Biochemical Marker Assessment of Chronic Carbamazepine Exposure at Environmentally Relevant Concentrations in Juvenile Common Carp (<i>Cyprinus carpio</i>)
Worldwide, the anticonvulsant drug carbamazepine (CBZ) is the most frequently identified pharmaceutical residue detected in rivers. Reported chronic effects of CBZ in non-target freshwater organisms, particularly fish, include oxidative stress and damage to liver tissues. Studies on CBZ effects in f...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/6/1136 |
| _version_ | 1827662819390128128 |
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| author | Xinyue Liang Zsolt Csenki Bence Ivánovics Illés Bock Balázs Csorbai József Molnár Erna Vásárhelyi Jeffrey Griffitts Árpád Ferincz Béla Urbányi András Ács |
| author_facet | Xinyue Liang Zsolt Csenki Bence Ivánovics Illés Bock Balázs Csorbai József Molnár Erna Vásárhelyi Jeffrey Griffitts Árpád Ferincz Béla Urbányi András Ács |
| author_sort | Xinyue Liang |
| collection | DOAJ |
| description | Worldwide, the anticonvulsant drug carbamazepine (CBZ) is the most frequently identified pharmaceutical residue detected in rivers. Reported chronic effects of CBZ in non-target freshwater organisms, particularly fish, include oxidative stress and damage to liver tissues. Studies on CBZ effects in fish are mostly limited to zebrafish and rainbow trout studies. Furthermore, there are only a few chronic CBZ studies using near environmental concentrations. In this study, we provide data on subacute effects of CBZ exposure (28 days) to common carp (<i>Cyprinus carpio</i>), employing a set of biochemical markers of damage and exposure. CBZ was found to induce a significant change in the hepatic antioxidant status of fish subjected to 5 µg/L. Moreover, with increasing concentrations, enzymatic and non-enzymatic biomarkers of oxidative defence (catalase (CAT), superoxide dismutase (SOD), glutathione reductase (GR), DNA strand breaks)), toxicant biotransformation (ethoxyresorufin-o-demethylase (EROD), glutathione-S-transferase (GST)), and organ and tissue damage (lactate dehydrogenase (LDH), cetylcholinesterase (AChE)) were altered. The AChE, LDH, and lipid peroxidation (LPO) results indicate the occurrence of apoptotic process activation and tissue damage after 28 days of exposure to CBZ. These findings suggest significant adverse effects of CBZ exposure to common carp at concentrations often found in surface waters. |
| first_indexed | 2024-03-10T00:34:22Z |
| format | Article |
| id | doaj.art-562e367206374d5c8c74bfdb84d1f88b |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T00:34:22Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-562e367206374d5c8c74bfdb84d1f88b2023-11-23T15:20:13ZengMDPI AGAntioxidants2076-39212022-06-01116113610.3390/antiox11061136Biochemical Marker Assessment of Chronic Carbamazepine Exposure at Environmentally Relevant Concentrations in Juvenile Common Carp (<i>Cyprinus carpio</i>)Xinyue Liang0Zsolt Csenki1Bence Ivánovics2Illés Bock3Balázs Csorbai4József Molnár5Erna Vásárhelyi6Jeffrey Griffitts7Árpád Ferincz8Béla Urbányi9András Ács10Department of Freshwater Fish Ecology, Hungarian University of Agriculture and Life Sciences, Páter Károly u. 1., H-2100 Gödöllő, HungaryDepartment of Environmental Toxicology, Institute of Aquaculture and Environmental Safety, Hungarian University of Agriculture and Life Sciences, Páter Károly u. 1., H-2100 Gödöllő, HungaryDepartment of Environmental Toxicology, Institute of Aquaculture and Environmental Safety, Hungarian University of Agriculture and Life Sciences, Páter Károly u. 1., H-2100 Gödöllő, HungaryDepartment of Environmental Toxicology, Institute of Aquaculture and Environmental Safety, Hungarian University of Agriculture and Life Sciences, Páter Károly u. 1., H-2100 Gödöllő, HungaryDepartment of Aquaculture, Hungarian University of Agriculture and Life Sciences, Páter Károly u. 1., H-2100 Gödöllő, HungaryDepartment of Aquaculture, Hungarian University of Agriculture and Life Sciences, Páter Károly u. 1., H-2100 Gödöllő, HungaryDepartment of Environmental Toxicology, Institute of Aquaculture and Environmental Safety, Hungarian University of Agriculture and Life Sciences, Páter Károly u. 1., H-2100 Gödöllő, HungaryDepartment of Environmental Toxicology, Institute of Aquaculture and Environmental Safety, Hungarian University of Agriculture and Life Sciences, Páter Károly u. 1., H-2100 Gödöllő, HungaryDepartment of Freshwater Fish Ecology, Hungarian University of Agriculture and Life Sciences, Páter Károly u. 1., H-2100 Gödöllő, HungaryDepartment of Aquaculture, Hungarian University of Agriculture and Life Sciences, Páter Károly u. 1., H-2100 Gödöllő, HungaryDepartment of Freshwater Fish Ecology, Hungarian University of Agriculture and Life Sciences, Páter Károly u. 1., H-2100 Gödöllő, HungaryWorldwide, the anticonvulsant drug carbamazepine (CBZ) is the most frequently identified pharmaceutical residue detected in rivers. Reported chronic effects of CBZ in non-target freshwater organisms, particularly fish, include oxidative stress and damage to liver tissues. Studies on CBZ effects in fish are mostly limited to zebrafish and rainbow trout studies. Furthermore, there are only a few chronic CBZ studies using near environmental concentrations. In this study, we provide data on subacute effects of CBZ exposure (28 days) to common carp (<i>Cyprinus carpio</i>), employing a set of biochemical markers of damage and exposure. CBZ was found to induce a significant change in the hepatic antioxidant status of fish subjected to 5 µg/L. Moreover, with increasing concentrations, enzymatic and non-enzymatic biomarkers of oxidative defence (catalase (CAT), superoxide dismutase (SOD), glutathione reductase (GR), DNA strand breaks)), toxicant biotransformation (ethoxyresorufin-o-demethylase (EROD), glutathione-S-transferase (GST)), and organ and tissue damage (lactate dehydrogenase (LDH), cetylcholinesterase (AChE)) were altered. The AChE, LDH, and lipid peroxidation (LPO) results indicate the occurrence of apoptotic process activation and tissue damage after 28 days of exposure to CBZ. These findings suggest significant adverse effects of CBZ exposure to common carp at concentrations often found in surface waters.https://www.mdpi.com/2076-3921/11/6/1136carbamazepineoxidative stressfish biomarkercommon carp (<i>Cyprinus carpio</i>)chronic effects |
| spellingShingle | Xinyue Liang Zsolt Csenki Bence Ivánovics Illés Bock Balázs Csorbai József Molnár Erna Vásárhelyi Jeffrey Griffitts Árpád Ferincz Béla Urbányi András Ács Biochemical Marker Assessment of Chronic Carbamazepine Exposure at Environmentally Relevant Concentrations in Juvenile Common Carp (<i>Cyprinus carpio</i>) Antioxidants carbamazepine oxidative stress fish biomarker common carp (<i>Cyprinus carpio</i>) chronic effects |
| title | Biochemical Marker Assessment of Chronic Carbamazepine Exposure at Environmentally Relevant Concentrations in Juvenile Common Carp (<i>Cyprinus carpio</i>) |
| title_full | Biochemical Marker Assessment of Chronic Carbamazepine Exposure at Environmentally Relevant Concentrations in Juvenile Common Carp (<i>Cyprinus carpio</i>) |
| title_fullStr | Biochemical Marker Assessment of Chronic Carbamazepine Exposure at Environmentally Relevant Concentrations in Juvenile Common Carp (<i>Cyprinus carpio</i>) |
| title_full_unstemmed | Biochemical Marker Assessment of Chronic Carbamazepine Exposure at Environmentally Relevant Concentrations in Juvenile Common Carp (<i>Cyprinus carpio</i>) |
| title_short | Biochemical Marker Assessment of Chronic Carbamazepine Exposure at Environmentally Relevant Concentrations in Juvenile Common Carp (<i>Cyprinus carpio</i>) |
| title_sort | biochemical marker assessment of chronic carbamazepine exposure at environmentally relevant concentrations in juvenile common carp i cyprinus carpio i |
| topic | carbamazepine oxidative stress fish biomarker common carp (<i>Cyprinus carpio</i>) chronic effects |
| url | https://www.mdpi.com/2076-3921/11/6/1136 |
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