Genetic and physiological variation in two strains of Japanese quail
Abstract Background Detecting the genetic and physiological variations in two Japanese quail strains could be used to suggest a new avian model for future breeding studies. Consequently, two estimations were performed on two Japanese quail strains: gray quail strain (GJQS) and white jumbo quail stra...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-01-01
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| Series: | Journal of Genetic Engineering and Biotechnology |
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| Online Access: | https://doi.org/10.1186/s43141-020-00100-3 |
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| author | Nashat Saeid Ibrahim Mohammed Ahmed El-Sayed Heba Abdelwahab Mahmoud Assi Ahmed Enab Abdel-Moneim Eid Abdel-Moneim |
| author_facet | Nashat Saeid Ibrahim Mohammed Ahmed El-Sayed Heba Abdelwahab Mahmoud Assi Ahmed Enab Abdel-Moneim Eid Abdel-Moneim |
| author_sort | Nashat Saeid Ibrahim |
| collection | DOAJ |
| description | Abstract Background Detecting the genetic and physiological variations in two Japanese quail strains could be used to suggest a new avian model for future breeding studies. Consequently, two estimations were performed on two Japanese quail strains: gray quail strain (GJQS) and white jumbo quail strain (WJQS). The first estimation was conducted on carcass characteristics, breast muscles, breast concentration of collagen type I, and body measurements. In contrast, blood samples were collected for the second estimation for genomic DNA extraction and genetic analysis. Results A total of 62 alleles out of 97 specific alleles (63.92%) were detected overall loci (14 microsatellite loci) for the two strains. A total of 27 specific alleles of WJQS were observed, and 35 were obtained for GJQS. The percentage of similarity was 48.09% ranged from 4.35 with UBC001 to 100% with GUJ0051. WJQS had greater body weights and a higher value of pectoral muscle and supracoracoideus muscle than GJQS. The breast muscles of GJQS exhibited a higher concentration of type I collagen than the WJQS. Furthermore, males showed higher concentrations of collagen type I than females. WJQS showed a higher body length, chest girth, chest length, thigh length, thigh girth, drumstick length, and drumstick girth (cm) than GJQS. WJQS showed more significant differences in carcass traits compared with GJQS. Conclusion The physiological differences between WJQS and GJQS were ascertained with microsatellite markers, which indicated high polymorphism between these strains. These observations provided a scientific basis for evaluating and utilizing the genetic resources of WJQS and GJQS in a future genetic improvement program. |
| first_indexed | 2024-04-24T08:24:40Z |
| format | Article |
| id | doaj.art-562cb50ab59a49d4aa8c48ac66d19e07 |
| institution | Directory Open Access Journal |
| issn | 2090-5920 |
| language | English |
| last_indexed | 2024-04-24T08:24:40Z |
| publishDate | 2021-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Genetic Engineering and Biotechnology |
| spelling | doaj.art-562cb50ab59a49d4aa8c48ac66d19e072024-04-16T23:20:21ZengElsevierJournal of Genetic Engineering and Biotechnology2090-59202021-01-0119111210.1186/s43141-020-00100-3Genetic and physiological variation in two strains of Japanese quailNashat Saeid Ibrahim0Mohammed Ahmed El-Sayed1Heba Abdelwahab Mahmoud Assi2Ahmed Enab3Abdel-Moneim Eid Abdel-Moneim4Biological Application Department, Nuclear Research Center, Egyptian Atomic Energy AuthorityNational Gene Bank, Animal Genetic Resources Department, Agricultural Research CenterAnimal Production Research Institute, Agricultural Research CenterDepartment of Poultry and Fish Production, Faculty of Agriculture, Menoufia UniversityBiological Application Department, Nuclear Research Center, Egyptian Atomic Energy AuthorityAbstract Background Detecting the genetic and physiological variations in two Japanese quail strains could be used to suggest a new avian model for future breeding studies. Consequently, two estimations were performed on two Japanese quail strains: gray quail strain (GJQS) and white jumbo quail strain (WJQS). The first estimation was conducted on carcass characteristics, breast muscles, breast concentration of collagen type I, and body measurements. In contrast, blood samples were collected for the second estimation for genomic DNA extraction and genetic analysis. Results A total of 62 alleles out of 97 specific alleles (63.92%) were detected overall loci (14 microsatellite loci) for the two strains. A total of 27 specific alleles of WJQS were observed, and 35 were obtained for GJQS. The percentage of similarity was 48.09% ranged from 4.35 with UBC001 to 100% with GUJ0051. WJQS had greater body weights and a higher value of pectoral muscle and supracoracoideus muscle than GJQS. The breast muscles of GJQS exhibited a higher concentration of type I collagen than the WJQS. Furthermore, males showed higher concentrations of collagen type I than females. WJQS showed a higher body length, chest girth, chest length, thigh length, thigh girth, drumstick length, and drumstick girth (cm) than GJQS. WJQS showed more significant differences in carcass traits compared with GJQS. Conclusion The physiological differences between WJQS and GJQS were ascertained with microsatellite markers, which indicated high polymorphism between these strains. These observations provided a scientific basis for evaluating and utilizing the genetic resources of WJQS and GJQS in a future genetic improvement program.https://doi.org/10.1186/s43141-020-00100-3Japanese quail strainGenetic and physiological variationMicrosatellite markers |
| spellingShingle | Nashat Saeid Ibrahim Mohammed Ahmed El-Sayed Heba Abdelwahab Mahmoud Assi Ahmed Enab Abdel-Moneim Eid Abdel-Moneim Genetic and physiological variation in two strains of Japanese quail Journal of Genetic Engineering and Biotechnology Japanese quail strain Genetic and physiological variation Microsatellite markers |
| title | Genetic and physiological variation in two strains of Japanese quail |
| title_full | Genetic and physiological variation in two strains of Japanese quail |
| title_fullStr | Genetic and physiological variation in two strains of Japanese quail |
| title_full_unstemmed | Genetic and physiological variation in two strains of Japanese quail |
| title_short | Genetic and physiological variation in two strains of Japanese quail |
| title_sort | genetic and physiological variation in two strains of japanese quail |
| topic | Japanese quail strain Genetic and physiological variation Microsatellite markers |
| url | https://doi.org/10.1186/s43141-020-00100-3 |
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