Biodegradable chitin conduit tubulation combined with bone marrow mesenchymal stem cell transplantation for treatment of spinal cord injury by reducing glial scar and cavity formation
We examined the restorative effect of modified biodegradable chitin conduits in combination with bone marrow mesenchymal stem cell transplantation after right spinal cord hemisection injury. Immunohistochemical staining revealed that biological conduit sleeve bridging reduced glial scar formation an...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2015-01-01
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| Series: | Neural Regeneration Research |
| Subjects: | |
| Online Access: | http://www.nrronline.org/article.asp?issn=1673-5374;year=2015;volume=10;issue=1;spage=104;epage=111;aulast=Xue |
| _version_ | 1819004481340178432 |
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| author | Feng Xue Er-jun Wu Pei-xun Zhang Li-ya A Yu-hui Kou Xiao-feng Yin Na Han |
| author_facet | Feng Xue Er-jun Wu Pei-xun Zhang Li-ya A Yu-hui Kou Xiao-feng Yin Na Han |
| author_sort | Feng Xue |
| collection | DOAJ |
| description | We examined the restorative effect of modified biodegradable chitin conduits in combination with bone marrow mesenchymal stem cell transplantation after right spinal cord hemisection injury. Immunohistochemical staining revealed that biological conduit sleeve bridging reduced glial scar formation and spinal muscular atrophy after spinal cord hemisection. Bone marrow mesenchymal stem cells survived and proliferated after transplantation in vivo, and differentiated into cells double-positive for S100 (Schwann cell marker) and glial fibrillary acidic protein (glial cell marker) at 8 weeks. Retrograde tracing showed that more nerve fibers had grown through the injured spinal cord at 14 weeks after combination therapy than either treatment alone. Our findings indicate that a biological conduit combined with bone marrow mesenchymal stem cell transplantation effectively prevented scar formation and provided a favorable local microenvironment for the proliferation, migration and differentiation of bone marrow mesenchymal stem cells in the spinal cord, thus promoting restoration following spinal cord hemisection injury. |
| first_indexed | 2024-12-20T23:37:35Z |
| format | Article |
| id | doaj.art-5633547f481b4547bc1f291befc9101c |
| institution | Directory Open Access Journal |
| issn | 1673-5374 1876-7958 |
| language | English |
| last_indexed | 2024-12-20T23:37:35Z |
| publishDate | 2015-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Neural Regeneration Research |
| spelling | doaj.art-5633547f481b4547bc1f291befc9101c2022-12-21T19:23:10ZengWolters Kluwer Medknow PublicationsNeural Regeneration Research1673-53741876-79582015-01-0110110411110.4103/1673-5374.150715Biodegradable chitin conduit tubulation combined with bone marrow mesenchymal stem cell transplantation for treatment of spinal cord injury by reducing glial scar and cavity formationFeng XueEr-jun WuPei-xun ZhangLi-ya AYu-hui KouXiao-feng YinNa HanWe examined the restorative effect of modified biodegradable chitin conduits in combination with bone marrow mesenchymal stem cell transplantation after right spinal cord hemisection injury. Immunohistochemical staining revealed that biological conduit sleeve bridging reduced glial scar formation and spinal muscular atrophy after spinal cord hemisection. Bone marrow mesenchymal stem cells survived and proliferated after transplantation in vivo, and differentiated into cells double-positive for S100 (Schwann cell marker) and glial fibrillary acidic protein (glial cell marker) at 8 weeks. Retrograde tracing showed that more nerve fibers had grown through the injured spinal cord at 14 weeks after combination therapy than either treatment alone. Our findings indicate that a biological conduit combined with bone marrow mesenchymal stem cell transplantation effectively prevented scar formation and provided a favorable local microenvironment for the proliferation, migration and differentiation of bone marrow mesenchymal stem cells in the spinal cord, thus promoting restoration following spinal cord hemisection injury.http://www.nrronline.org/article.asp?issn=1673-5374;year=2015;volume=10;issue=1;spage=104;epage=111;aulast=Xuenerve regeneration; spinal cord injury; spinal cord hemisection; biological conduit; bone marrow mesenchymal stem cells; stem cells; transmission electron microscope; cell transplantation; neurons; nerve fibers; NSFC grants; neural regeneration |
| spellingShingle | Feng Xue Er-jun Wu Pei-xun Zhang Li-ya A Yu-hui Kou Xiao-feng Yin Na Han Biodegradable chitin conduit tubulation combined with bone marrow mesenchymal stem cell transplantation for treatment of spinal cord injury by reducing glial scar and cavity formation Neural Regeneration Research nerve regeneration; spinal cord injury; spinal cord hemisection; biological conduit; bone marrow mesenchymal stem cells; stem cells; transmission electron microscope; cell transplantation; neurons; nerve fibers; NSFC grants; neural regeneration |
| title | Biodegradable chitin conduit tubulation combined with bone marrow mesenchymal stem cell transplantation for treatment of spinal cord injury by reducing glial scar and cavity formation |
| title_full | Biodegradable chitin conduit tubulation combined with bone marrow mesenchymal stem cell transplantation for treatment of spinal cord injury by reducing glial scar and cavity formation |
| title_fullStr | Biodegradable chitin conduit tubulation combined with bone marrow mesenchymal stem cell transplantation for treatment of spinal cord injury by reducing glial scar and cavity formation |
| title_full_unstemmed | Biodegradable chitin conduit tubulation combined with bone marrow mesenchymal stem cell transplantation for treatment of spinal cord injury by reducing glial scar and cavity formation |
| title_short | Biodegradable chitin conduit tubulation combined with bone marrow mesenchymal stem cell transplantation for treatment of spinal cord injury by reducing glial scar and cavity formation |
| title_sort | biodegradable chitin conduit tubulation combined with bone marrow mesenchymal stem cell transplantation for treatment of spinal cord injury by reducing glial scar and cavity formation |
| topic | nerve regeneration; spinal cord injury; spinal cord hemisection; biological conduit; bone marrow mesenchymal stem cells; stem cells; transmission electron microscope; cell transplantation; neurons; nerve fibers; NSFC grants; neural regeneration |
| url | http://www.nrronline.org/article.asp?issn=1673-5374;year=2015;volume=10;issue=1;spage=104;epage=111;aulast=Xue |
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