Comparative investigation on heterotrophic denitrification driven by different biodegradable polymers for nitrate removal in mariculture wastewater: Organic carbon release, denitrification performance, and microbial community
Heterotrophic denitrification is widely studied to purify freshwater wastewater, but its application to seawater wastewater is rarely reported. In this study, two types of agricultural wastes and two types of synthetic polymers were selected as solid carbon sources in denitrification process to expl...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1141362/full |
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| author | Yuna Feng Yuna Feng Lu Wang Zhendong Yin Zhendong Yin Zhengguo Cui Zhengguo Cui Keming Qu Keming Qu Dawei Wang Dawei Wang Zhanying Wang Zhanying Wang Shengmin Zhu Shengmin Zhu Hongwu Cui Hongwu Cui |
| author_facet | Yuna Feng Yuna Feng Lu Wang Zhendong Yin Zhendong Yin Zhengguo Cui Zhengguo Cui Keming Qu Keming Qu Dawei Wang Dawei Wang Zhanying Wang Zhanying Wang Shengmin Zhu Shengmin Zhu Hongwu Cui Hongwu Cui |
| author_sort | Yuna Feng |
| collection | DOAJ |
| description | Heterotrophic denitrification is widely studied to purify freshwater wastewater, but its application to seawater wastewater is rarely reported. In this study, two types of agricultural wastes and two types of synthetic polymers were selected as solid carbon sources in denitrification process to explore their effects on the purification capacity of low-C/N marine recirculating aquaculture wastewater (NO3−-N 30 mg/L, salinity 32‰). The surface properties of reed straw (RS), corn cob (CC), polycaprolactone (PCL) and poly3-hydroxybutyrate-hydroxypropionate (PHBV) were evaluated by Brunauer–Emmett–Teller, Scanning electron microscope and Fourier-transform infrared spectroscopy. Short-chain fatty acids, dissolved organic carbon (DOC), and chemical oxygen demand (COD) equivalents were used to analyze the carbon release capacity. Results showed that agricultural waste had higher carbon release capacity than PCL and PHBV. The cumulative DOC and COD of agricultural waste were 0.56–12.65 and 1.15–18.75 mg/g, respectively, while those for synthetic polymers were 0.07–1.473 and 0.045–1.425 mg/g, respectively. The removal efficiency of nitrate nitrogen (NO3−-N) was CC 70.80%, PCL 53.64%, RS 42.51%, and PHBV 41.35%. Microbial community analysis showed that Proteobacteria and Firmicutes were the most abundant phyla in agricultural wastes and biodegradable natural or synthetic polymers. Quantitative real-time PCR indicated the conversion from nitrate to nitrogen was achieved in all four carbon source systems, and all six genes had the highest copy number in CC. The contents of medium nitrate reductase, nitrite reductase and nitrous oxide reductase genes in agricultural wastes were higher than those in synthetic polymers. In summary, CC is an ideal carbon source for denitrification technology to purify low C/N recirculating mariculture wastewater. |
| first_indexed | 2024-04-10T09:25:20Z |
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| id | doaj.art-56201b4db4b04025b79fae4d579097a1 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-10T09:25:20Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-56201b4db4b04025b79fae4d579097a12023-02-20T05:28:13ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-02-011410.3389/fmicb.2023.11413621141362Comparative investigation on heterotrophic denitrification driven by different biodegradable polymers for nitrate removal in mariculture wastewater: Organic carbon release, denitrification performance, and microbial communityYuna Feng0Yuna Feng1Lu Wang2Zhendong Yin3Zhendong Yin4Zhengguo Cui5Zhengguo Cui6Keming Qu7Keming Qu8Dawei Wang9Dawei Wang10Zhanying Wang11Zhanying Wang12Shengmin Zhu13Shengmin Zhu14Hongwu Cui15Hongwu Cui16National Experimental Teaching Demonstration Center for Aquatic Science, National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, ChinaKey Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, ChinaMarine Life Research Center, Laoshan Laboratory, Qingdao, ChinaKey Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, ChinaCollege of Chemistry and Chemical Engineering, Ocean University of China, Qingdao, ChinaKey Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, ChinaMarine Life Research Center, Laoshan Laboratory, Qingdao, ChinaKey Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, ChinaMarine Life Research Center, Laoshan Laboratory, Qingdao, ChinaNational Experimental Teaching Demonstration Center for Aquatic Science, National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, ChinaKey Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, ChinaNational Experimental Teaching Demonstration Center for Aquatic Science, National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, ChinaKey Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, ChinaKey Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, ChinaCollege of Chemistry and Chemical Engineering, Ocean University of China, Qingdao, ChinaKey Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, ChinaMarine Life Research Center, Laoshan Laboratory, Qingdao, ChinaHeterotrophic denitrification is widely studied to purify freshwater wastewater, but its application to seawater wastewater is rarely reported. In this study, two types of agricultural wastes and two types of synthetic polymers were selected as solid carbon sources in denitrification process to explore their effects on the purification capacity of low-C/N marine recirculating aquaculture wastewater (NO3−-N 30 mg/L, salinity 32‰). The surface properties of reed straw (RS), corn cob (CC), polycaprolactone (PCL) and poly3-hydroxybutyrate-hydroxypropionate (PHBV) were evaluated by Brunauer–Emmett–Teller, Scanning electron microscope and Fourier-transform infrared spectroscopy. Short-chain fatty acids, dissolved organic carbon (DOC), and chemical oxygen demand (COD) equivalents were used to analyze the carbon release capacity. Results showed that agricultural waste had higher carbon release capacity than PCL and PHBV. The cumulative DOC and COD of agricultural waste were 0.56–12.65 and 1.15–18.75 mg/g, respectively, while those for synthetic polymers were 0.07–1.473 and 0.045–1.425 mg/g, respectively. The removal efficiency of nitrate nitrogen (NO3−-N) was CC 70.80%, PCL 53.64%, RS 42.51%, and PHBV 41.35%. Microbial community analysis showed that Proteobacteria and Firmicutes were the most abundant phyla in agricultural wastes and biodegradable natural or synthetic polymers. Quantitative real-time PCR indicated the conversion from nitrate to nitrogen was achieved in all four carbon source systems, and all six genes had the highest copy number in CC. The contents of medium nitrate reductase, nitrite reductase and nitrous oxide reductase genes in agricultural wastes were higher than those in synthetic polymers. In summary, CC is an ideal carbon source for denitrification technology to purify low C/N recirculating mariculture wastewater.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1141362/fullsolid carbon sourceslow-releasenitrate removalmariculture wastewaterlow C/N |
| spellingShingle | Yuna Feng Yuna Feng Lu Wang Zhendong Yin Zhendong Yin Zhengguo Cui Zhengguo Cui Keming Qu Keming Qu Dawei Wang Dawei Wang Zhanying Wang Zhanying Wang Shengmin Zhu Shengmin Zhu Hongwu Cui Hongwu Cui Comparative investigation on heterotrophic denitrification driven by different biodegradable polymers for nitrate removal in mariculture wastewater: Organic carbon release, denitrification performance, and microbial community Frontiers in Microbiology solid carbon source slow-release nitrate removal mariculture wastewater low C/N |
| title | Comparative investigation on heterotrophic denitrification driven by different biodegradable polymers for nitrate removal in mariculture wastewater: Organic carbon release, denitrification performance, and microbial community |
| title_full | Comparative investigation on heterotrophic denitrification driven by different biodegradable polymers for nitrate removal in mariculture wastewater: Organic carbon release, denitrification performance, and microbial community |
| title_fullStr | Comparative investigation on heterotrophic denitrification driven by different biodegradable polymers for nitrate removal in mariculture wastewater: Organic carbon release, denitrification performance, and microbial community |
| title_full_unstemmed | Comparative investigation on heterotrophic denitrification driven by different biodegradable polymers for nitrate removal in mariculture wastewater: Organic carbon release, denitrification performance, and microbial community |
| title_short | Comparative investigation on heterotrophic denitrification driven by different biodegradable polymers for nitrate removal in mariculture wastewater: Organic carbon release, denitrification performance, and microbial community |
| title_sort | comparative investigation on heterotrophic denitrification driven by different biodegradable polymers for nitrate removal in mariculture wastewater organic carbon release denitrification performance and microbial community |
| topic | solid carbon source slow-release nitrate removal mariculture wastewater low C/N |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1141362/full |
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