Comparative cytogenetic analysis of two grasshopper species of the tribe Abracrini (Ommatolampinae, Acrididae)
The grasshopper species Orthoscapheus rufipes and Eujivarus fusiformis were analyzed using several cytogenetic techniques. The karyotype of O. rufipes, described here for the first time, had a diploid number of 2n = 23, whereas E. fusiformis had a karyotype with 2n = 21. The two species showed the s...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sociedade Brasileira de Genética
2011-01-01
|
| Series: | Genetics and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200008 |
| _version_ | 1811280042792058880 |
|---|---|
| author | Marília de França Rocha Natoniel Franklin de Melo Maria José de Souza |
| author_facet | Marília de França Rocha Natoniel Franklin de Melo Maria José de Souza |
| author_sort | Marília de França Rocha |
| collection | DOAJ |
| description | The grasshopper species Orthoscapheus rufipes and Eujivarus fusiformis were analyzed using several cytogenetic techniques. The karyotype of O. rufipes, described here for the first time, had a diploid number of 2n = 23, whereas E. fusiformis had a karyotype with 2n = 21. The two species showed the same mechanism of sex determination (XO type) but differed in chromosome morphology. Pericentromeric blocks of constitutive heterochromatin (CH) were detected in the chromosome complement of both species. CMA3/DA/DAPI staining revealed CMA3-positive blocks in CH regions in four autosomal bivalents of O. rufipes and in two of E. fusiformis. The location of active NORs differed between the two species, occurring in bivalents M6 and S9 of O. rufipes and M6 and M7 of E. fusiformsi. The rDNA sites revealed by FISH coincided with the number and position of the active NORs detected by AgNO3 staining. The variability in chromosomal markers accounted for the karyotype differentiation observed in the tribe Abracrini. |
| first_indexed | 2024-04-13T01:06:30Z |
| format | Article |
| id | doaj.art-56483a6102a9487d8223e56b666fadaa |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-04-13T01:06:30Z |
| publishDate | 2011-01-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-56483a6102a9487d8223e56b666fadaa2022-12-22T03:09:18ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852011-01-0134221421910.1590/S1415-47572011000200008Comparative cytogenetic analysis of two grasshopper species of the tribe Abracrini (Ommatolampinae, Acrididae)Marília de França RochaNatoniel Franklin de MeloMaria José de SouzaThe grasshopper species Orthoscapheus rufipes and Eujivarus fusiformis were analyzed using several cytogenetic techniques. The karyotype of O. rufipes, described here for the first time, had a diploid number of 2n = 23, whereas E. fusiformis had a karyotype with 2n = 21. The two species showed the same mechanism of sex determination (XO type) but differed in chromosome morphology. Pericentromeric blocks of constitutive heterochromatin (CH) were detected in the chromosome complement of both species. CMA3/DA/DAPI staining revealed CMA3-positive blocks in CH regions in four autosomal bivalents of O. rufipes and in two of E. fusiformis. The location of active NORs differed between the two species, occurring in bivalents M6 and S9 of O. rufipes and M6 and M7 of E. fusiformsi. The rDNA sites revealed by FISH coincided with the number and position of the active NORs detected by AgNO3 staining. The variability in chromosomal markers accounted for the karyotype differentiation observed in the tribe Abracrini.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200008constitutive heterochromatinfluorochromesFISHOrthopteraribosomal DNA |
| spellingShingle | Marília de França Rocha Natoniel Franklin de Melo Maria José de Souza Comparative cytogenetic analysis of two grasshopper species of the tribe Abracrini (Ommatolampinae, Acrididae) Genetics and Molecular Biology constitutive heterochromatin fluorochromes FISH Orthoptera ribosomal DNA |
| title | Comparative cytogenetic analysis of two grasshopper species of the tribe Abracrini (Ommatolampinae, Acrididae) |
| title_full | Comparative cytogenetic analysis of two grasshopper species of the tribe Abracrini (Ommatolampinae, Acrididae) |
| title_fullStr | Comparative cytogenetic analysis of two grasshopper species of the tribe Abracrini (Ommatolampinae, Acrididae) |
| title_full_unstemmed | Comparative cytogenetic analysis of two grasshopper species of the tribe Abracrini (Ommatolampinae, Acrididae) |
| title_short | Comparative cytogenetic analysis of two grasshopper species of the tribe Abracrini (Ommatolampinae, Acrididae) |
| title_sort | comparative cytogenetic analysis of two grasshopper species of the tribe abracrini ommatolampinae acrididae |
| topic | constitutive heterochromatin fluorochromes FISH Orthoptera ribosomal DNA |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200008 |
| work_keys_str_mv | AT mariliadefrancarocha comparativecytogeneticanalysisoftwograsshopperspeciesofthetribeabracriniommatolampinaeacrididae AT natonielfranklindemelo comparativecytogeneticanalysisoftwograsshopperspeciesofthetribeabracriniommatolampinaeacrididae AT mariajosedesouza comparativecytogeneticanalysisoftwograsshopperspeciesofthetribeabracriniommatolampinaeacrididae |