Antiviral Potential of Sea Urchin Aminated Spinochromes against Herpes Simplex Virus Type 1
Herpes simplex virus type 1 (HSV-1) is one of the most prevalent pathogens worldwide requiring the search for new candidates for the creation of antiherpetic drugs. The ability of sea urchin spinochromes—echinochrome A (EchA) and its aminated analogues, echinamines A (EamA) and B (EamB)—to inhibit d...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-11-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/18/11/550 |
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| author | Natalia P. Mishchenko Natalia V. Krylova Olga V. Iunikhina Elena A. Vasileva Galina N. Likhatskaya Evgeny A. Pislyagin Darya V. Tarbeeva Pavel S. Dmitrenok Sergey A. Fedoreyev |
| author_facet | Natalia P. Mishchenko Natalia V. Krylova Olga V. Iunikhina Elena A. Vasileva Galina N. Likhatskaya Evgeny A. Pislyagin Darya V. Tarbeeva Pavel S. Dmitrenok Sergey A. Fedoreyev |
| author_sort | Natalia P. Mishchenko |
| collection | DOAJ |
| description | Herpes simplex virus type 1 (HSV-1) is one of the most prevalent pathogens worldwide requiring the search for new candidates for the creation of antiherpetic drugs. The ability of sea urchin spinochromes—echinochrome A (EchA) and its aminated analogues, echinamines A (EamA) and B (EamB)—to inhibit different stages of HSV-1 infection in Vero cells and to reduce the virus-induced production of reactive oxygen species (ROS) was studied. We found that spinochromes exhibited maximum antiviral activity when HSV-1 was pretreated with these compounds, which indicated the direct effect of spinochromes on HSV-1 particles. EamB and EamA both showed the highest virucidal activity by inhibiting the HSV-1 plaque formation, with a selectivity index (SI) of 80.6 and 50.3, respectively, and a reduction in HSV-1 attachment to cells (SI of 8.5 and 5.8, respectively). EamA and EamB considerably suppressed the early induction of ROS due to the virus infection. The ability of the tested compounds to directly bind to the surface glycoprotein, gD, of HSV-1 was established in silico. The dock score of EchA, EamA, and EamB was −4.75, −5.09, and −5.19 kcal/mol, respectively, which correlated with the SI of the virucidal action of these compounds and explained their ability to suppress the attachment and penetration of the virus into the cells. |
| first_indexed | 2024-03-10T15:03:57Z |
| format | Article |
| id | doaj.art-56421d1241a44244a941e8ddda7ec333 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-10T15:03:57Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-56421d1241a44244a941e8ddda7ec3332023-11-20T19:54:47ZengMDPI AGMarine Drugs1660-33972020-11-01181155010.3390/md18110550Antiviral Potential of Sea Urchin Aminated Spinochromes against Herpes Simplex Virus Type 1Natalia P. Mishchenko0Natalia V. Krylova1Olga V. Iunikhina2Elena A. Vasileva3Galina N. Likhatskaya4Evgeny A. Pislyagin5Darya V. Tarbeeva6Pavel S. Dmitrenok7Sergey A. Fedoreyev8G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, RussiaG.P. Somov Institute of Epidemiology and Microbiology, Far-Eastern Branch of the Russian Academy of Sciences, 690087 Vladivostok, RussiaG.P. Somov Institute of Epidemiology and Microbiology, Far-Eastern Branch of the Russian Academy of Sciences, 690087 Vladivostok, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, RussiaHerpes simplex virus type 1 (HSV-1) is one of the most prevalent pathogens worldwide requiring the search for new candidates for the creation of antiherpetic drugs. The ability of sea urchin spinochromes—echinochrome A (EchA) and its aminated analogues, echinamines A (EamA) and B (EamB)—to inhibit different stages of HSV-1 infection in Vero cells and to reduce the virus-induced production of reactive oxygen species (ROS) was studied. We found that spinochromes exhibited maximum antiviral activity when HSV-1 was pretreated with these compounds, which indicated the direct effect of spinochromes on HSV-1 particles. EamB and EamA both showed the highest virucidal activity by inhibiting the HSV-1 plaque formation, with a selectivity index (SI) of 80.6 and 50.3, respectively, and a reduction in HSV-1 attachment to cells (SI of 8.5 and 5.8, respectively). EamA and EamB considerably suppressed the early induction of ROS due to the virus infection. The ability of the tested compounds to directly bind to the surface glycoprotein, gD, of HSV-1 was established in silico. The dock score of EchA, EamA, and EamB was −4.75, −5.09, and −5.19 kcal/mol, respectively, which correlated with the SI of the virucidal action of these compounds and explained their ability to suppress the attachment and penetration of the virus into the cells.https://www.mdpi.com/1660-3397/18/11/550echinochrome Aechinamine Aechinamine Bherpes simplex virus type 1Vero cellsglycoprotein gD |
| spellingShingle | Natalia P. Mishchenko Natalia V. Krylova Olga V. Iunikhina Elena A. Vasileva Galina N. Likhatskaya Evgeny A. Pislyagin Darya V. Tarbeeva Pavel S. Dmitrenok Sergey A. Fedoreyev Antiviral Potential of Sea Urchin Aminated Spinochromes against Herpes Simplex Virus Type 1 Marine Drugs echinochrome A echinamine A echinamine B herpes simplex virus type 1 Vero cells glycoprotein gD |
| title | Antiviral Potential of Sea Urchin Aminated Spinochromes against Herpes Simplex Virus Type 1 |
| title_full | Antiviral Potential of Sea Urchin Aminated Spinochromes against Herpes Simplex Virus Type 1 |
| title_fullStr | Antiviral Potential of Sea Urchin Aminated Spinochromes against Herpes Simplex Virus Type 1 |
| title_full_unstemmed | Antiviral Potential of Sea Urchin Aminated Spinochromes against Herpes Simplex Virus Type 1 |
| title_short | Antiviral Potential of Sea Urchin Aminated Spinochromes against Herpes Simplex Virus Type 1 |
| title_sort | antiviral potential of sea urchin aminated spinochromes against herpes simplex virus type 1 |
| topic | echinochrome A echinamine A echinamine B herpes simplex virus type 1 Vero cells glycoprotein gD |
| url | https://www.mdpi.com/1660-3397/18/11/550 |
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