Rapid Enzyme-Linked Immunosorbent Assay for the Detection of Hantavirus-Specific Antibodies in Divergent Small Mammals
We assessed the utility of an enzyme-linked immunosorbent assay (ELISA) for the detection of hantavirus-specific antibodies from sera of Oligoryzomys longicaudatus, the principal reservoir of Andes virus (ANDV), using an antigen previously developed for detection of antibodies to Sin Nombre virus (S...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2014-05-01
|
| Series: | Viruses |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1999-4915/6/5/2028 |
| _version_ | 1828352987886518272 |
|---|---|
| author | Karla Cautivo Tony Schountz Mariana Acuña-Retamar Marcela Ferrés Fernando Torres-Pérez |
| author_facet | Karla Cautivo Tony Schountz Mariana Acuña-Retamar Marcela Ferrés Fernando Torres-Pérez |
| author_sort | Karla Cautivo |
| collection | DOAJ |
| description | We assessed the utility of an enzyme-linked immunosorbent assay (ELISA) for the detection of hantavirus-specific antibodies from sera of Oligoryzomys longicaudatus, the principal reservoir of Andes virus (ANDV), using an antigen previously developed for detection of antibodies to Sin Nombre virus (SNV) in sera from Peromyscus maniculatus. The assay uses a protein A/G horseradish peroxidase conjugate and can be performed in as little as 1.5 hours. Serum samples from Oligoryzomys longicaudatus collected in central-south Chile were used and the assay identified several that were antibody positive. This assay can be used for the rapid detection of antibodies to divergent hantaviruses from geographically and phylogenetically distant rodent species. |
| first_indexed | 2024-04-14T02:01:22Z |
| format | Article |
| id | doaj.art-5626c6d098ed4b35913f968028c7c8ed |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-04-14T02:01:22Z |
| publishDate | 2014-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-5626c6d098ed4b35913f968028c7c8ed2022-12-22T02:18:50ZengMDPI AGViruses1999-49152014-05-01652028203710.3390/v6052028v6052028Rapid Enzyme-Linked Immunosorbent Assay for the Detection of Hantavirus-Specific Antibodies in Divergent Small MammalsKarla Cautivo0Tony Schountz1Mariana Acuña-Retamar2Marcela Ferrés3Fernando Torres-Pérez4Instituto de Biología, Pontificia Universidad Católica de Valparaíso, Valparaíso 2373223, ChileDepartment of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USAFacultad de Cs Veterinarias y Pecuarias, Universidad de Chile, Santiago 8820808, ChileLaboratorio de Infectología y Virología Molecular, Escuela de Medicina, Pontificia Universidad Católica de Chile, Santiago 8330024, ChileInstituto de Biología, Pontificia Universidad Católica de Valparaíso, Valparaíso 2373223, ChileWe assessed the utility of an enzyme-linked immunosorbent assay (ELISA) for the detection of hantavirus-specific antibodies from sera of Oligoryzomys longicaudatus, the principal reservoir of Andes virus (ANDV), using an antigen previously developed for detection of antibodies to Sin Nombre virus (SNV) in sera from Peromyscus maniculatus. The assay uses a protein A/G horseradish peroxidase conjugate and can be performed in as little as 1.5 hours. Serum samples from Oligoryzomys longicaudatus collected in central-south Chile were used and the assay identified several that were antibody positive. This assay can be used for the rapid detection of antibodies to divergent hantaviruses from geographically and phylogenetically distant rodent species.http://www.mdpi.com/1999-4915/6/5/2028Andes virusChileELISAstrip immunoblot assayhantavirus |
| spellingShingle | Karla Cautivo Tony Schountz Mariana Acuña-Retamar Marcela Ferrés Fernando Torres-Pérez Rapid Enzyme-Linked Immunosorbent Assay for the Detection of Hantavirus-Specific Antibodies in Divergent Small Mammals Viruses Andes virus Chile ELISA strip immunoblot assay hantavirus |
| title | Rapid Enzyme-Linked Immunosorbent Assay for the Detection of Hantavirus-Specific Antibodies in Divergent Small Mammals |
| title_full | Rapid Enzyme-Linked Immunosorbent Assay for the Detection of Hantavirus-Specific Antibodies in Divergent Small Mammals |
| title_fullStr | Rapid Enzyme-Linked Immunosorbent Assay for the Detection of Hantavirus-Specific Antibodies in Divergent Small Mammals |
| title_full_unstemmed | Rapid Enzyme-Linked Immunosorbent Assay for the Detection of Hantavirus-Specific Antibodies in Divergent Small Mammals |
| title_short | Rapid Enzyme-Linked Immunosorbent Assay for the Detection of Hantavirus-Specific Antibodies in Divergent Small Mammals |
| title_sort | rapid enzyme linked immunosorbent assay for the detection of hantavirus specific antibodies in divergent small mammals |
| topic | Andes virus Chile ELISA strip immunoblot assay hantavirus |
| url | http://www.mdpi.com/1999-4915/6/5/2028 |
| work_keys_str_mv | AT karlacautivo rapidenzymelinkedimmunosorbentassayforthedetectionofhantavirusspecificantibodiesindivergentsmallmammals AT tonyschountz rapidenzymelinkedimmunosorbentassayforthedetectionofhantavirusspecificantibodiesindivergentsmallmammals AT marianaacunaretamar rapidenzymelinkedimmunosorbentassayforthedetectionofhantavirusspecificantibodiesindivergentsmallmammals AT marcelaferres rapidenzymelinkedimmunosorbentassayforthedetectionofhantavirusspecificantibodiesindivergentsmallmammals AT fernandotorresperez rapidenzymelinkedimmunosorbentassayforthedetectionofhantavirusspecificantibodiesindivergentsmallmammals |