Surface modification of Polydimethylsiloxane by hydrogels for microfluidic applications
In vitro, hydrogel-based ECMs for functionalizing surfaces of various material have played an essential role in mimicking native tissue matrix. Polydimethylsiloxane (PDMS) is widely used to build microfluidic or organ-on-chip devices compatible with cells due to its easy handling in cast replication...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
De Gruyter
2019-09-01
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| Series: | Current Directions in Biomedical Engineering |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/cdbme-2019-0024 |
| _version_ | 1797989293472350208 |
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| author | Kemkemer Ralf Zenghao Zhang Linxiao Yang Athanasopulu Kiriaki Frey Kerstin Cui Zhishan Su Haijia Luo Liu |
| author_facet | Kemkemer Ralf Zenghao Zhang Linxiao Yang Athanasopulu Kiriaki Frey Kerstin Cui Zhishan Su Haijia Luo Liu |
| author_sort | Kemkemer Ralf |
| collection | DOAJ |
| description | In vitro, hydrogel-based ECMs for functionalizing surfaces of various material have played an essential role in mimicking native tissue matrix. Polydimethylsiloxane (PDMS) is widely used to build microfluidic or organ-on-chip devices compatible with cells due to its easy handling in cast replication. Despite such advantages, the limitation of PDMS is its hydrophobic surface property. To improve wettability of PDMS-based devices, alginate, a naturally derived polysaccharide, was covalently bound to the PDMS surface. This alginate then crosslinked further hydrogel onto the PDMS surface in desired layer thickness. Hydrogel-modified PDMS was used for coating a topography chip system and in vitro investigation of cell growth on the surfaces. Moreover, such hydrophilic hydrogel-coated PDMS is utilized in a microfluidic device to prevent unspecific absorption of organic solutions. Hence, in both exemplary studies, PDMS surface properties were modified leading to improved devices. |
| first_indexed | 2024-04-11T08:18:00Z |
| format | Article |
| id | doaj.art-560eb013f9884243a6a08e54ee43f204 |
| institution | Directory Open Access Journal |
| issn | 2364-5504 |
| language | English |
| last_indexed | 2024-04-11T08:18:00Z |
| publishDate | 2019-09-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Current Directions in Biomedical Engineering |
| spelling | doaj.art-560eb013f9884243a6a08e54ee43f2042022-12-22T04:35:04ZengDe GruyterCurrent Directions in Biomedical Engineering2364-55042019-09-0151939610.1515/cdbme-2019-0024cdbme-2019-0024Surface modification of Polydimethylsiloxane by hydrogels for microfluidic applicationsKemkemer Ralf0Zenghao Zhang1Linxiao Yang2Athanasopulu Kiriaki3Frey Kerstin4Cui Zhishan5Su Haijia6Luo Liu7Applied Chemistry, Reutlingen University,Reutlingen, GermanyBeijing Bioprocess Key Laboratory, Beijing University of Chemical Technology,Beijing, PR ChinaReutlingen Research Institute, Reutlingen University,Reutlingen, GermanyApplied Chemistry, Reutlingen University,Reutlingen, GermanyApplied Chemistry, Reutlingen University,Reutlingen, GermanyApplied Chemistry, Reutlingen University,Reutlingen, GermanyBeijing Bioprocess Key Laboratory, Beijing University of Chemical Technology,Beijing, PR ChinaBeijing Bioprocess Key Laboratory, Beijing University of Chemical Technology,Beijing, PR ChinaIn vitro, hydrogel-based ECMs for functionalizing surfaces of various material have played an essential role in mimicking native tissue matrix. Polydimethylsiloxane (PDMS) is widely used to build microfluidic or organ-on-chip devices compatible with cells due to its easy handling in cast replication. Despite such advantages, the limitation of PDMS is its hydrophobic surface property. To improve wettability of PDMS-based devices, alginate, a naturally derived polysaccharide, was covalently bound to the PDMS surface. This alginate then crosslinked further hydrogel onto the PDMS surface in desired layer thickness. Hydrogel-modified PDMS was used for coating a topography chip system and in vitro investigation of cell growth on the surfaces. Moreover, such hydrophilic hydrogel-coated PDMS is utilized in a microfluidic device to prevent unspecific absorption of organic solutions. Hence, in both exemplary studies, PDMS surface properties were modified leading to improved devices.https://doi.org/10.1515/cdbme-2019-0024pdmsalginatehydrogelmicrofluidic chipscell culture |
| spellingShingle | Kemkemer Ralf Zenghao Zhang Linxiao Yang Athanasopulu Kiriaki Frey Kerstin Cui Zhishan Su Haijia Luo Liu Surface modification of Polydimethylsiloxane by hydrogels for microfluidic applications Current Directions in Biomedical Engineering pdms alginate hydrogel microfluidic chips cell culture |
| title | Surface modification of Polydimethylsiloxane by hydrogels for microfluidic applications |
| title_full | Surface modification of Polydimethylsiloxane by hydrogels for microfluidic applications |
| title_fullStr | Surface modification of Polydimethylsiloxane by hydrogels for microfluidic applications |
| title_full_unstemmed | Surface modification of Polydimethylsiloxane by hydrogels for microfluidic applications |
| title_short | Surface modification of Polydimethylsiloxane by hydrogels for microfluidic applications |
| title_sort | surface modification of polydimethylsiloxane by hydrogels for microfluidic applications |
| topic | pdms alginate hydrogel microfluidic chips cell culture |
| url | https://doi.org/10.1515/cdbme-2019-0024 |
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