The effects of alpha-tocopherol supplementation on fracture healing in a postmenopausal osteoporotic rat model
OBJECTIVE: Osteoporosis increases the risk of bone fractures and may impair fracture healing. The aim of this study was to investigate whether alpha-tocopherol can improve the late-phase fracture healing of osteoporotic bones in ovariectomized rats. METHOD: In total, 24 female Sprague-Dawley rats we...
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| Format: | Article |
| Language: | English |
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Elsevier España
2012-09-01
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| Series: | Clinics |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322012000900016 |
| _version_ | 1811230184445050880 |
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| author | Sharlina Mohamad Ahmad Nazrun Shuid Norazlina Mohamed Fazalina Mohd Fadzilah Sabarul Afian Mokhtar Shahrum Abdullah Faizah Othman Farihah Suhaimi Norliza Muhammad Ima Nirwana Soelaiman |
| author_facet | Sharlina Mohamad Ahmad Nazrun Shuid Norazlina Mohamed Fazalina Mohd Fadzilah Sabarul Afian Mokhtar Shahrum Abdullah Faizah Othman Farihah Suhaimi Norliza Muhammad Ima Nirwana Soelaiman |
| author_sort | Sharlina Mohamad |
| collection | DOAJ |
| description | OBJECTIVE: Osteoporosis increases the risk of bone fractures and may impair fracture healing. The aim of this study was to investigate whether alpha-tocopherol can improve the late-phase fracture healing of osteoporotic bones in ovariectomized rats. METHOD: In total, 24 female Sprague-Dawley rats were divided into three groups. The first group was sham-operated, and the other two groups were ovariectomized. After two months, the right femora of the rats were fractured under anesthesia and internally repaired with K-wires. The sham-operated and ovariectomized control rat groups were administered olive oil (a vehicle), whereas 60 mg/kg of alpha-tocopherol was administered via oral gavage to the alpha-tocopherol group for six days per week over the course of 8 weeks. The rats were sacrificed, and the femora were dissected out. Computed tomography scans and X-rays were performed to assess fracture healing and callus staging, followed by the assessment of callus strengths through the biomechanical testing of the bones. RESULTS: Significantly higher callus volume and callus staging were observed in the ovariectomized control group compared with the sham-operated and alpha-tocopherol groups. The ovariectomized control group also had significantly lower fracture healing scores than the sham-operated group. There were no differences between the alpha-tocopherol and sham-operated groups with respect to the above parameters. The healed femora of the ovariectomized control group demonstrated significantly lower load and strain parameters than the healed femora of the sham-operated group. Alpha-tocopherol supplementation was not able to restore these biomechanical properties. CONCLUSION: Alpha-tocopherol supplementation appeared to promote bone fracture healing in osteoporotic rats but failed to restore the strength of the fractured bone. |
| first_indexed | 2024-04-12T10:26:01Z |
| format | Article |
| id | doaj.art-56416d97867841b184dd01bd8e68903c |
| institution | Directory Open Access Journal |
| issn | 1807-5932 1980-5322 |
| language | English |
| last_indexed | 2024-04-12T10:26:01Z |
| publishDate | 2012-09-01 |
| publisher | Elsevier España |
| record_format | Article |
| series | Clinics |
| spelling | doaj.art-56416d97867841b184dd01bd8e68903c2022-12-22T03:36:58ZengElsevier EspañaClinics1807-59321980-53222012-09-016791077108510.6061/clinics/2012(09)16The effects of alpha-tocopherol supplementation on fracture healing in a postmenopausal osteoporotic rat modelSharlina MohamadAhmad Nazrun ShuidNorazlina MohamedFazalina Mohd FadzilahSabarul Afian MokhtarShahrum AbdullahFaizah OthmanFarihah SuhaimiNorliza MuhammadIma Nirwana SoelaimanOBJECTIVE: Osteoporosis increases the risk of bone fractures and may impair fracture healing. The aim of this study was to investigate whether alpha-tocopherol can improve the late-phase fracture healing of osteoporotic bones in ovariectomized rats. METHOD: In total, 24 female Sprague-Dawley rats were divided into three groups. The first group was sham-operated, and the other two groups were ovariectomized. After two months, the right femora of the rats were fractured under anesthesia and internally repaired with K-wires. The sham-operated and ovariectomized control rat groups were administered olive oil (a vehicle), whereas 60 mg/kg of alpha-tocopherol was administered via oral gavage to the alpha-tocopherol group for six days per week over the course of 8 weeks. The rats were sacrificed, and the femora were dissected out. Computed tomography scans and X-rays were performed to assess fracture healing and callus staging, followed by the assessment of callus strengths through the biomechanical testing of the bones. RESULTS: Significantly higher callus volume and callus staging were observed in the ovariectomized control group compared with the sham-operated and alpha-tocopherol groups. The ovariectomized control group also had significantly lower fracture healing scores than the sham-operated group. There were no differences between the alpha-tocopherol and sham-operated groups with respect to the above parameters. The healed femora of the ovariectomized control group demonstrated significantly lower load and strain parameters than the healed femora of the sham-operated group. Alpha-tocopherol supplementation was not able to restore these biomechanical properties. CONCLUSION: Alpha-tocopherol supplementation appeared to promote bone fracture healing in osteoporotic rats but failed to restore the strength of the fractured bone.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322012000900016BoneFractureOsteoporosisVitamin EAlpha-tocopherol |
| spellingShingle | Sharlina Mohamad Ahmad Nazrun Shuid Norazlina Mohamed Fazalina Mohd Fadzilah Sabarul Afian Mokhtar Shahrum Abdullah Faizah Othman Farihah Suhaimi Norliza Muhammad Ima Nirwana Soelaiman The effects of alpha-tocopherol supplementation on fracture healing in a postmenopausal osteoporotic rat model Clinics Bone Fracture Osteoporosis Vitamin E Alpha-tocopherol |
| title | The effects of alpha-tocopherol supplementation on fracture healing in a postmenopausal osteoporotic rat model |
| title_full | The effects of alpha-tocopherol supplementation on fracture healing in a postmenopausal osteoporotic rat model |
| title_fullStr | The effects of alpha-tocopherol supplementation on fracture healing in a postmenopausal osteoporotic rat model |
| title_full_unstemmed | The effects of alpha-tocopherol supplementation on fracture healing in a postmenopausal osteoporotic rat model |
| title_short | The effects of alpha-tocopherol supplementation on fracture healing in a postmenopausal osteoporotic rat model |
| title_sort | effects of alpha tocopherol supplementation on fracture healing in a postmenopausal osteoporotic rat model |
| topic | Bone Fracture Osteoporosis Vitamin E Alpha-tocopherol |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322012000900016 |
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