Microflow cytometer chip with multiple electro-rotation units for measuring electrical properties of single yeast cells
At present, many single-cell electrical parameter measurement techniques have exhibit broad application prospects, but there are still some limitations such as complex single-cell operation and time-consuming measurement. This paper proposed a microflow cytometer chip with multiple electro-rotation...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-05-01
|
| Series: | Biosensors and Bioelectronics: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S259013702300016X |
| _version_ | 1827987852537888768 |
|---|---|
| author | Liang Huang Haisheng Ye Haojie Xia Yizhong Shen |
| author_facet | Liang Huang Haisheng Ye Haojie Xia Yizhong Shen |
| author_sort | Liang Huang |
| collection | DOAJ |
| description | At present, many single-cell electrical parameter measurement techniques have exhibit broad application prospects, but there are still some limitations such as complex single-cell operation and time-consuming measurement. This paper proposed a microflow cytometer chip with multiple electro-rotation units for measuring the electrical properties of single cells. The microdevice leverages three serial electro-rotation units to rapidly obtain rotation speeds under different signal frequencies. According to the rotation results, the electrical parameters (membrane permittivity and cytoplasmic conductivity) are estimated by using Maxwell's mixture equation fitting and neural network fitting methods respectively. We demonstrated the measurement advantages of combining microflow and electro-rotation techniques. Experimental results show that the cytometer measurement method greatly improves the throughput of single-cell characterization with a guaranteed error of less than 0.8%. The microdevice provides a microfluidic platform capable of characterizing the electrical properties of cells and the possibility of combining with neural networks. |
| first_indexed | 2024-04-09T23:53:26Z |
| format | Article |
| id | doaj.art-562362145b6748e29fb8ef10769e5940 |
| institution | Directory Open Access Journal |
| issn | 2590-1370 |
| language | English |
| last_indexed | 2024-04-09T23:53:26Z |
| publishDate | 2023-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biosensors and Bioelectronics: X |
| spelling | doaj.art-562362145b6748e29fb8ef10769e59402023-03-17T04:33:56ZengElsevierBiosensors and Bioelectronics: X2590-13702023-05-0113100319Microflow cytometer chip with multiple electro-rotation units for measuring electrical properties of single yeast cellsLiang Huang0Haisheng Ye1Haojie Xia2Yizhong Shen3Anhui Province Key Laboratory of Measuring Theory and Precision Instrument, School of Instrument Science and Optoelectronics Engineering, Hefei University of Technology, Hefei, 230009, ChinaAnhui Province Key Laboratory of Measuring Theory and Precision Instrument, School of Instrument Science and Optoelectronics Engineering, Hefei University of Technology, Hefei, 230009, ChinaAnhui Province Key Laboratory of Measuring Theory and Precision Instrument, School of Instrument Science and Optoelectronics Engineering, Hefei University of Technology, Hefei, 230009, China; Corresponding author.School of Food and Biological Engineering, Engineering Research Center of Bio-Process, Ministry of Education, Hefei University of Technology, Hefei, 230009, China; Corresponding author.At present, many single-cell electrical parameter measurement techniques have exhibit broad application prospects, but there are still some limitations such as complex single-cell operation and time-consuming measurement. This paper proposed a microflow cytometer chip with multiple electro-rotation units for measuring the electrical properties of single cells. The microdevice leverages three serial electro-rotation units to rapidly obtain rotation speeds under different signal frequencies. According to the rotation results, the electrical parameters (membrane permittivity and cytoplasmic conductivity) are estimated by using Maxwell's mixture equation fitting and neural network fitting methods respectively. We demonstrated the measurement advantages of combining microflow and electro-rotation techniques. Experimental results show that the cytometer measurement method greatly improves the throughput of single-cell characterization with a guaranteed error of less than 0.8%. The microdevice provides a microfluidic platform capable of characterizing the electrical properties of cells and the possibility of combining with neural networks.http://www.sciencedirect.com/science/article/pii/S259013702300016XMicrofluidicsDielectrophoresisElectro-rotationElectrical propertiesNeural network |
| spellingShingle | Liang Huang Haisheng Ye Haojie Xia Yizhong Shen Microflow cytometer chip with multiple electro-rotation units for measuring electrical properties of single yeast cells Biosensors and Bioelectronics: X Microfluidics Dielectrophoresis Electro-rotation Electrical properties Neural network |
| title | Microflow cytometer chip with multiple electro-rotation units for measuring electrical properties of single yeast cells |
| title_full | Microflow cytometer chip with multiple electro-rotation units for measuring electrical properties of single yeast cells |
| title_fullStr | Microflow cytometer chip with multiple electro-rotation units for measuring electrical properties of single yeast cells |
| title_full_unstemmed | Microflow cytometer chip with multiple electro-rotation units for measuring electrical properties of single yeast cells |
| title_short | Microflow cytometer chip with multiple electro-rotation units for measuring electrical properties of single yeast cells |
| title_sort | microflow cytometer chip with multiple electro rotation units for measuring electrical properties of single yeast cells |
| topic | Microfluidics Dielectrophoresis Electro-rotation Electrical properties Neural network |
| url | http://www.sciencedirect.com/science/article/pii/S259013702300016X |
| work_keys_str_mv | AT lianghuang microflowcytometerchipwithmultipleelectrorotationunitsformeasuringelectricalpropertiesofsingleyeastcells AT haishengye microflowcytometerchipwithmultipleelectrorotationunitsformeasuringelectricalpropertiesofsingleyeastcells AT haojiexia microflowcytometerchipwithmultipleelectrorotationunitsformeasuringelectricalpropertiesofsingleyeastcells AT yizhongshen microflowcytometerchipwithmultipleelectrorotationunitsformeasuringelectricalpropertiesofsingleyeastcells |