Complete separation of lipid classes on a single thin-layer plate
A double-development procedure employing first a polar and then a nonpolar solvent system is described for the complete separation by thin-layer chromatography of the main lipid classes encountered in natural lipids. For better quantification, long plates (34 cm) are employed. Diglycerides were sepa...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1966-03-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S002222752039297X |
| _version_ | 1818931493217501184 |
|---|---|
| author | C.P. Freeman D. West |
| author_facet | C.P. Freeman D. West |
| author_sort | C.P. Freeman |
| collection | DOAJ |
| description | A double-development procedure employing first a polar and then a nonpolar solvent system is described for the complete separation by thin-layer chromatography of the main lipid classes encountered in natural lipids. For better quantification, long plates (34 cm) are employed. Diglycerides were separated from cholesterol, 1,2- from 1,3-diglycerides, and monoglycerides from phospholipids. |
| first_indexed | 2024-12-20T04:17:28Z |
| format | Article |
| id | doaj.art-56489ecc783d48d7b7aeff1b5e5f97b6 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-20T04:17:28Z |
| publishDate | 1966-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-56489ecc783d48d7b7aeff1b5e5f97b62022-12-21T19:53:44ZengElsevierJournal of Lipid Research0022-22751966-03-0172324327Complete separation of lipid classes on a single thin-layer plateC.P. Freeman0D. West1Unilever Research Laboratory, Colworth House, Sharnbrook, Bedford, EnglandUnilever Research Laboratory, Colworth House, Sharnbrook, Bedford, EnglandA double-development procedure employing first a polar and then a nonpolar solvent system is described for the complete separation by thin-layer chromatography of the main lipid classes encountered in natural lipids. For better quantification, long plates (34 cm) are employed. Diglycerides were separated from cholesterol, 1,2- from 1,3-diglycerides, and monoglycerides from phospholipids.http://www.sciencedirect.com/science/article/pii/S002222752039297Xdouble-developmentthin-layer chromatographyquantificationlipid classes |
| spellingShingle | C.P. Freeman D. West Complete separation of lipid classes on a single thin-layer plate Journal of Lipid Research double-development thin-layer chromatography quantification lipid classes |
| title | Complete separation of lipid classes on a single thin-layer plate |
| title_full | Complete separation of lipid classes on a single thin-layer plate |
| title_fullStr | Complete separation of lipid classes on a single thin-layer plate |
| title_full_unstemmed | Complete separation of lipid classes on a single thin-layer plate |
| title_short | Complete separation of lipid classes on a single thin-layer plate |
| title_sort | complete separation of lipid classes on a single thin layer plate |
| topic | double-development thin-layer chromatography quantification lipid classes |
| url | http://www.sciencedirect.com/science/article/pii/S002222752039297X |
| work_keys_str_mv | AT cpfreeman completeseparationoflipidclassesonasinglethinlayerplate AT dwest completeseparationoflipidclassesonasinglethinlayerplate |