Effect of miR-216a on macrophages derived from human peripheral blood mononuclear cells
Objective To study the role of miR-216a in regulating the characteristics of macrophages derived from peripheral blood mononuclear cells (PBMCs). Methods Human PBMCs were isolated and cultured. The polarization model of M1 and M2 macrophages were established in vitro, and flow cytometry and real-tim...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2022-03-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2022-42-3-384.pdf |
| _version_ | 1827390647584161792 |
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| author | HAN Shuang, CHEN Yu, ZHANG Wei-li |
| author_facet | HAN Shuang, CHEN Yu, ZHANG Wei-li |
| author_sort | HAN Shuang, CHEN Yu, ZHANG Wei-li |
| collection | DOAJ |
| description | Objective To study the role of miR-216a in regulating the characteristics of macrophages derived from peripheral blood mononuclear cells (PBMCs). Methods Human PBMCs were isolated and cultured. The polarization model of M1 and M2 macrophages were established in vitro, and flow cytometry and real-time quantitative PCR were used to detect the cell surface markers of macrophage. Furthermore, miR-216a mimics were transfected into M2 macrophages, and the surface markers of M2 macrophage and inflammatory factors were examined. Results After overexpression of miR-216a, the expression of surface marker CD163 of M2 macrophage was significantly increased, the expressions of inflammatory cytokines TNFα and IL-1β were decreased, and the expressions of anti-inflammatory cytokines TGF-β and IL-10 were increased(P＜0.05). Conclusions miR-216a could promote the polarization of human PBMCs-derived macrophages into M2 phenotype and inhibit the expressions of pro-inflammatory cytokines. |
| first_indexed | 2024-03-08T16:55:47Z |
| format | Article |
| id | doaj.art-5612eac3baca4c7780160bf91ae5813c |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T16:55:47Z |
| publishDate | 2022-03-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-5612eac3baca4c7780160bf91ae5813c2024-01-05T02:34:23ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252022-03-0142338438810.16352/j.issn.1001-6325.2022.03.034Effect of miR-216a on macrophages derived from human peripheral blood mononuclear cellsHAN Shuang, CHEN Yu, ZHANG Wei-li0State Key Laboratory of Cardiovascular Diseases,National Center for Cardiovascular Diseases and Fuwai Hospital, CAMS & PUMC, Beijing 100037,ChinaObjective To study the role of miR-216a in regulating the characteristics of macrophages derived from peripheral blood mononuclear cells (PBMCs). Methods Human PBMCs were isolated and cultured. The polarization model of M1 and M2 macrophages were established in vitro, and flow cytometry and real-time quantitative PCR were used to detect the cell surface markers of macrophage. Furthermore, miR-216a mimics were transfected into M2 macrophages, and the surface markers of M2 macrophage and inflammatory factors were examined. Results After overexpression of miR-216a, the expression of surface marker CD163 of M2 macrophage was significantly increased, the expressions of inflammatory cytokines TNFα and IL-1β were decreased, and the expressions of anti-inflammatory cytokines TGF-β and IL-10 were increased(P＜0.05). Conclusions miR-216a could promote the polarization of human PBMCs-derived macrophages into M2 phenotype and inhibit the expressions of pro-inflammatory cytokines.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2022-42-3-384.pdfmir-216a|peripheral blood mononuclear cell|macrophage polarization|m1 macrophage|m2 macrophage |
| spellingShingle | HAN Shuang, CHEN Yu, ZHANG Wei-li Effect of miR-216a on macrophages derived from human peripheral blood mononuclear cells Jichu yixue yu linchuang mir-216a|peripheral blood mononuclear cell|macrophage polarization|m1 macrophage|m2 macrophage |
| title | Effect of miR-216a on macrophages derived from human peripheral blood mononuclear cells |
| title_full | Effect of miR-216a on macrophages derived from human peripheral blood mononuclear cells |
| title_fullStr | Effect of miR-216a on macrophages derived from human peripheral blood mononuclear cells |
| title_full_unstemmed | Effect of miR-216a on macrophages derived from human peripheral blood mononuclear cells |
| title_short | Effect of miR-216a on macrophages derived from human peripheral blood mononuclear cells |
| title_sort | effect of mir 216a on macrophages derived from human peripheral blood mononuclear cells |
| topic | mir-216a|peripheral blood mononuclear cell|macrophage polarization|m1 macrophage|m2 macrophage |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2022-42-3-384.pdf |
| work_keys_str_mv | AT hanshuangchenyuzhangweili effectofmir216aonmacrophagesderivedfromhumanperipheralbloodmononuclearcells |