Genistein Content and Tyrosinase Inhibitory Activity of Edamame (Glycine max) Extracts
The study aimed to determine genistein content and tyrosinase inhibition activity of 70% ethanolic and ethyl acetate extracts of edamame (Glycine max) with an extraction time of 15, 30, and 60 minutes. Extraction was performed using the ultrasonication method. Determination of genistein content was...
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| Format: | Article |
| Language: | English |
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Mulawarman University
2022-11-01
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| Series: | Journal of Tropical Pharmacy and Chemistry |
| Subjects: | |
| Online Access: | https://jtpc.farmasi.unmul.ac.id/index.php/jtpc/article/view/461 |
| _version_ | 1827771080440283136 |
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| author | Indah Yulia Ningsih Lisa Kusuma Wardhani Annisa Ragdha Eka Nuryuanda Endah Puspitasari Mochammad Amrun Hidayat |
| author_facet | Indah Yulia Ningsih Lisa Kusuma Wardhani Annisa Ragdha Eka Nuryuanda Endah Puspitasari Mochammad Amrun Hidayat |
| author_sort | Indah Yulia Ningsih |
| collection | DOAJ |
| description |
The study aimed to determine genistein content and tyrosinase inhibition activity of 70% ethanolic and ethyl acetate extracts of edamame (Glycine max) with an extraction time of 15, 30, and 60 minutes. Extraction was performed using the ultrasonication method. Determination of genistein content was carried out using TLC-densitometry. Furthermore, all samples were tested for their tyrosinase inhibition activity using the spectrophotometric assay. Ethyl acetate extract with 60 min of extraction time exhibited the highest extraction yield (6.414% w/w), the highest genistein content (0.169 ± 0.007% w/w), and the highest tyrosinase inhibition activity (IC50 = 72.420 ± 0.550 µg/mL). It was known that the genistein content of 70% ethanolic and ethyl acetate extracts affected tyrosinase inhibition activity with correlation coefficient (r) values of 0.9973 and 0.9826, respectively. G. max was suggested as a tyrosinase inhibitor agent from natural sources for skin whitening product development due to its isoflavones content, mainly genistein.
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| first_indexed | 2024-03-11T12:49:18Z |
| format | Article |
| id | doaj.art-563723db246e4370a1a7d25b816a7f30 |
| institution | Directory Open Access Journal |
| issn | 2087-7099 2407-6090 |
| language | English |
| last_indexed | 2024-03-11T12:49:18Z |
| publishDate | 2022-11-01 |
| publisher | Mulawarman University |
| record_format | Article |
| series | Journal of Tropical Pharmacy and Chemistry |
| spelling | doaj.art-563723db246e4370a1a7d25b816a7f302023-11-04T05:57:35ZengMulawarman UniversityJournal of Tropical Pharmacy and Chemistry2087-70992407-60902022-11-016210.25026/jtpc.v6i2.461Genistein Content and Tyrosinase Inhibitory Activity of Edamame (Glycine max) ExtractsIndah Yulia NingsihLisa Kusuma WardhaniAnnisa Ragdha Eka NuryuandaEndah PuspitasariMochammad Amrun Hidayat0Faculty of Pharmacy, University of Jember, Indonesia The study aimed to determine genistein content and tyrosinase inhibition activity of 70% ethanolic and ethyl acetate extracts of edamame (Glycine max) with an extraction time of 15, 30, and 60 minutes. Extraction was performed using the ultrasonication method. Determination of genistein content was carried out using TLC-densitometry. Furthermore, all samples were tested for their tyrosinase inhibition activity using the spectrophotometric assay. Ethyl acetate extract with 60 min of extraction time exhibited the highest extraction yield (6.414% w/w), the highest genistein content (0.169 ± 0.007% w/w), and the highest tyrosinase inhibition activity (IC50 = 72.420 ± 0.550 µg/mL). It was known that the genistein content of 70% ethanolic and ethyl acetate extracts affected tyrosinase inhibition activity with correlation coefficient (r) values of 0.9973 and 0.9826, respectively. G. max was suggested as a tyrosinase inhibitor agent from natural sources for skin whitening product development due to its isoflavones content, mainly genistein. https://jtpc.farmasi.unmul.ac.id/index.php/jtpc/article/view/461EdamameGlycine maxgenisteintyrosinase inhibitor |
| spellingShingle | Indah Yulia Ningsih Lisa Kusuma Wardhani Annisa Ragdha Eka Nuryuanda Endah Puspitasari Mochammad Amrun Hidayat Genistein Content and Tyrosinase Inhibitory Activity of Edamame (Glycine max) Extracts Journal of Tropical Pharmacy and Chemistry Edamame Glycine max genistein tyrosinase inhibitor |
| title | Genistein Content and Tyrosinase Inhibitory Activity of Edamame (Glycine max) Extracts |
| title_full | Genistein Content and Tyrosinase Inhibitory Activity of Edamame (Glycine max) Extracts |
| title_fullStr | Genistein Content and Tyrosinase Inhibitory Activity of Edamame (Glycine max) Extracts |
| title_full_unstemmed | Genistein Content and Tyrosinase Inhibitory Activity of Edamame (Glycine max) Extracts |
| title_short | Genistein Content and Tyrosinase Inhibitory Activity of Edamame (Glycine max) Extracts |
| title_sort | genistein content and tyrosinase inhibitory activity of edamame glycine max extracts |
| topic | Edamame Glycine max genistein tyrosinase inhibitor |
| url | https://jtpc.farmasi.unmul.ac.id/index.php/jtpc/article/view/461 |
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