The Applicability of Current Turbidimetric Approaches for Analyzing Fibrin Fibers and Other Filamentous Networks
Turbidimetry is an experimental technique often used to study the structure of filamentous networks. To extract structural properties such as filament diameter from turbidimetric data, simplifications to light scattering theory must be employed. In this work, we evaluate the applicability of three c...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/12/6/807 |
| _version_ | 1797489528503533568 |
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| author | Heather A. Belcher Karen Litwa Martin Guthold Nathan E. Hudson |
| author_facet | Heather A. Belcher Karen Litwa Martin Guthold Nathan E. Hudson |
| author_sort | Heather A. Belcher |
| collection | DOAJ |
| description | Turbidimetry is an experimental technique often used to study the structure of filamentous networks. To extract structural properties such as filament diameter from turbidimetric data, simplifications to light scattering theory must be employed. In this work, we evaluate the applicability of three commonly utilized turbidimetric analysis approaches, each using slightly different simplifications. We make a specific application towards analyzing fibrin fibers, which form the structural scaffold of blood clots, but the results are generalizable. Numerical simulations were utilized to assess the applicability of each approach across a range of fiber lengths and diameters. Simulation results indicated that all three turbidimetric approaches commonly underestimate fiber diameter, and that the “Carr-Hermans” approach, utilizing wavelengths in the range of 500–800 nm, provided <10% error for the largest number of diameter/length combinations. These theoretical results were confirmed, under select conditions, via the comparison of fiber diameters extracted from experimental turbidimetric data, with diameters obtained using super-resolution microscopy. |
| first_indexed | 2024-03-10T00:18:51Z |
| format | Article |
| id | doaj.art-56376e64a0194c679f6256a17d6f5eda |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T00:18:51Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-56376e64a0194c679f6256a17d6f5eda2023-11-23T15:47:16ZengMDPI AGBiomolecules2218-273X2022-06-0112680710.3390/biom12060807The Applicability of Current Turbidimetric Approaches for Analyzing Fibrin Fibers and Other Filamentous NetworksHeather A. Belcher0Karen Litwa1Martin Guthold2Nathan E. Hudson3Department of Physics, East Carolina University, Greenville, NC 27858, USADepartment of Anatomy & Cell Biology, East Carolina University, Greenville, NC 27858, USADepartment of Physics, Wake Forest University, Winston-Salem, NC 27109, USADepartment of Physics, East Carolina University, Greenville, NC 27858, USATurbidimetry is an experimental technique often used to study the structure of filamentous networks. To extract structural properties such as filament diameter from turbidimetric data, simplifications to light scattering theory must be employed. In this work, we evaluate the applicability of three commonly utilized turbidimetric analysis approaches, each using slightly different simplifications. We make a specific application towards analyzing fibrin fibers, which form the structural scaffold of blood clots, but the results are generalizable. Numerical simulations were utilized to assess the applicability of each approach across a range of fiber lengths and diameters. Simulation results indicated that all three turbidimetric approaches commonly underestimate fiber diameter, and that the “Carr-Hermans” approach, utilizing wavelengths in the range of 500–800 nm, provided <10% error for the largest number of diameter/length combinations. These theoretical results were confirmed, under select conditions, via the comparison of fiber diameters extracted from experimental turbidimetric data, with diameters obtained using super-resolution microscopy.https://www.mdpi.com/2218-273X/12/6/807turbidityturbidimetryfibrinfilamentous networkslight scattering |
| spellingShingle | Heather A. Belcher Karen Litwa Martin Guthold Nathan E. Hudson The Applicability of Current Turbidimetric Approaches for Analyzing Fibrin Fibers and Other Filamentous Networks Biomolecules turbidity turbidimetry fibrin filamentous networks light scattering |
| title | The Applicability of Current Turbidimetric Approaches for Analyzing Fibrin Fibers and Other Filamentous Networks |
| title_full | The Applicability of Current Turbidimetric Approaches for Analyzing Fibrin Fibers and Other Filamentous Networks |
| title_fullStr | The Applicability of Current Turbidimetric Approaches for Analyzing Fibrin Fibers and Other Filamentous Networks |
| title_full_unstemmed | The Applicability of Current Turbidimetric Approaches for Analyzing Fibrin Fibers and Other Filamentous Networks |
| title_short | The Applicability of Current Turbidimetric Approaches for Analyzing Fibrin Fibers and Other Filamentous Networks |
| title_sort | applicability of current turbidimetric approaches for analyzing fibrin fibers and other filamentous networks |
| topic | turbidity turbidimetry fibrin filamentous networks light scattering |
| url | https://www.mdpi.com/2218-273X/12/6/807 |
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