Analyzing cannabinoid-induced abnormal behavior in a zebrafish model.
In this study, we investigated locomotor activity and responses to repeated light and dark stimuli to assess cannabinoid-induced abnormal behavior in zebrafish larvae (Danio rerio), as an alternative to standard rodent models. To induce the desired responses, we used cannabidiol and WIN55,212-2, two...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2020-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0236606 |
| _version_ | 1819017089040515072 |
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| author | Akihiro Hasumi Hideyuki Maeda Ken-Ichi Yoshida |
| author_facet | Akihiro Hasumi Hideyuki Maeda Ken-Ichi Yoshida |
| author_sort | Akihiro Hasumi |
| collection | DOAJ |
| description | In this study, we investigated locomotor activity and responses to repeated light and dark stimuli to assess cannabinoid-induced abnormal behavior in zebrafish larvae (Danio rerio), as an alternative to standard rodent models. To induce the desired responses, we used cannabidiol and WIN55,212-2, two major cannabinoid components. A repeated light and dark test was used to assess how drug exposure influences locomotory responses. Larvae were examined after moderate cannabidiol and WIN55,212-2 exposure and at 24 h after transfer to untreated water. We found that cannabidiol did not produce a dose-dependent inhibitory effect on locomotor activity, with both 0.5 and 10 μg/mL concentrations reducing movement velocity and the total distance moved. However, 10 μg/mL cannabidiol was observed to attenuate the responses of larvae exposed to darkness. No differences were detected between the control and cannabidiol-treated groups after 24 h in fresh water. Fish treated with WIN55,212-2 at 0.5 and 1 μg/mL showed virtually no activity, even in darkness, whereas a concentration of 10 μg/mL induced mortality. A 24-h period in fresh water had the effect of reversing most of the drug-induced immobilization, even in the WIN55,212-2-treated groups. Larvae were also evaluated for their responses to cannabidiol subsequent to an initial exposure to WIN55,212-2, and it was accordingly found that treatment with cannabidiol could attenuate WIN55,212-2-induced abnormal immobilization, whereas equivalent doses of cannabidiol and WIN55,212-2 produced a mixed response. In conclusion, the behavioral effects of the two cannabinoids cannabidiol and WIN55,212-2 appear to be ratio dependent. Furthermore, the repeated light and dark test could serve as a suitable method for assaying drug-induced behavior. |
| first_indexed | 2024-12-21T02:57:58Z |
| format | Article |
| id | doaj.art-5620248174cc40f18fee4d016436b00c |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T02:57:58Z |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-5620248174cc40f18fee4d016436b00c2022-12-21T19:18:16ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-011510e023660610.1371/journal.pone.0236606Analyzing cannabinoid-induced abnormal behavior in a zebrafish model.Akihiro HasumiHideyuki MaedaKen-Ichi YoshidaIn this study, we investigated locomotor activity and responses to repeated light and dark stimuli to assess cannabinoid-induced abnormal behavior in zebrafish larvae (Danio rerio), as an alternative to standard rodent models. To induce the desired responses, we used cannabidiol and WIN55,212-2, two major cannabinoid components. A repeated light and dark test was used to assess how drug exposure influences locomotory responses. Larvae were examined after moderate cannabidiol and WIN55,212-2 exposure and at 24 h after transfer to untreated water. We found that cannabidiol did not produce a dose-dependent inhibitory effect on locomotor activity, with both 0.5 and 10 μg/mL concentrations reducing movement velocity and the total distance moved. However, 10 μg/mL cannabidiol was observed to attenuate the responses of larvae exposed to darkness. No differences were detected between the control and cannabidiol-treated groups after 24 h in fresh water. Fish treated with WIN55,212-2 at 0.5 and 1 μg/mL showed virtually no activity, even in darkness, whereas a concentration of 10 μg/mL induced mortality. A 24-h period in fresh water had the effect of reversing most of the drug-induced immobilization, even in the WIN55,212-2-treated groups. Larvae were also evaluated for their responses to cannabidiol subsequent to an initial exposure to WIN55,212-2, and it was accordingly found that treatment with cannabidiol could attenuate WIN55,212-2-induced abnormal immobilization, whereas equivalent doses of cannabidiol and WIN55,212-2 produced a mixed response. In conclusion, the behavioral effects of the two cannabinoids cannabidiol and WIN55,212-2 appear to be ratio dependent. Furthermore, the repeated light and dark test could serve as a suitable method for assaying drug-induced behavior.https://doi.org/10.1371/journal.pone.0236606 |
| spellingShingle | Akihiro Hasumi Hideyuki Maeda Ken-Ichi Yoshida Analyzing cannabinoid-induced abnormal behavior in a zebrafish model. PLoS ONE |
| title | Analyzing cannabinoid-induced abnormal behavior in a zebrafish model. |
| title_full | Analyzing cannabinoid-induced abnormal behavior in a zebrafish model. |
| title_fullStr | Analyzing cannabinoid-induced abnormal behavior in a zebrafish model. |
| title_full_unstemmed | Analyzing cannabinoid-induced abnormal behavior in a zebrafish model. |
| title_short | Analyzing cannabinoid-induced abnormal behavior in a zebrafish model. |
| title_sort | analyzing cannabinoid induced abnormal behavior in a zebrafish model |
| url | https://doi.org/10.1371/journal.pone.0236606 |
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