Resource: A Cellular Developmental Taxonomy of the Bone Marrow Mesenchymal Stem Cell Population in Mice
Mesenchymal stem cells (MSCs) play pivotal roles in tissue (re)generation. In the murine bone marrow, they are thought to reside within the Sca-1+ CD51+ bone marrow stromal cell population. Here, using scRNAseq, we aimed to delineate the cellularheterogeneity of this MSC-enriched population througho...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-02-01
|
| Series: | HemaSphere |
| Online Access: | http://journals.lww.com/10.1097/HS9.0000000000000823 |
| _version_ | 1797283162121830400 |
|---|---|
| author | Paola Pisterzi Lanpeng Chen Claire van Dijk Michiel J. W. Wevers Eric J. M. Bindels Marc H. G. P. Raaijmakers |
| author_facet | Paola Pisterzi Lanpeng Chen Claire van Dijk Michiel J. W. Wevers Eric J. M. Bindels Marc H. G. P. Raaijmakers |
| author_sort | Paola Pisterzi |
| collection | DOAJ |
| description | Mesenchymal stem cells (MSCs) play pivotal roles in tissue (re)generation. In the murine bone marrow, they are thought to reside within the Sca-1+ CD51+ bone marrow stromal cell population. Here, using scRNAseq, we aimed to delineate the cellularheterogeneity of this MSC-enriched population throughout development. At the fetal stage, the MSC population is relatively homogeneous with subsets predicted to contain stem/progenitor cells, based on transcriptional modeling and marker expression. These subsets decline in relative size throughout life, with postnatal emergence of specialized clusters, including hematopoietic stem/progenitor cell (HSPC) niches. In fetal development, these stromal HSPC niches are lacking, but subsets of endothelial cells express HSPC factors, suggesting that they may provide initial niches for emerging hematopoiesis. This cellular taxonomy of the MSC population upon development is anticipated to provide a resource aiding the prospective identification of cellular subsets and molecular mechanisms driving bone marrow (re)generation. |
| first_indexed | 2024-03-07T17:27:05Z |
| format | Article |
| id | doaj.art-560dd67d94af45b2951c89961956b393 |
| institution | Directory Open Access Journal |
| issn | 2572-9241 |
| language | English |
| last_indexed | 2024-03-07T17:27:05Z |
| publishDate | 2023-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | HemaSphere |
| spelling | doaj.art-560dd67d94af45b2951c89961956b3932024-03-02T18:49:40ZengWileyHemaSphere2572-92412023-02-0172e82310.1097/HS9.0000000000000823202302000-00010Resource: A Cellular Developmental Taxonomy of the Bone Marrow Mesenchymal Stem Cell Population in MicePaola Pisterzi0Lanpeng Chen1Claire van Dijk2Michiel J. W. Wevers3Eric J. M. Bindels4Marc H. G. P. Raaijmakers51 Department of Hematology, Erasmus MC Cancer Institute, Rotterdam, Netherlands1 Department of Hematology, Erasmus MC Cancer Institute, Rotterdam, Netherlands1 Department of Hematology, Erasmus MC Cancer Institute, Rotterdam, Netherlands1 Department of Hematology, Erasmus MC Cancer Institute, Rotterdam, Netherlands1 Department of Hematology, Erasmus MC Cancer Institute, Rotterdam, Netherlands1 Department of Hematology, Erasmus MC Cancer Institute, Rotterdam, NetherlandsMesenchymal stem cells (MSCs) play pivotal roles in tissue (re)generation. In the murine bone marrow, they are thought to reside within the Sca-1+ CD51+ bone marrow stromal cell population. Here, using scRNAseq, we aimed to delineate the cellularheterogeneity of this MSC-enriched population throughout development. At the fetal stage, the MSC population is relatively homogeneous with subsets predicted to contain stem/progenitor cells, based on transcriptional modeling and marker expression. These subsets decline in relative size throughout life, with postnatal emergence of specialized clusters, including hematopoietic stem/progenitor cell (HSPC) niches. In fetal development, these stromal HSPC niches are lacking, but subsets of endothelial cells express HSPC factors, suggesting that they may provide initial niches for emerging hematopoiesis. This cellular taxonomy of the MSC population upon development is anticipated to provide a resource aiding the prospective identification of cellular subsets and molecular mechanisms driving bone marrow (re)generation.http://journals.lww.com/10.1097/HS9.0000000000000823 |
| spellingShingle | Paola Pisterzi Lanpeng Chen Claire van Dijk Michiel J. W. Wevers Eric J. M. Bindels Marc H. G. P. Raaijmakers Resource: A Cellular Developmental Taxonomy of the Bone Marrow Mesenchymal Stem Cell Population in Mice HemaSphere |
| title | Resource: A Cellular Developmental Taxonomy of the Bone Marrow Mesenchymal Stem Cell Population in Mice |
| title_full | Resource: A Cellular Developmental Taxonomy of the Bone Marrow Mesenchymal Stem Cell Population in Mice |
| title_fullStr | Resource: A Cellular Developmental Taxonomy of the Bone Marrow Mesenchymal Stem Cell Population in Mice |
| title_full_unstemmed | Resource: A Cellular Developmental Taxonomy of the Bone Marrow Mesenchymal Stem Cell Population in Mice |
| title_short | Resource: A Cellular Developmental Taxonomy of the Bone Marrow Mesenchymal Stem Cell Population in Mice |
| title_sort | resource a cellular developmental taxonomy of the bone marrow mesenchymal stem cell population in mice |
| url | http://journals.lww.com/10.1097/HS9.0000000000000823 |
| work_keys_str_mv | AT paolapisterzi resourceacellulardevelopmentaltaxonomyofthebonemarrowmesenchymalstemcellpopulationinmice AT lanpengchen resourceacellulardevelopmentaltaxonomyofthebonemarrowmesenchymalstemcellpopulationinmice AT clairevandijk resourceacellulardevelopmentaltaxonomyofthebonemarrowmesenchymalstemcellpopulationinmice AT michieljwwevers resourceacellulardevelopmentaltaxonomyofthebonemarrowmesenchymalstemcellpopulationinmice AT ericjmbindels resourceacellulardevelopmentaltaxonomyofthebonemarrowmesenchymalstemcellpopulationinmice AT marchgpraaijmakers resourceacellulardevelopmentaltaxonomyofthebonemarrowmesenchymalstemcellpopulationinmice |