Development of Terahertz Time-Domain Spectroscopy for Properties Analysis of Highly Diluted Antibodies
This work represents the development of a three-band (Debye relaxation, intermolecular bending, and stretching vibrational bands) approach to the terahertz time-domain spectroscopy (THz-TDS) for the analysis of aqueous solutions of lactose saturated with highly diluted (HD) antibodies to interferon-...
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| Format: | Article |
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MDPI AG
2020-10-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/10/21/7736 |
| _version_ | 1797549103268233216 |
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| author | Nikita Penkov Eugeny Fesenko |
| author_facet | Nikita Penkov Eugeny Fesenko |
| author_sort | Nikita Penkov |
| collection | DOAJ |
| description | This work represents the development of a three-band (Debye relaxation, intermolecular bending, and stretching vibrational bands) approach to the terahertz time-domain spectroscopy (THz-TDS) for the analysis of aqueous solutions of lactose saturated with highly diluted (HD) antibodies to interferon-gamma. Since THz-TDS is sensitive to a very slight changes in water properties, we aimed to assess its applicability for the analysis of HD antibodies. To evaluate water features in tested samples we also used Fourier transform infrared spectroscopy, considering the first overtone of intramolecular stretching vibrations of water molecules. We showed a broadening of the energy distribution of intermolecular bonds in the solution of HD substances in comparison to the control solution. This broadening corresponded to the presence of an active component even in HD samples. The results of both methods were similar; however, with the THz-TDS it was possible to analyze the changes in energy distribution of intermolecular (hydrogen) bonds in the solutions. Therefore, our approach to THz-TDS method is applicable for analyzing structural properties of the solutions containing HD antibodies and for the indirect investigation of altered aqueous properties in water solutions when direct measurements of a substance concentration are not available. |
| first_indexed | 2024-03-10T15:09:48Z |
| format | Article |
| id | doaj.art-563089019d9043d7be689f57800a4cec |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T15:09:48Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-563089019d9043d7be689f57800a4cec2023-11-20T19:25:22ZengMDPI AGApplied Sciences2076-34172020-10-011021773610.3390/app10217736Development of Terahertz Time-Domain Spectroscopy for Properties Analysis of Highly Diluted AntibodiesNikita Penkov0Eugeny Fesenko1Institute of Cell Biophysics RAS, Federal Research Center Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences, Institutskaya str. 3, 142290 Pushchino, RussiaInstitute of Cell Biophysics RAS, Federal Research Center Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences, Institutskaya str. 3, 142290 Pushchino, RussiaThis work represents the development of a three-band (Debye relaxation, intermolecular bending, and stretching vibrational bands) approach to the terahertz time-domain spectroscopy (THz-TDS) for the analysis of aqueous solutions of lactose saturated with highly diluted (HD) antibodies to interferon-gamma. Since THz-TDS is sensitive to a very slight changes in water properties, we aimed to assess its applicability for the analysis of HD antibodies. To evaluate water features in tested samples we also used Fourier transform infrared spectroscopy, considering the first overtone of intramolecular stretching vibrations of water molecules. We showed a broadening of the energy distribution of intermolecular bonds in the solution of HD substances in comparison to the control solution. This broadening corresponded to the presence of an active component even in HD samples. The results of both methods were similar; however, with the THz-TDS it was possible to analyze the changes in energy distribution of intermolecular (hydrogen) bonds in the solutions. Therefore, our approach to THz-TDS method is applicable for analyzing structural properties of the solutions containing HD antibodies and for the indirect investigation of altered aqueous properties in water solutions when direct measurements of a substance concentration are not available.https://www.mdpi.com/2076-3417/10/21/7736terahertz spectroscopyTHz-TDShigh dilutionssolution structuredynamics of water molecules |
| spellingShingle | Nikita Penkov Eugeny Fesenko Development of Terahertz Time-Domain Spectroscopy for Properties Analysis of Highly Diluted Antibodies Applied Sciences terahertz spectroscopy THz-TDS high dilutions solution structure dynamics of water molecules |
| title | Development of Terahertz Time-Domain Spectroscopy for Properties Analysis of Highly Diluted Antibodies |
| title_full | Development of Terahertz Time-Domain Spectroscopy for Properties Analysis of Highly Diluted Antibodies |
| title_fullStr | Development of Terahertz Time-Domain Spectroscopy for Properties Analysis of Highly Diluted Antibodies |
| title_full_unstemmed | Development of Terahertz Time-Domain Spectroscopy for Properties Analysis of Highly Diluted Antibodies |
| title_short | Development of Terahertz Time-Domain Spectroscopy for Properties Analysis of Highly Diluted Antibodies |
| title_sort | development of terahertz time domain spectroscopy for properties analysis of highly diluted antibodies |
| topic | terahertz spectroscopy THz-TDS high dilutions solution structure dynamics of water molecules |
| url | https://www.mdpi.com/2076-3417/10/21/7736 |
| work_keys_str_mv | AT nikitapenkov developmentofterahertztimedomainspectroscopyforpropertiesanalysisofhighlydilutedantibodies AT eugenyfesenko developmentofterahertztimedomainspectroscopyforpropertiesanalysisofhighlydilutedantibodies |