Adenosine Deaminase Inhibitory Activity of Medicinal Plants: Boost the Production of Cordycepin in <i>Cordyceps militaris</i>
Cordycepin, also known as 3′-deoxyadenosine, is a major active ingredient of <i>Cordyceps militaris</i> with diverse pharmacological effects. Due to its limited supply, many attempts have been conducted to enhance the cordycepin content. As part of this study, eight medicinal plants were...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-06-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/12/6/1260 |
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| author | Ayman Turk Solip Lee Sang Won Yeon Se Hwan Ryu Yoo Kyong Han Young Jun Kim Sung Min Ko Beom Seok Kim Bang Yeon Hwang Ki Yong Lee Mi Kyeong Lee |
| author_facet | Ayman Turk Solip Lee Sang Won Yeon Se Hwan Ryu Yoo Kyong Han Young Jun Kim Sung Min Ko Beom Seok Kim Bang Yeon Hwang Ki Yong Lee Mi Kyeong Lee |
| author_sort | Ayman Turk |
| collection | DOAJ |
| description | Cordycepin, also known as 3′-deoxyadenosine, is a major active ingredient of <i>Cordyceps militaris</i> with diverse pharmacological effects. Due to its limited supply, many attempts have been conducted to enhance the cordycepin content. As part of this study, eight medicinal plants were supplemented with cultivation substrates of <i>Cordyceps</i> to increase the cordycepin content. <i>Cordyceps</i> cultivated on brown rice supplemented with Mori Folium, Curcumae Rhizoma, Saururi Herba, and Angelicae Gigantis Radix exhibited increased cordycepin content compared to a brown rice control. Among them, the addition of 25% Mori Folium increased the cordycepin content up to 4 times. Adenosine deaminase (ADA) modulates the deamination of adenosine and deoxyadenosine, and the inhibitors have therapeutic potential with anti-proliferative and anti-inflammatory properties. As ADA is also known to be involved in converting cordycepin to 3′-deoxyinosine, the inhibitory activity of medicinal plants on ADA was measured by spectrophotometric analysis using cordycepin as a substrate. As expected, Mori Folium, Curcumae Rhizoma, Saururi Herba, and Angelicae Gigas Radix strongly inhibited ADA activity. Molecular docking analysis also showed the correlation between ADA and the major components of these medicinal plants. Conclusively, our research suggests a new strategy of using medicinal plants to enhance cordycepin production in <i>C. militaris</i>. |
| first_indexed | 2024-03-11T02:49:38Z |
| format | Article |
| id | doaj.art-5628925e9fbc439d9c71fdf03e7a0aca |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-11T02:49:38Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-5628925e9fbc439d9c71fdf03e7a0aca2023-11-18T09:03:42ZengMDPI AGAntioxidants2076-39212023-06-01126126010.3390/antiox12061260Adenosine Deaminase Inhibitory Activity of Medicinal Plants: Boost the Production of Cordycepin in <i>Cordyceps militaris</i>Ayman Turk0Solip Lee1Sang Won Yeon2Se Hwan Ryu3Yoo Kyong Han4Young Jun Kim5Sung Min Ko6Beom Seok Kim7Bang Yeon Hwang8Ki Yong Lee9Mi Kyeong Lee10College of Pharmacy, Chungbuk National University, Cheongju 28160, Republic of KoreaCollege of Pharmacy, Chungbuk National University, Cheongju 28160, Republic of KoreaCollege of Pharmacy, Chungbuk National University, Cheongju 28160, Republic of KoreaCollege of Pharmacy, Chungbuk National University, Cheongju 28160, Republic of KoreaCollege of Pharmacy, Korea University, Sejong 47236, Republic of KoreaCollege of Pharmacy, Korea University, Sejong 47236, Republic of KoreaCollege of Pharmacy, Chungbuk National University, Cheongju 28160, Republic of KoreaCollege of Pharmacy, Chungbuk National University, Cheongju 28160, Republic of KoreaCollege of Pharmacy, Chungbuk National University, Cheongju 28160, Republic of KoreaCollege of Pharmacy, Korea University, Sejong 47236, Republic of KoreaCollege of Pharmacy, Chungbuk National University, Cheongju 28160, Republic of KoreaCordycepin, also known as 3′-deoxyadenosine, is a major active ingredient of <i>Cordyceps militaris</i> with diverse pharmacological effects. Due to its limited supply, many attempts have been conducted to enhance the cordycepin content. As part of this study, eight medicinal plants were supplemented with cultivation substrates of <i>Cordyceps</i> to increase the cordycepin content. <i>Cordyceps</i> cultivated on brown rice supplemented with Mori Folium, Curcumae Rhizoma, Saururi Herba, and Angelicae Gigantis Radix exhibited increased cordycepin content compared to a brown rice control. Among them, the addition of 25% Mori Folium increased the cordycepin content up to 4 times. Adenosine deaminase (ADA) modulates the deamination of adenosine and deoxyadenosine, and the inhibitors have therapeutic potential with anti-proliferative and anti-inflammatory properties. As ADA is also known to be involved in converting cordycepin to 3′-deoxyinosine, the inhibitory activity of medicinal plants on ADA was measured by spectrophotometric analysis using cordycepin as a substrate. As expected, Mori Folium, Curcumae Rhizoma, Saururi Herba, and Angelicae Gigas Radix strongly inhibited ADA activity. Molecular docking analysis also showed the correlation between ADA and the major components of these medicinal plants. Conclusively, our research suggests a new strategy of using medicinal plants to enhance cordycepin production in <i>C. militaris</i>.https://www.mdpi.com/2076-3921/12/6/1260<i>Cordyceps militaris</i>Mori Foliumadenosine deaminase (ADA)cordycepinmedicinal plants |
| spellingShingle | Ayman Turk Solip Lee Sang Won Yeon Se Hwan Ryu Yoo Kyong Han Young Jun Kim Sung Min Ko Beom Seok Kim Bang Yeon Hwang Ki Yong Lee Mi Kyeong Lee Adenosine Deaminase Inhibitory Activity of Medicinal Plants: Boost the Production of Cordycepin in <i>Cordyceps militaris</i> Antioxidants <i>Cordyceps militaris</i> Mori Folium adenosine deaminase (ADA) cordycepin medicinal plants |
| title | Adenosine Deaminase Inhibitory Activity of Medicinal Plants: Boost the Production of Cordycepin in <i>Cordyceps militaris</i> |
| title_full | Adenosine Deaminase Inhibitory Activity of Medicinal Plants: Boost the Production of Cordycepin in <i>Cordyceps militaris</i> |
| title_fullStr | Adenosine Deaminase Inhibitory Activity of Medicinal Plants: Boost the Production of Cordycepin in <i>Cordyceps militaris</i> |
| title_full_unstemmed | Adenosine Deaminase Inhibitory Activity of Medicinal Plants: Boost the Production of Cordycepin in <i>Cordyceps militaris</i> |
| title_short | Adenosine Deaminase Inhibitory Activity of Medicinal Plants: Boost the Production of Cordycepin in <i>Cordyceps militaris</i> |
| title_sort | adenosine deaminase inhibitory activity of medicinal plants boost the production of cordycepin in i cordyceps militaris i |
| topic | <i>Cordyceps militaris</i> Mori Folium adenosine deaminase (ADA) cordycepin medicinal plants |
| url | https://www.mdpi.com/2076-3921/12/6/1260 |
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