Occurrence of <i>bla<sub>TEM</sub></i> and <i>bla<sub>CTXM</sub></i> Genes and Biofilm-Forming Ability among Clinical Isolates of <i>Pseudomonas aeruginosa</i> and <i>Acinetobacter baumannii</i> in Yaoundé, Cameroon
Background: <i>Pseudomonas aeruginosa</i> (PSA) and <i>Acinetobacter baumannii</i> (ACB) are non-fermentative bacteria mostly associated with nosocomial infections in humans. Objective: This study aimed to determine the antimicrobial resistance profiles and virulence gene of...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-05-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/8/5/708 |
| _version_ | 1797568293751488512 |
|---|---|
| author | Estelle Longla Madaha Hortense Kamga Gonsu Rhoda Nsen Bughe Marie Christine Fonkoua Collins Njie Ateba Wilfred Fon Mbacham |
| author_facet | Estelle Longla Madaha Hortense Kamga Gonsu Rhoda Nsen Bughe Marie Christine Fonkoua Collins Njie Ateba Wilfred Fon Mbacham |
| author_sort | Estelle Longla Madaha |
| collection | DOAJ |
| description | Background: <i>Pseudomonas aeruginosa</i> (PSA) and <i>Acinetobacter baumannii</i> (ACB) are non-fermentative bacteria mostly associated with nosocomial infections in humans. Objective: This study aimed to determine the antimicrobial resistance profiles and virulence gene of PSA and ACB previously isolated from humans in selected health facilities in Yaoundé, Cameroon. Methods: A total of 77 and 27 presumptive PSA and ACB isolates, respectively, were collected from the Yaoundé teaching hospital. These isolates were previously isolated from various samples including pus, blood and broncho-alveolar lavage. The identities of the isolates were determined through polymerase chain reaction (PCR) amplification of PSA and ACB specific sequences. Antimicrobial susceptibility testing (AST) was performed using the Kirby–Bauer disc diffusion method. Phenotypical expression of AmpC β-lactamases (<i>Amp</i>C), extended spectrum β-lactamases (ESBLs) and metallo β-Lactamases (MBLs) were determined using the combined disc method. Bacterial genomes were screened for the presence of β-lactamases <i>bla<sub>TEM</sub></i> and <i>bla<sub>CTXM</sub></i> genes using specific PCR. The pathogenicity of PSA and ACB was assessed through amplification of the <i>lasB, exoA, pslA</i> and <i>exoS</i> as well as <i>OmpA</i> and <i>csuE</i> virulence genes, respectively. Results: Of the 77 presumptive PSA isolates, a large proportion (75 to 97.4%) were positively identified. All (100%) of the presumptive 27 ACB harbored the ACB-specific <i>ITS</i> gene fragment by PCR. Twenty five percent of the PSA isolates produced ESBLs phenotypically while more than 90% of these isolates were positive for the <i>lasB, exoA, pslA</i> and <i>exoS</i> genes. A large proportion (88%) of the ACB isolates harboured the <i>OmpA</i> and <i>csuE</i> genes. <i>bla<sub>TEM</sub></i> and <i>bla<sub>CTXM</sub></i> were detected in 17 and 4% of PSA, respectively, while a much higher proportion (70 and 29%) of the ACB isolates possessed these resistance determinants respectively. Conclusion: Our findings reveal the occurrence of both virulence and drug-resistant determinants in clinical PSA and ACB isolates from patients in health care settings in Yaoundé, Cameroon, thus suggesting their role in the pathological conditions in patients. |
| first_indexed | 2024-03-10T19:54:26Z |
| format | Article |
| id | doaj.art-56340285b71049ef828b130f2ec596fb |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T19:54:26Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-56340285b71049ef828b130f2ec596fb2023-11-20T00:03:16ZengMDPI AGMicroorganisms2076-26072020-05-018570810.3390/microorganisms8050708Occurrence of <i>bla<sub>TEM</sub></i> and <i>bla<sub>CTXM</sub></i> Genes and Biofilm-Forming Ability among Clinical Isolates of <i>Pseudomonas aeruginosa</i> and <i>Acinetobacter baumannii</i> in Yaoundé, CameroonEstelle Longla Madaha0Hortense Kamga Gonsu1Rhoda Nsen Bughe2Marie Christine Fonkoua3Collins Njie Ateba4Wilfred Fon Mbacham5Biotechnology Centre, Faculty of Science, University of Yaoundé 1, Yaoundé, CameroonLaboratory of Bacteriology, Yaoundé University Teaching Hospital, Yaoundé, CameroonBiotechnology Centre, Faculty of Science, University of Yaoundé 1, Yaoundé, CameroonBacteriology Service, Centre Pasteur du Cameroun, Yaoundé, CameroonAntibiotic Resistance and Phage Biocontrol Research Group, Department of Microbiology, Faculty of Natural and Agricultural Sciences, North-West University, Mafikeng Campus, Private Bag X2046, Mmabatho 2735, South AfricaBiotechnology Centre, Faculty of Science, University of Yaoundé 1, Yaoundé, CameroonBackground: <i>Pseudomonas aeruginosa</i> (PSA) and <i>Acinetobacter baumannii</i> (ACB) are non-fermentative bacteria mostly associated with nosocomial infections in humans. Objective: This study aimed to determine the antimicrobial resistance profiles and virulence gene of PSA and ACB previously isolated from humans in selected health facilities in Yaoundé, Cameroon. Methods: A total of 77 and 27 presumptive PSA and ACB isolates, respectively, were collected from the Yaoundé teaching hospital. These isolates were previously isolated from various samples including pus, blood and broncho-alveolar lavage. The identities of the isolates were determined through polymerase chain reaction (PCR) amplification of PSA and ACB specific sequences. Antimicrobial susceptibility testing (AST) was performed using the Kirby–Bauer disc diffusion method. Phenotypical expression of AmpC β-lactamases (<i>Amp</i>C), extended spectrum β-lactamases (ESBLs) and metallo β-Lactamases (MBLs) were determined using the combined disc method. Bacterial genomes were screened for the presence of β-lactamases <i>bla<sub>TEM</sub></i> and <i>bla<sub>CTXM</sub></i> genes using specific PCR. The pathogenicity of PSA and ACB was assessed through amplification of the <i>lasB, exoA, pslA</i> and <i>exoS</i> as well as <i>OmpA</i> and <i>csuE</i> virulence genes, respectively. Results: Of the 77 presumptive PSA isolates, a large proportion (75 to 97.4%) were positively identified. All (100%) of the presumptive 27 ACB harbored the ACB-specific <i>ITS</i> gene fragment by PCR. Twenty five percent of the PSA isolates produced ESBLs phenotypically while more than 90% of these isolates were positive for the <i>lasB, exoA, pslA</i> and <i>exoS</i> genes. A large proportion (88%) of the ACB isolates harboured the <i>OmpA</i> and <i>csuE</i> genes. <i>bla<sub>TEM</sub></i> and <i>bla<sub>CTXM</sub></i> were detected in 17 and 4% of PSA, respectively, while a much higher proportion (70 and 29%) of the ACB isolates possessed these resistance determinants respectively. Conclusion: Our findings reveal the occurrence of both virulence and drug-resistant determinants in clinical PSA and ACB isolates from patients in health care settings in Yaoundé, Cameroon, thus suggesting their role in the pathological conditions in patients.https://www.mdpi.com/2076-2607/8/5/708<i>Pseudomonas aeruginosa</i><i>Acinetobacter baumannii</i>antimicrobial susceptibility testingphenotypingPCRvirulence and resistance genes |
| spellingShingle | Estelle Longla Madaha Hortense Kamga Gonsu Rhoda Nsen Bughe Marie Christine Fonkoua Collins Njie Ateba Wilfred Fon Mbacham Occurrence of <i>bla<sub>TEM</sub></i> and <i>bla<sub>CTXM</sub></i> Genes and Biofilm-Forming Ability among Clinical Isolates of <i>Pseudomonas aeruginosa</i> and <i>Acinetobacter baumannii</i> in Yaoundé, Cameroon Microorganisms <i>Pseudomonas aeruginosa</i> <i>Acinetobacter baumannii</i> antimicrobial susceptibility testing phenotyping PCR virulence and resistance genes |
| title | Occurrence of <i>bla<sub>TEM</sub></i> and <i>bla<sub>CTXM</sub></i> Genes and Biofilm-Forming Ability among Clinical Isolates of <i>Pseudomonas aeruginosa</i> and <i>Acinetobacter baumannii</i> in Yaoundé, Cameroon |
| title_full | Occurrence of <i>bla<sub>TEM</sub></i> and <i>bla<sub>CTXM</sub></i> Genes and Biofilm-Forming Ability among Clinical Isolates of <i>Pseudomonas aeruginosa</i> and <i>Acinetobacter baumannii</i> in Yaoundé, Cameroon |
| title_fullStr | Occurrence of <i>bla<sub>TEM</sub></i> and <i>bla<sub>CTXM</sub></i> Genes and Biofilm-Forming Ability among Clinical Isolates of <i>Pseudomonas aeruginosa</i> and <i>Acinetobacter baumannii</i> in Yaoundé, Cameroon |
| title_full_unstemmed | Occurrence of <i>bla<sub>TEM</sub></i> and <i>bla<sub>CTXM</sub></i> Genes and Biofilm-Forming Ability among Clinical Isolates of <i>Pseudomonas aeruginosa</i> and <i>Acinetobacter baumannii</i> in Yaoundé, Cameroon |
| title_short | Occurrence of <i>bla<sub>TEM</sub></i> and <i>bla<sub>CTXM</sub></i> Genes and Biofilm-Forming Ability among Clinical Isolates of <i>Pseudomonas aeruginosa</i> and <i>Acinetobacter baumannii</i> in Yaoundé, Cameroon |
| title_sort | occurrence of i bla sub tem sub i and i bla sub ctxm sub i genes and biofilm forming ability among clinical isolates of i pseudomonas aeruginosa i and i acinetobacter baumannii i in yaounde cameroon |
| topic | <i>Pseudomonas aeruginosa</i> <i>Acinetobacter baumannii</i> antimicrobial susceptibility testing phenotyping PCR virulence and resistance genes |
| url | https://www.mdpi.com/2076-2607/8/5/708 |
| work_keys_str_mv | AT estellelonglamadaha occurrenceofiblasubtemsubiandiblasubctxmsubigenesandbiofilmformingabilityamongclinicalisolatesofipseudomonasaeruginosaiandiacinetobacterbaumanniiiinyaoundecameroon AT hortensekamgagonsu occurrenceofiblasubtemsubiandiblasubctxmsubigenesandbiofilmformingabilityamongclinicalisolatesofipseudomonasaeruginosaiandiacinetobacterbaumanniiiinyaoundecameroon AT rhodansenbughe occurrenceofiblasubtemsubiandiblasubctxmsubigenesandbiofilmformingabilityamongclinicalisolatesofipseudomonasaeruginosaiandiacinetobacterbaumanniiiinyaoundecameroon AT mariechristinefonkoua occurrenceofiblasubtemsubiandiblasubctxmsubigenesandbiofilmformingabilityamongclinicalisolatesofipseudomonasaeruginosaiandiacinetobacterbaumanniiiinyaoundecameroon AT collinsnjieateba occurrenceofiblasubtemsubiandiblasubctxmsubigenesandbiofilmformingabilityamongclinicalisolatesofipseudomonasaeruginosaiandiacinetobacterbaumanniiiinyaoundecameroon AT wilfredfonmbacham occurrenceofiblasubtemsubiandiblasubctxmsubigenesandbiofilmformingabilityamongclinicalisolatesofipseudomonasaeruginosaiandiacinetobacterbaumanniiiinyaoundecameroon |