Biological Control Effect of Antagonistic Bacteria on Potato Black Scurf Disease Caused by <i>Rhizoctonia solani</i>
Potato black scurf, caused by <i>Rhizoctonia solani</i>, is a severe soil-borne disease that affects the quality and production of potatoes worldwide. In our study, we used <i>Paenibacillus polymyxa</i> YF and <i>Bacillus amyloliquefaciens</i> HT to determine the...
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MDPI AG
2024-02-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/14/2/351 |
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| author | Qingqing Lan Yang Liu Rongrong Mu Xuetao Wang Qian Zhou Rehmat Islam Xu Su Yongqiang Tian |
| author_facet | Qingqing Lan Yang Liu Rongrong Mu Xuetao Wang Qian Zhou Rehmat Islam Xu Su Yongqiang Tian |
| author_sort | Qingqing Lan |
| collection | DOAJ |
| description | Potato black scurf, caused by <i>Rhizoctonia solani</i>, is a severe soil-borne disease that affects the quality and production of potatoes worldwide. In our study, we used <i>Paenibacillus polymyxa</i> YF and <i>Bacillus amyloliquefaciens</i> HT to determine the antagonistic ability of <i>R. solani</i>, with a particular focus on the antagonistic action of <i>P. polymyxa</i> YF to <i>R. solani</i> and its biocontrol effect on potato black scurf. In fermentation, filtrate assay, 50% filtrate of <i>P. polymyxa</i> YF and <i>B. amyloliquefaciens</i> HT inhibited the growth of <i>R. solani</i> by 85.55% and 82.86%, respectively. Microscopic observations showed notable morphological changes with mycelial collapse, atrophy, and deformation following treatment with the antagonistic filtrates. Moreover, cell membrane permeability results showed increased conductivity in bacteria-treated samples compared to the control. <i>P. polymyxa</i> YF exhibited stable colonization on potato plants and secreted various extracellular enzymes (protease, amylase, and cellulase), along with the synthetic substances with growth-promoting effects, such as siderophores and Indole-3-acetic acid (IAA). Whether it is in the excised tissue inoculation or potted experiment, the negative control showed the highest rank of disease symptoms. In the pot experiment, after YF treatment, physiological parameters showed remarkable changes in plant height, root length, stem thickness, and dry and fresh weight. Compared to blank control, the activities of the four resistant enzymes increased significantly in the <i>P. polymyxa</i> YF treatment group. The upregulation in the <i>P. polymyxa</i> YF group was 4.04, 0.54, 0.46, and 3.10 times, respectively. PCR analysis identified genes in both bacterial strains coding for antimicrobial lipopeptides, including fenB, ituC, and srfAA, which are associated with fengycin, iturin, and surfactin synthesis. These findings demonstrated that <i>P. polymyxa</i> YF has a prominent antagonistic effect on <i>R. solani</i>, suggesting its potential as an effective biological control agent for controlling potato black scurf. |
| first_indexed | 2024-03-07T22:45:27Z |
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| id | doaj.art-56226b62b3944bebbda99e7bf53e7b7b |
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| last_indexed | 2024-03-07T22:45:27Z |
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| spelling | doaj.art-56226b62b3944bebbda99e7bf53e7b7b2024-02-23T15:04:16ZengMDPI AGAgronomy2073-43952024-02-0114235110.3390/agronomy14020351Biological Control Effect of Antagonistic Bacteria on Potato Black Scurf Disease Caused by <i>Rhizoctonia solani</i>Qingqing Lan0Yang Liu1Rongrong Mu2Xuetao Wang3Qian Zhou4Rehmat Islam5Xu Su6Yongqiang Tian7Laboratory of Tian Yongqiang Scientific Research 101, College of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou 730070, ChinaLaboratory of Tian Yongqiang Scientific Research 101, College of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou 730070, ChinaLaboratory of Tian Yongqiang Scientific Research 101, College of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou 730070, ChinaLaboratory of Tian Yongqiang Scientific Research 101, College of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou 730070, ChinaLaboratory of Tian Yongqiang Scientific Research 101, College of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou 730070, ChinaKey Laboratory of Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi’an 710072, ChinaKey Laboratory of Biodiversity Formation Mechanism and Comprehensive Utilization of the Qinghai-Tibet Plateau in Qinghai Province, Qinghai Normal University, Xining 810008, ChinaLaboratory of Tian Yongqiang Scientific Research 101, College of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou 730070, ChinaPotato black scurf, caused by <i>Rhizoctonia solani</i>, is a severe soil-borne disease that affects the quality and production of potatoes worldwide. In our study, we used <i>Paenibacillus polymyxa</i> YF and <i>Bacillus amyloliquefaciens</i> HT to determine the antagonistic ability of <i>R. solani</i>, with a particular focus on the antagonistic action of <i>P. polymyxa</i> YF to <i>R. solani</i> and its biocontrol effect on potato black scurf. In fermentation, filtrate assay, 50% filtrate of <i>P. polymyxa</i> YF and <i>B. amyloliquefaciens</i> HT inhibited the growth of <i>R. solani</i> by 85.55% and 82.86%, respectively. Microscopic observations showed notable morphological changes with mycelial collapse, atrophy, and deformation following treatment with the antagonistic filtrates. Moreover, cell membrane permeability results showed increased conductivity in bacteria-treated samples compared to the control. <i>P. polymyxa</i> YF exhibited stable colonization on potato plants and secreted various extracellular enzymes (protease, amylase, and cellulase), along with the synthetic substances with growth-promoting effects, such as siderophores and Indole-3-acetic acid (IAA). Whether it is in the excised tissue inoculation or potted experiment, the negative control showed the highest rank of disease symptoms. In the pot experiment, after YF treatment, physiological parameters showed remarkable changes in plant height, root length, stem thickness, and dry and fresh weight. Compared to blank control, the activities of the four resistant enzymes increased significantly in the <i>P. polymyxa</i> YF treatment group. The upregulation in the <i>P. polymyxa</i> YF group was 4.04, 0.54, 0.46, and 3.10 times, respectively. PCR analysis identified genes in both bacterial strains coding for antimicrobial lipopeptides, including fenB, ituC, and srfAA, which are associated with fengycin, iturin, and surfactin synthesis. These findings demonstrated that <i>P. polymyxa</i> YF has a prominent antagonistic effect on <i>R. solani</i>, suggesting its potential as an effective biological control agent for controlling potato black scurf.https://www.mdpi.com/2073-4395/14/2/351antagonistic effectbiocontrol bacteriagrowth promotionpotato black scurf<i>Rhizoctonia solani</i> |
| spellingShingle | Qingqing Lan Yang Liu Rongrong Mu Xuetao Wang Qian Zhou Rehmat Islam Xu Su Yongqiang Tian Biological Control Effect of Antagonistic Bacteria on Potato Black Scurf Disease Caused by <i>Rhizoctonia solani</i> Agronomy antagonistic effect biocontrol bacteria growth promotion potato black scurf <i>Rhizoctonia solani</i> |
| title | Biological Control Effect of Antagonistic Bacteria on Potato Black Scurf Disease Caused by <i>Rhizoctonia solani</i> |
| title_full | Biological Control Effect of Antagonistic Bacteria on Potato Black Scurf Disease Caused by <i>Rhizoctonia solani</i> |
| title_fullStr | Biological Control Effect of Antagonistic Bacteria on Potato Black Scurf Disease Caused by <i>Rhizoctonia solani</i> |
| title_full_unstemmed | Biological Control Effect of Antagonistic Bacteria on Potato Black Scurf Disease Caused by <i>Rhizoctonia solani</i> |
| title_short | Biological Control Effect of Antagonistic Bacteria on Potato Black Scurf Disease Caused by <i>Rhizoctonia solani</i> |
| title_sort | biological control effect of antagonistic bacteria on potato black scurf disease caused by i rhizoctonia solani i |
| topic | antagonistic effect biocontrol bacteria growth promotion potato black scurf <i>Rhizoctonia solani</i> |
| url | https://www.mdpi.com/2073-4395/14/2/351 |
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