A cyclic effect of cAMP and calcium signaling contributes to jujube growth and development
3′,5′-Cyclic adenosine monophosphate (cAMP) is an important metabolite that is specifically enriched in jujube. However, the effect of cAMP on jujube cellular responses has not been comprehensively studied. Here, we established jujube cell suspension cultures and investigated the calcium influx in r...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-07-01
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| Series: | Journal of Integrative Agriculture |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311923001090 |
| _version_ | 1827895777113931776 |
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| author | Li-xin WANG Lin-xia WANG Meng-ling ZHANG Ying-yue QU Ye YUAN Ehsan SADEGHNEZHAD Meng-jiao GAO Ruo-yu ZHAO Chao-feng QI Xiao-xue GUO Wen-hui ZHU Rui-mei LI Li DAI Meng-jun LIU Zhi-guo LIU |
| author_facet | Li-xin WANG Lin-xia WANG Meng-ling ZHANG Ying-yue QU Ye YUAN Ehsan SADEGHNEZHAD Meng-jiao GAO Ruo-yu ZHAO Chao-feng QI Xiao-xue GUO Wen-hui ZHU Rui-mei LI Li DAI Meng-jun LIU Zhi-guo LIU |
| author_sort | Li-xin WANG |
| collection | DOAJ |
| description | 3′,5′-Cyclic adenosine monophosphate (cAMP) is an important metabolite that is specifically enriched in jujube. However, the effect of cAMP on jujube cellular responses has not been comprehensively studied. Here, we established jujube cell suspension cultures and investigated the calcium influx in response to cAMP treatment through protoplast isolation and fluorescence intensity. Firstly, cAMP treatment could promote jujube growth and increase the content of endogenous cAMP. Using transcriptome analysis with transgenic Arabidopsis plants overexpressing adenylate cyclase (ZjAC) as a positive control, we identified 60 calcium-related differential expressed genes (DEGs) that contributed to the calcium signaling and inter- or intra-cellular responses. Pharmacological treatments such as cAMP and the calcium ionophore A23187 could induce ZjAC expression, the accumulation of cAMP and calcium influx in jujube cells, while ethylene glycol tetraacetic acid (EGTA) or bithionol treatment inhibited these changes. Moreover, the calcium channels and transporters in calcium influx, such as the ZjCNGC2 channel and the mitogen activated protein (MAP) kinase pathway, could be activated by cAMP treatment. In summary, our findings demonstrated that cAMP biosynthesis is dependent on calcium influx and the amplifying effect between calcium and cAMP may be involved in intracellular signal induction, which might contribute to the growth and development of jujube. |
| first_indexed | 2024-03-12T22:26:36Z |
| format | Article |
| id | doaj.art-5640445ddd944e6a9f18ebf178f0b55b |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-03-12T22:26:36Z |
| publishDate | 2023-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-5640445ddd944e6a9f18ebf178f0b55b2023-07-22T04:51:48ZengElsevierJournal of Integrative Agriculture2095-31192023-07-0122720942110A cyclic effect of cAMP and calcium signaling contributes to jujube growth and developmentLi-xin WANG0Lin-xia WANG1Meng-ling ZHANG2Ying-yue QU3Ye YUAN4Ehsan SADEGHNEZHAD5Meng-jiao GAO6Ruo-yu ZHAO7Chao-feng QI8Xiao-xue GUO9Wen-hui ZHU10Rui-mei LI11Li DAI12Meng-jun LIU13Zhi-guo LIU14College of Horticulture, Hebei Agricultural University, Baoding 071001, P.R.China; Research Center of Chinese Jujube, Hebei Agricultural University, Baoding 071001, P.R.ChinaCollege of Horticulture, Hebei Agricultural University, Baoding 071001, P.R.China; Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education/College of Life Sciences, Northwest University, Xi’an 710069, P.R.ChinaCollege of Horticulture, Hebei Agricultural University, Baoding 071001, P.R.ChinaCollege of Horticulture, Hebei Agricultural University, Baoding 071001, P.R.ChinaCollege of Horticulture, Hebei Agricultural University, Baoding 071001, P.R.ChinaDepartment of Plant Biology, Faculty of Biological Sciences, Tarbiat Modares University, Teheran 14115, IranCollege of Horticulture, Hebei Agricultural University, Baoding 071001, P.R.ChinaCollege of Horticulture, Hebei Agricultural University, Baoding 071001, P.R.ChinaCollege of Horticulture, Hebei Agricultural University, Baoding 071001, P.R.ChinaCollege of Horticulture, Hebei Agricultural University, Baoding 071001, P.R.ChinaCollege of Horticulture, Hebei Agricultural University, Baoding 071001, P.R.ChinaCollege of Horticulture, Hebei Agricultural University, Baoding 071001, P.R.ChinaCollege of Horticulture, Hebei Agricultural University, Baoding 071001, P.R.China; Research Center of Chinese Jujube, Hebei Agricultural University, Baoding 071001, P.R.ChinaCollege of Horticulture, Hebei Agricultural University, Baoding 071001, P.R.China; Research Center of Chinese Jujube, Hebei Agricultural University, Baoding 071001, P.R.ChinaCollege of Horticulture, Hebei Agricultural University, Baoding 071001, P.R.China; Research Center of Chinese Jujube, Hebei Agricultural University, Baoding 071001, P.R.China; Correspondence LIU Zhi-guo, Tel/Fax: +86-312-75289623′,5′-Cyclic adenosine monophosphate (cAMP) is an important metabolite that is specifically enriched in jujube. However, the effect of cAMP on jujube cellular responses has not been comprehensively studied. Here, we established jujube cell suspension cultures and investigated the calcium influx in response to cAMP treatment through protoplast isolation and fluorescence intensity. Firstly, cAMP treatment could promote jujube growth and increase the content of endogenous cAMP. Using transcriptome analysis with transgenic Arabidopsis plants overexpressing adenylate cyclase (ZjAC) as a positive control, we identified 60 calcium-related differential expressed genes (DEGs) that contributed to the calcium signaling and inter- or intra-cellular responses. Pharmacological treatments such as cAMP and the calcium ionophore A23187 could induce ZjAC expression, the accumulation of cAMP and calcium influx in jujube cells, while ethylene glycol tetraacetic acid (EGTA) or bithionol treatment inhibited these changes. Moreover, the calcium channels and transporters in calcium influx, such as the ZjCNGC2 channel and the mitogen activated protein (MAP) kinase pathway, could be activated by cAMP treatment. In summary, our findings demonstrated that cAMP biosynthesis is dependent on calcium influx and the amplifying effect between calcium and cAMP may be involved in intracellular signal induction, which might contribute to the growth and development of jujube.http://www.sciencedirect.com/science/article/pii/S2095311923001090cAMPcalciumgrowthsuspension cellsprotoplastsignaling |
| spellingShingle | Li-xin WANG Lin-xia WANG Meng-ling ZHANG Ying-yue QU Ye YUAN Ehsan SADEGHNEZHAD Meng-jiao GAO Ruo-yu ZHAO Chao-feng QI Xiao-xue GUO Wen-hui ZHU Rui-mei LI Li DAI Meng-jun LIU Zhi-guo LIU A cyclic effect of cAMP and calcium signaling contributes to jujube growth and development Journal of Integrative Agriculture cAMP calcium growth suspension cells protoplast signaling |
| title | A cyclic effect of cAMP and calcium signaling contributes to jujube growth and development |
| title_full | A cyclic effect of cAMP and calcium signaling contributes to jujube growth and development |
| title_fullStr | A cyclic effect of cAMP and calcium signaling contributes to jujube growth and development |
| title_full_unstemmed | A cyclic effect of cAMP and calcium signaling contributes to jujube growth and development |
| title_short | A cyclic effect of cAMP and calcium signaling contributes to jujube growth and development |
| title_sort | cyclic effect of camp and calcium signaling contributes to jujube growth and development |
| topic | cAMP calcium growth suspension cells protoplast signaling |
| url | http://www.sciencedirect.com/science/article/pii/S2095311923001090 |
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