Characterization of a Bacterial Symbiont Asaia sp. in the White-Backed Planthopper, Sogatella furcifera, and Its Effects on Host Fitness
The white-backed planthopper (WBPH), Sogatella furcifera Horváth (Hemiptera: Delphacidae), is an economically significant rice insect pest that harbors a primary fungal yeast-like symbiont (YLS), and some secondary bacterial symbionts like Wolbachia and Cardinium. In the present study, an additional...
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Frontiers Media S.A.
2019-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.02179/full |
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| author | Fei Li Fei Li Hongxia Hua Asad Ali Asad Ali Maolin Hou Maolin Hou Maolin Hou |
| author_facet | Fei Li Fei Li Hongxia Hua Asad Ali Asad Ali Maolin Hou Maolin Hou Maolin Hou |
| author_sort | Fei Li |
| collection | DOAJ |
| description | The white-backed planthopper (WBPH), Sogatella furcifera Horváth (Hemiptera: Delphacidae), is an economically significant rice insect pest that harbors a primary fungal yeast-like symbiont (YLS), and some secondary bacterial symbionts like Wolbachia and Cardinium. In the present study, an additional bacterial symbiont in WBPH was characterized. Phylogenetic analysis employing the 16S rRNA gene showed a bacterium closely related to Asaia of Nilaparvata lugens and Nysius expressus, and Asaia krungthepensis. TEM observation of the bacterium showed the typical morphology of Asaia sp. with signature filamentous structures in the nucleoid region. These results indicate that the bacterium belongs to Asaia. The Asaia bacterium was detected in all the tested individual adults and tissues of the laboratory WBPH population but showed varying infection rates (ca 45%) in the field collected WBPH populations. Quantitative PCR analysis revealed that Asaia sp. were significantly more abundant in WBPH females than males, and mainly distributed in the guts, fatty bodies, and salivary glands. Asaia-infected WBPH were of shorter nymphal duration and heavier adult weight than Asaia-free WBPH, while Asaia-free WBPH comparatively fed more, indicating that Asaia plays a role in improving WBPH fitness through involvement in host’s nutrient supply. |
| first_indexed | 2024-12-11T14:39:26Z |
| format | Article |
| id | doaj.art-56155b6d70f14788bf87393abb2d4c1d |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-11T14:39:26Z |
| publishDate | 2019-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-56155b6d70f14788bf87393abb2d4c1d2022-12-22T01:02:00ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-09-011010.3389/fmicb.2019.02179477742Characterization of a Bacterial Symbiont Asaia sp. in the White-Backed Planthopper, Sogatella furcifera, and Its Effects on Host FitnessFei Li0Fei Li1Hongxia Hua2Asad Ali3Asad Ali4Maolin Hou5Maolin Hou6Maolin Hou7State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, ChinaCollege of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaCollege of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, ChinaDepartment of Agriculture, Abdul Wali Khan University Mardan, Mardan, PakistanState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, ChinaScientific Observing and Experimental Station of Crop Pests in Guilin, Ministry of Agriculture, Guilin, ChinaSouthern Regional Collaborative Innovation Center for Grain and Oil Crops in China, Changsha, ChinaThe white-backed planthopper (WBPH), Sogatella furcifera Horváth (Hemiptera: Delphacidae), is an economically significant rice insect pest that harbors a primary fungal yeast-like symbiont (YLS), and some secondary bacterial symbionts like Wolbachia and Cardinium. In the present study, an additional bacterial symbiont in WBPH was characterized. Phylogenetic analysis employing the 16S rRNA gene showed a bacterium closely related to Asaia of Nilaparvata lugens and Nysius expressus, and Asaia krungthepensis. TEM observation of the bacterium showed the typical morphology of Asaia sp. with signature filamentous structures in the nucleoid region. These results indicate that the bacterium belongs to Asaia. The Asaia bacterium was detected in all the tested individual adults and tissues of the laboratory WBPH population but showed varying infection rates (ca 45%) in the field collected WBPH populations. Quantitative PCR analysis revealed that Asaia sp. were significantly more abundant in WBPH females than males, and mainly distributed in the guts, fatty bodies, and salivary glands. Asaia-infected WBPH were of shorter nymphal duration and heavier adult weight than Asaia-free WBPH, while Asaia-free WBPH comparatively fed more, indicating that Asaia plays a role in improving WBPH fitness through involvement in host’s nutrient supply.https://www.frontiersin.org/article/10.3389/fmicb.2019.02179/fullSogatella furciferabacterial symbiontAsaiainfectionhost fitness |
| spellingShingle | Fei Li Fei Li Hongxia Hua Asad Ali Asad Ali Maolin Hou Maolin Hou Maolin Hou Characterization of a Bacterial Symbiont Asaia sp. in the White-Backed Planthopper, Sogatella furcifera, and Its Effects on Host Fitness Frontiers in Microbiology Sogatella furcifera bacterial symbiont Asaia infection host fitness |
| title | Characterization of a Bacterial Symbiont Asaia sp. in the White-Backed Planthopper, Sogatella furcifera, and Its Effects on Host Fitness |
| title_full | Characterization of a Bacterial Symbiont Asaia sp. in the White-Backed Planthopper, Sogatella furcifera, and Its Effects on Host Fitness |
| title_fullStr | Characterization of a Bacterial Symbiont Asaia sp. in the White-Backed Planthopper, Sogatella furcifera, and Its Effects on Host Fitness |
| title_full_unstemmed | Characterization of a Bacterial Symbiont Asaia sp. in the White-Backed Planthopper, Sogatella furcifera, and Its Effects on Host Fitness |
| title_short | Characterization of a Bacterial Symbiont Asaia sp. in the White-Backed Planthopper, Sogatella furcifera, and Its Effects on Host Fitness |
| title_sort | characterization of a bacterial symbiont asaia sp in the white backed planthopper sogatella furcifera and its effects on host fitness |
| topic | Sogatella furcifera bacterial symbiont Asaia infection host fitness |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.02179/full |
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