Contrasting Nitrogen Fertilization and Brassica napus (Canola) Variety Development Impact Recruitment of the Root-Associated Microbiome
Canola (Brassica napus) is an important broadacre crop, produced under high nitrogen (N) fertilizer application. Modern canola varieties are developed under high N rates but the impacts on root-associated microbiomes of different varieties are unknown. We studied eight canola varieties spanning hist...
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2023-06-01
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| Series: | Phytobiomes Journal |
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| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/PBIOMES-07-22-0045-R |
| _version_ | 1797804661311275008 |
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| author | Yunliang Li Sally L. Vail Melissa M. Arcand Bobbi L. Helgason |
| author_facet | Yunliang Li Sally L. Vail Melissa M. Arcand Bobbi L. Helgason |
| author_sort | Yunliang Li |
| collection | DOAJ |
| description | Canola (Brassica napus) is an important broadacre crop, produced under high nitrogen (N) fertilizer application. Modern canola varieties are developed under high N rates but the impacts on root-associated microbiomes of different varieties are unknown. We studied eight canola varieties spanning historical Canadian spring canola development at two sites under high and low N fertility and characterized bacterial and fungal microbiomes in the root and rhizosphere using amplicon sequencing. Environmental conditions and the resulting canola varietal responses strongly affected the root-associated bacterial and fungal microbiomes. Microbes regulated by N fertility in each canola variety were mainly Gammaproteobacteria, Bacteroidia, Actinobacteria, Sordariomycetes, Dothideomycetes, and Agaricomycetes classes. Differentially abundant (DA) microbial taxa showed that N more strongly enriched bacteria in the roots and fungi in the rhizosphere. Each variety had its specific pattern of DA amplicon sequence variants (ASVs) responding to soil N availability, and the profile of DA-ASVs in paired canola varieties were also altered by soil N availability, especially bacteria in the rhizosphere. The yield was strongly associated with a subset of microbial taxa, mainly from Proteobacteria, Actinobacteriota, and Ascomycota. These variety-dependent responses to N and links to yield performance make the root-associated microbiome a promising target for improving the agronomic performance of canola by manipulating microorganisms tailored to soil fertility and plant genotype. |
| first_indexed | 2024-03-13T05:40:34Z |
| format | Article |
| id | doaj.art-5637650fa2ca4d0e8262cc9c4ec6fda0 |
| institution | Directory Open Access Journal |
| issn | 2471-2906 |
| language | English |
| last_indexed | 2024-03-13T05:40:34Z |
| publishDate | 2023-06-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Phytobiomes Journal |
| spelling | doaj.art-5637650fa2ca4d0e8262cc9c4ec6fda02023-06-14T01:28:02ZengThe American Phytopathological SocietyPhytobiomes Journal2471-29062023-06-017112513710.1094/PBIOMES-07-22-0045-RContrasting Nitrogen Fertilization and Brassica napus (Canola) Variety Development Impact Recruitment of the Root-Associated MicrobiomeYunliang Li0Sally L. Vail1Melissa M. Arcand2Bobbi L. Helgason3Department of Soil Science, University of Saskatchewan, 51 Campus Drive, Saskatoon SK, S7N 5A8, CanadaSaskatoon Research and Development Centre, Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon SK, S7N 0X2, CanadaDepartment of Soil Science, University of Saskatchewan, 51 Campus Drive, Saskatoon SK, S7N 5A8, CanadaDepartment of Soil Science, University of Saskatchewan, 51 Campus Drive, Saskatoon SK, S7N 5A8, CanadaCanola (Brassica napus) is an important broadacre crop, produced under high nitrogen (N) fertilizer application. Modern canola varieties are developed under high N rates but the impacts on root-associated microbiomes of different varieties are unknown. We studied eight canola varieties spanning historical Canadian spring canola development at two sites under high and low N fertility and characterized bacterial and fungal microbiomes in the root and rhizosphere using amplicon sequencing. Environmental conditions and the resulting canola varietal responses strongly affected the root-associated bacterial and fungal microbiomes. Microbes regulated by N fertility in each canola variety were mainly Gammaproteobacteria, Bacteroidia, Actinobacteria, Sordariomycetes, Dothideomycetes, and Agaricomycetes classes. Differentially abundant (DA) microbial taxa showed that N more strongly enriched bacteria in the roots and fungi in the rhizosphere. Each variety had its specific pattern of DA amplicon sequence variants (ASVs) responding to soil N availability, and the profile of DA-ASVs in paired canola varieties were also altered by soil N availability, especially bacteria in the rhizosphere. The yield was strongly associated with a subset of microbial taxa, mainly from Proteobacteria, Actinobacteriota, and Ascomycota. These variety-dependent responses to N and links to yield performance make the root-associated microbiome a promising target for improving the agronomic performance of canola by manipulating microorganisms tailored to soil fertility and plant genotype.https://apsjournals.apsnet.org/doi/10.1094/PBIOMES-07-22-0045-RBrassica napuscanola varietynitrogen fertilizationroot-associated microbiomeyield |
| spellingShingle | Yunliang Li Sally L. Vail Melissa M. Arcand Bobbi L. Helgason Contrasting Nitrogen Fertilization and Brassica napus (Canola) Variety Development Impact Recruitment of the Root-Associated Microbiome Phytobiomes Journal Brassica napus canola variety nitrogen fertilization root-associated microbiome yield |
| title | Contrasting Nitrogen Fertilization and Brassica napus (Canola) Variety Development Impact Recruitment of the Root-Associated Microbiome |
| title_full | Contrasting Nitrogen Fertilization and Brassica napus (Canola) Variety Development Impact Recruitment of the Root-Associated Microbiome |
| title_fullStr | Contrasting Nitrogen Fertilization and Brassica napus (Canola) Variety Development Impact Recruitment of the Root-Associated Microbiome |
| title_full_unstemmed | Contrasting Nitrogen Fertilization and Brassica napus (Canola) Variety Development Impact Recruitment of the Root-Associated Microbiome |
| title_short | Contrasting Nitrogen Fertilization and Brassica napus (Canola) Variety Development Impact Recruitment of the Root-Associated Microbiome |
| title_sort | contrasting nitrogen fertilization and brassica napus canola variety development impact recruitment of the root associated microbiome |
| topic | Brassica napus canola variety nitrogen fertilization root-associated microbiome yield |
| url | https://apsjournals.apsnet.org/doi/10.1094/PBIOMES-07-22-0045-R |
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