Origanum vulgare ssp. vulgare: Chemical Composition and Biological Studies
The biological properties and main phenolic compounds of the O. vulgare L. ssp. vulgare extract are described in the present paper. The polyphenolic compounds were analyzed by chromatographic and spectrophotometric techniques. The antioxidant potential was evaluated using several methods: CUPRAC (cu...
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MDPI AG
2018-08-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/23/8/2077 |
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| author | Ilioara Oniga Cristina Pușcaș Radu Silaghi-Dumitrescu Neli-Kinga Olah Bogdan Sevastre Raluca Marica Ioan Marcus Alexandra Cristina Sevastre-Berghian Daniela Benedec Carmen Elena Pop Daniela Hanganu |
| author_facet | Ilioara Oniga Cristina Pușcaș Radu Silaghi-Dumitrescu Neli-Kinga Olah Bogdan Sevastre Raluca Marica Ioan Marcus Alexandra Cristina Sevastre-Berghian Daniela Benedec Carmen Elena Pop Daniela Hanganu |
| author_sort | Ilioara Oniga |
| collection | DOAJ |
| description | The biological properties and main phenolic compounds of the O. vulgare L. ssp. vulgare extract are described in the present paper. The polyphenolic compounds were analyzed by chromatographic and spectrophotometric techniques. The antioxidant potential was evaluated using several methods: CUPRAC (cupric ion reducing antioxidant capacity), FRAP (ferric reducing ability of plasma), inhibition of lipid peroxidation catalyzed by cytochrome c, and superoxide (SO) scavenging assays. The antimicrobial activity of the oregano extract was evaluated by means of agar-well diffusion assay. The hepatoprotective effect of the O. vulgare extract on CCl4-induced hepatotoxicity was evaluated in rats. Liver injury was estimated by determination of alanine aminotransaminase (ALT), aspartate aminotransaminase (AST), gamma-glutamyl transferase GGT, total protein and albumin concentrations, glutathione peroxidase (GPx), catalase (CAT), superoxide dismutase (SOD) and malondialdehyde (MDA). These values were improved by the administration of oregano extract. A specific phenolic profile was evidenced by these data, with large amounts of rosmarinic and chlorogenic acids. The oregano extract showed very strong antioxidant activity in good agreement with the phenolic content. Antimicrobial activity was good, especially against Salmonella enteritidis and Aspergillus niger strains. The high hepatoprotective, antioxidant and antimicrobial activity, along with polyphenol-rich content, can support the use of O. vulgare in therapy. We also expect our results to open new research directions for designing important new drug products, using indigenous plant material. |
| first_indexed | 2024-12-12T06:40:00Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-12T06:40:00Z |
| publishDate | 2018-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-562c0ea870d649df9394981edc00aa0a2022-12-22T00:34:22ZengMDPI AGMolecules1420-30492018-08-01238207710.3390/molecules23082077molecules23082077Origanum vulgare ssp. vulgare: Chemical Composition and Biological StudiesIlioara Oniga0Cristina Pușcaș1Radu Silaghi-Dumitrescu2Neli-Kinga Olah3Bogdan Sevastre4Raluca Marica5Ioan Marcus6Alexandra Cristina Sevastre-Berghian7Daniela Benedec8Carmen Elena Pop9Daniela Hanganu10Department of Pharmacognosy, Iuliu Hatieganu University of Medicine and Pharmacy, 12 I. Creanga Street, Cluj-Napoca 400010, RomaniaDepartment of Chemistry and Chemical Engineering, Babes-Bolyai University, 11 A. Janos Street, Cluj-Napoca 400028, RomaniaDepartment of Chemistry and Chemical Engineering, Babes-Bolyai University, 11 A. Janos Street, Cluj-Napoca 400028, RomaniaFaculty of Medicine, Pharmacy and Dental Medicine, Vasile Goldiş Western University of Arad, 86 L. Rebreanu Street, Arad 310414, RomaniaDepartment of Paraclinics, University of Agricultural Sciences and Veterinary Medicine, 3-5 Mănăştur Street, Cluj-Napoca 400372, RomaniaDepartment of Paraclinics, University of Agricultural Sciences and Veterinary Medicine, 3-5 Mănăştur Street, Cluj-Napoca 400372, RomaniaDepartment of Paraclinics, University of Agricultural Sciences and Veterinary Medicine, 3-5 Mănăştur Street, Cluj-Napoca 400372, RomaniaDepartment of Physiology, Iuliu Hatieganu University of Medicine and Pharmacy, 6 L. Pasteur Street, Cluj-Napoca, 400349, RomaniaDepartment of Pharmacognosy, Iuliu Hatieganu University of Medicine and Pharmacy, 12 I. Creanga Street, Cluj-Napoca 400010, RomaniaDepartment of Drug Industry and Biotechnology, Iuliu Hatieganu University of Medicine and Pharmacy, 12 I. Creanga Street, Cluj-Napoca 400010, RomaniaDepartment of Pharmacognosy, Iuliu Hatieganu University of Medicine and Pharmacy, 12 I. Creanga Street, Cluj-Napoca 400010, RomaniaThe biological properties and main phenolic compounds of the O. vulgare L. ssp. vulgare extract are described in the present paper. The polyphenolic compounds were analyzed by chromatographic and spectrophotometric techniques. The antioxidant potential was evaluated using several methods: CUPRAC (cupric ion reducing antioxidant capacity), FRAP (ferric reducing ability of plasma), inhibition of lipid peroxidation catalyzed by cytochrome c, and superoxide (SO) scavenging assays. The antimicrobial activity of the oregano extract was evaluated by means of agar-well diffusion assay. The hepatoprotective effect of the O. vulgare extract on CCl4-induced hepatotoxicity was evaluated in rats. Liver injury was estimated by determination of alanine aminotransaminase (ALT), aspartate aminotransaminase (AST), gamma-glutamyl transferase GGT, total protein and albumin concentrations, glutathione peroxidase (GPx), catalase (CAT), superoxide dismutase (SOD) and malondialdehyde (MDA). These values were improved by the administration of oregano extract. A specific phenolic profile was evidenced by these data, with large amounts of rosmarinic and chlorogenic acids. The oregano extract showed very strong antioxidant activity in good agreement with the phenolic content. Antimicrobial activity was good, especially against Salmonella enteritidis and Aspergillus niger strains. The high hepatoprotective, antioxidant and antimicrobial activity, along with polyphenol-rich content, can support the use of O. vulgare in therapy. We also expect our results to open new research directions for designing important new drug products, using indigenous plant material.http://www.mdpi.com/1420-3049/23/8/2077Origanum vulgarepolyphenolscythocrome cantioxidantantimicrobialhepatoprotective |
| spellingShingle | Ilioara Oniga Cristina Pușcaș Radu Silaghi-Dumitrescu Neli-Kinga Olah Bogdan Sevastre Raluca Marica Ioan Marcus Alexandra Cristina Sevastre-Berghian Daniela Benedec Carmen Elena Pop Daniela Hanganu Origanum vulgare ssp. vulgare: Chemical Composition and Biological Studies Molecules Origanum vulgare polyphenols cythocrome c antioxidant antimicrobial hepatoprotective |
| title | Origanum vulgare ssp. vulgare: Chemical Composition and Biological Studies |
| title_full | Origanum vulgare ssp. vulgare: Chemical Composition and Biological Studies |
| title_fullStr | Origanum vulgare ssp. vulgare: Chemical Composition and Biological Studies |
| title_full_unstemmed | Origanum vulgare ssp. vulgare: Chemical Composition and Biological Studies |
| title_short | Origanum vulgare ssp. vulgare: Chemical Composition and Biological Studies |
| title_sort | origanum vulgare ssp vulgare chemical composition and biological studies |
| topic | Origanum vulgare polyphenols cythocrome c antioxidant antimicrobial hepatoprotective |
| url | http://www.mdpi.com/1420-3049/23/8/2077 |
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