The role of cyclic nucleotide phosphodiesterases in the regulation of adipocyte lipolysis
This study assessed the effects of selective inhibitors of 3′,5′-cyclic nucleotide phosphodiesterases (PDEs) on adipocyte lipolysis. IC224, a selective inhibitor of type 1 phosphodiesterase (PDE1), suppressed lipolysis in murine 3T3-L1 adipocytes (69.6 ± 5.4% of vehicle control) but had no effect in...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2005-03-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520340347 |
| _version_ | 1818739299190833152 |
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| author | Peter B. Snyder James M. Esselstyn Kate Loughney Sharon L. Wolda Vincent A. Florio |
| author_facet | Peter B. Snyder James M. Esselstyn Kate Loughney Sharon L. Wolda Vincent A. Florio |
| author_sort | Peter B. Snyder |
| collection | DOAJ |
| description | This study assessed the effects of selective inhibitors of 3′,5′-cyclic nucleotide phosphodiesterases (PDEs) on adipocyte lipolysis. IC224, a selective inhibitor of type 1 phosphodiesterase (PDE1), suppressed lipolysis in murine 3T3-L1 adipocytes (69.6 ± 5.4% of vehicle control) but had no effect in human adipocytes. IC933, a selective inhibitor of PDE2, had no effect on lipolysis in either cultured murine 3T3-L1 adipocytes or human adipocytes. Inhibition of PDE3 with cilostamide moderately stimulated lipolysis in murine 3T3-L1 and rat adipocytes (397 ± 25% and 235 ± 26% of control, respectively) and markedly stimulated lipolysis in human adipocytes (932 ± 7.6% of control). Inhibition of PDE4 with rolipram moderately stimulated lipolysis in murine 3T3-L1 adipocytes (291 ± 13% of control) and weakly stimulated lipolysis in rat adipocytes (149 ± 7.0% of control) but had no effect on lipolysis in human adipocytes. Cultured adipocytes also responded differently to a combination of PDE3 and PDE4 inhibitors. Simultaneous exposure to cilostamide and rolipram had a synergistic effect on lipolysis in murine 3T3-L1 and rat adipocytes but not in human adipocytes.Hence, the relative importance of PDE3 and PDE4 in regulating lipolysis differed in cultured murine, rat, and human adipocytes. |
| first_indexed | 2024-12-18T01:22:37Z |
| format | Article |
| id | doaj.art-562ce4ad18764bbebb45e15e2e676b40 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-18T01:22:37Z |
| publishDate | 2005-03-01 |
| publisher | Elsevier |
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| series | Journal of Lipid Research |
| spelling | doaj.art-562ce4ad18764bbebb45e15e2e676b402022-12-21T21:25:47ZengElsevierJournal of Lipid Research0022-22752005-03-01463494503The role of cyclic nucleotide phosphodiesterases in the regulation of adipocyte lipolysisPeter B. Snyder0James M. Esselstyn1Kate Loughney2Sharon L. Wolda3Vincent A. Florio4ICOS Corporation, Bothell, WA 98021ICOS Corporation, Bothell, WA 98021ICOS Corporation, Bothell, WA 98021ICOS Corporation, Bothell, WA 98021ICOS Corporation, Bothell, WA 98021This study assessed the effects of selective inhibitors of 3′,5′-cyclic nucleotide phosphodiesterases (PDEs) on adipocyte lipolysis. IC224, a selective inhibitor of type 1 phosphodiesterase (PDE1), suppressed lipolysis in murine 3T3-L1 adipocytes (69.6 ± 5.4% of vehicle control) but had no effect in human adipocytes. IC933, a selective inhibitor of PDE2, had no effect on lipolysis in either cultured murine 3T3-L1 adipocytes or human adipocytes. Inhibition of PDE3 with cilostamide moderately stimulated lipolysis in murine 3T3-L1 and rat adipocytes (397 ± 25% and 235 ± 26% of control, respectively) and markedly stimulated lipolysis in human adipocytes (932 ± 7.6% of control). Inhibition of PDE4 with rolipram moderately stimulated lipolysis in murine 3T3-L1 adipocytes (291 ± 13% of control) and weakly stimulated lipolysis in rat adipocytes (149 ± 7.0% of control) but had no effect on lipolysis in human adipocytes. Cultured adipocytes also responded differently to a combination of PDE3 and PDE4 inhibitors. Simultaneous exposure to cilostamide and rolipram had a synergistic effect on lipolysis in murine 3T3-L1 and rat adipocytes but not in human adipocytes.Hence, the relative importance of PDE3 and PDE4 in regulating lipolysis differed in cultured murine, rat, and human adipocytes.http://www.sciencedirect.com/science/article/pii/S0022227520340347cilostamiderolipramtriglyceride |
| spellingShingle | Peter B. Snyder James M. Esselstyn Kate Loughney Sharon L. Wolda Vincent A. Florio The role of cyclic nucleotide phosphodiesterases in the regulation of adipocyte lipolysis Journal of Lipid Research cilostamide rolipram triglyceride |
| title | The role of cyclic nucleotide phosphodiesterases in the regulation of adipocyte lipolysis |
| title_full | The role of cyclic nucleotide phosphodiesterases in the regulation of adipocyte lipolysis |
| title_fullStr | The role of cyclic nucleotide phosphodiesterases in the regulation of adipocyte lipolysis |
| title_full_unstemmed | The role of cyclic nucleotide phosphodiesterases in the regulation of adipocyte lipolysis |
| title_short | The role of cyclic nucleotide phosphodiesterases in the regulation of adipocyte lipolysis |
| title_sort | role of cyclic nucleotide phosphodiesterases in the regulation of adipocyte lipolysis |
| topic | cilostamide rolipram triglyceride |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520340347 |
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