Ocular and uteroplacental pathology in a macaque pregnancy with congenital Zika virus infection.
Congenital Zika virus (ZIKV) infection impacts fetal development and pregnancy outcomes. We infected a pregnant rhesus macaque with a Puerto Rican ZIKV isolate in the first trimester. The pregnancy was complicated by preterm premature rupture of membranes (PPROM), intraamniotic bacterial infection a...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2018-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5790226?pdf=render |
| _version_ | 1818985745733386240 |
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| author | Emma L Mohr Lindsey N Block Christina M Newman Laurel M Stewart Michelle Koenig Matthew Semler Meghan E Breitbach Leandro B C Teixeira Xiankun Zeng Andrea M Weiler Gabrielle L Barry Troy H Thoong Gregory J Wiepz Dawn M Dudley Heather A Simmons Andres Mejia Terry K Morgan M Shahriar Salamat Sarah Kohn Kathleen M Antony Matthew T Aliota Mariel S Mohns Jennifer M Hayes Nancy Schultz-Darken Michele L Schotzko Eric Peterson Saverio Capuano Jorge E Osorio Shelby L O'Connor Thomas C Friedrich David H O'Connor Thaddeus G Golos |
| author_facet | Emma L Mohr Lindsey N Block Christina M Newman Laurel M Stewart Michelle Koenig Matthew Semler Meghan E Breitbach Leandro B C Teixeira Xiankun Zeng Andrea M Weiler Gabrielle L Barry Troy H Thoong Gregory J Wiepz Dawn M Dudley Heather A Simmons Andres Mejia Terry K Morgan M Shahriar Salamat Sarah Kohn Kathleen M Antony Matthew T Aliota Mariel S Mohns Jennifer M Hayes Nancy Schultz-Darken Michele L Schotzko Eric Peterson Saverio Capuano Jorge E Osorio Shelby L O'Connor Thomas C Friedrich David H O'Connor Thaddeus G Golos |
| author_sort | Emma L Mohr |
| collection | DOAJ |
| description | Congenital Zika virus (ZIKV) infection impacts fetal development and pregnancy outcomes. We infected a pregnant rhesus macaque with a Puerto Rican ZIKV isolate in the first trimester. The pregnancy was complicated by preterm premature rupture of membranes (PPROM), intraamniotic bacterial infection and fetal demise 49 days post infection (gestational day 95). Significant pathology at the maternal-fetal interface included acute chorioamnionitis, placental infarcts, and leukocytoclastic vasculitis of the myometrial radial arteries. ZIKV RNA was disseminated throughout fetal tissues and maternal immune system tissues at necropsy, as assessed by quantitative RT-PCR for viral RNA. Replicating ZIKV was identified in fetal tissues, maternal uterus, and maternal spleen by fluorescent in situ hybridization for viral replication intermediates. Fetal ocular pathology included a choroidal coloboma, suspected anterior segment dysgenesis, and a dysplastic retina. This is the first report of ocular pathology and prolonged viral replication in both maternal and fetal tissues following congenital ZIKV infection in a rhesus macaque. PPROM followed by fetal demise and severe pathology of the visual system have not been described in macaque congenital ZIKV infection previously. While this case of ZIKV infection during pregnancy was complicated by bacterial infection with PPROM, the role of ZIKV on this outcome cannot be precisely defined, and further nonhuman primate studies will determine if increased risk for PPROM or other adverse pregnancy outcomes are associated with congenital ZIKV infection. |
| first_indexed | 2024-12-20T18:39:47Z |
| format | Article |
| id | doaj.art-561e5af15c784f1080251be4b6e200fd |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T18:39:47Z |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-561e5af15c784f1080251be4b6e200fd2022-12-21T19:29:51ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01131e019061710.1371/journal.pone.0190617Ocular and uteroplacental pathology in a macaque pregnancy with congenital Zika virus infection.Emma L MohrLindsey N BlockChristina M NewmanLaurel M StewartMichelle KoenigMatthew SemlerMeghan E BreitbachLeandro B C TeixeiraXiankun ZengAndrea M WeilerGabrielle L BarryTroy H ThoongGregory J WiepzDawn M DudleyHeather A SimmonsAndres MejiaTerry K MorganM Shahriar SalamatSarah KohnKathleen M AntonyMatthew T AliotaMariel S MohnsJennifer M HayesNancy Schultz-DarkenMichele L SchotzkoEric PetersonSaverio CapuanoJorge E OsorioShelby L O'ConnorThomas C FriedrichDavid H O'ConnorThaddeus G GolosCongenital Zika virus (ZIKV) infection impacts fetal development and pregnancy outcomes. We infected a pregnant rhesus macaque with a Puerto Rican ZIKV isolate in the first trimester. The pregnancy was complicated by preterm premature rupture of membranes (PPROM), intraamniotic bacterial infection and fetal demise 49 days post infection (gestational day 95). Significant pathology at the maternal-fetal interface included acute chorioamnionitis, placental infarcts, and leukocytoclastic vasculitis of the myometrial radial arteries. ZIKV RNA was disseminated throughout fetal tissues and maternal immune system tissues at necropsy, as assessed by quantitative RT-PCR for viral RNA. Replicating ZIKV was identified in fetal tissues, maternal uterus, and maternal spleen by fluorescent in situ hybridization for viral replication intermediates. Fetal ocular pathology included a choroidal coloboma, suspected anterior segment dysgenesis, and a dysplastic retina. This is the first report of ocular pathology and prolonged viral replication in both maternal and fetal tissues following congenital ZIKV infection in a rhesus macaque. PPROM followed by fetal demise and severe pathology of the visual system have not been described in macaque congenital ZIKV infection previously. While this case of ZIKV infection during pregnancy was complicated by bacterial infection with PPROM, the role of ZIKV on this outcome cannot be precisely defined, and further nonhuman primate studies will determine if increased risk for PPROM or other adverse pregnancy outcomes are associated with congenital ZIKV infection.http://europepmc.org/articles/PMC5790226?pdf=render |
| spellingShingle | Emma L Mohr Lindsey N Block Christina M Newman Laurel M Stewart Michelle Koenig Matthew Semler Meghan E Breitbach Leandro B C Teixeira Xiankun Zeng Andrea M Weiler Gabrielle L Barry Troy H Thoong Gregory J Wiepz Dawn M Dudley Heather A Simmons Andres Mejia Terry K Morgan M Shahriar Salamat Sarah Kohn Kathleen M Antony Matthew T Aliota Mariel S Mohns Jennifer M Hayes Nancy Schultz-Darken Michele L Schotzko Eric Peterson Saverio Capuano Jorge E Osorio Shelby L O'Connor Thomas C Friedrich David H O'Connor Thaddeus G Golos Ocular and uteroplacental pathology in a macaque pregnancy with congenital Zika virus infection. PLoS ONE |
| title | Ocular and uteroplacental pathology in a macaque pregnancy with congenital Zika virus infection. |
| title_full | Ocular and uteroplacental pathology in a macaque pregnancy with congenital Zika virus infection. |
| title_fullStr | Ocular and uteroplacental pathology in a macaque pregnancy with congenital Zika virus infection. |
| title_full_unstemmed | Ocular and uteroplacental pathology in a macaque pregnancy with congenital Zika virus infection. |
| title_short | Ocular and uteroplacental pathology in a macaque pregnancy with congenital Zika virus infection. |
| title_sort | ocular and uteroplacental pathology in a macaque pregnancy with congenital zika virus infection |
| url | http://europepmc.org/articles/PMC5790226?pdf=render |
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