established a cultured sciatic nerve model. Immunohistochemistry evidence both in vivo and in vitro illustrated that some of those cells are mature neurons in sciatic nerves. With single-cell sequencing of neuronal cells in adeno-associated virus (AAV)-infected sciatic nerves, we identified that some of those cells are a kind of neuronal stem-like cells. Then we constructed a Nestin-CreERT2 rat line and traced those cells with fluorescence labeling which was induced by tamoxifen. Interesting, we proved that neuronal stem-like cells could proliferate by combination of EdU incorporation with staining in the sciatic nerves of transgenic rats. Together, the discovery of neuronal cells in adult sciatic nerves will make us aware of the distribution of neurons in the peripheral nervous system. Especially our data suggest that neuronal stem-like cells could proliferate in the sciatic nerves of adult rats.https://www.frontiersin.org/articles/10.3389/fncel.2022.816814/fullneuronal cell bodiestransgenic rat modelcrushed sciatic nervesciatic nerve culturesingle-cell sequencingneuronal stem-like cells |
| spellingShingle | Yisheng Liu Songlin Zhou Lili Zhao Xiaosong Gu Xiaosong Gu Identification of Neuronal Cells in Sciatic Nerves of Adult Rats Frontiers in Cellular Neuroscience neuronal cell bodies transgenic rat model crushed sciatic nerve sciatic nerve culture single-cell sequencing neuronal stem-like cells |
| title | Identification of Neuronal Cells in Sciatic Nerves of Adult Rats |
| title_full | Identification of Neuronal Cells in Sciatic Nerves of Adult Rats |
| title_fullStr | Identification of Neuronal Cells in Sciatic Nerves of Adult Rats |
| title_full_unstemmed | Identification of Neuronal Cells in Sciatic Nerves of Adult Rats |
| title_short | Identification of Neuronal Cells in Sciatic Nerves of Adult Rats |
| title_sort | identification of neuronal cells in sciatic nerves of adult rats |
| topic | neuronal cell bodies transgenic rat model crushed sciatic nerve sciatic nerve culture single-cell sequencing neuronal stem-like cells |
| url | https://www.frontiersin.org/articles/10.3389/fncel.2022.816814/full |
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