The Agrobacterium tumefaciens Ti Plasmid Virulence Gene virE2 Reduces Sri Lankan Cassava Mosaic Virus Infection in Transgenic Nicotiana benthamiana Plants
Cassava mosaic disease is a major constraint to cassava cultivation worldwide. In India, the disease is caused by Indian cassava mosaic virus (ICMV) and Sri Lankan cassava mosaic virus (SLCMV). The Agrobacterium Ti plasmid virulence gene virE2, encoding a nuclear-localized, single-stranded DNA bindi...
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MDPI AG
2015-05-01
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| Series: | Viruses |
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| Online Access: | http://www.mdpi.com/1999-4915/7/5/2641 |
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| author | Thulasi Raveendrannair Resmi Thomas Hohn Barbara Hohn Karuppannan Veluthambi |
| author_facet | Thulasi Raveendrannair Resmi Thomas Hohn Barbara Hohn Karuppannan Veluthambi |
| author_sort | Thulasi Raveendrannair Resmi |
| collection | DOAJ |
| description | Cassava mosaic disease is a major constraint to cassava cultivation worldwide. In India, the disease is caused by Indian cassava mosaic virus (ICMV) and Sri Lankan cassava mosaic virus (SLCMV). The Agrobacterium Ti plasmid virulence gene virE2, encoding a nuclear-localized, single-stranded DNA binding protein, was introduced into Nicotiana benthamiana to develop tolerance against SLCMV. Leaf discs of transgenic N. benthamiana plants, harboring the virE2 gene, complemented a virE2 mutation in A. tumefaciens and produced tumours. Three tested virE2 transgenic plants displayed reduction in disease symptoms upon agroinoculation with SLCMV DNA A and DNA B partial dimers. A pronounced reduction in viral DNA accumulation was observed in all three virE2 transgenic plants. Thus, virE2 is an effective candidate gene to develop tolerance against the cassava mosaic disease and possibly other DNA virus diseases. |
| first_indexed | 2024-12-10T11:49:27Z |
| format | Article |
| id | doaj.art-564c2c98aecc42fe9183c516440e1e4d |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-10T11:49:27Z |
| publishDate | 2015-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-564c2c98aecc42fe9183c516440e1e4d2022-12-22T01:49:58ZengMDPI AGViruses1999-49152015-05-01752641265310.3390/v7052641v7052641The Agrobacterium tumefaciens Ti Plasmid Virulence Gene virE2 Reduces Sri Lankan Cassava Mosaic Virus Infection in Transgenic Nicotiana benthamiana PlantsThulasi Raveendrannair Resmi0Thomas Hohn1Barbara Hohn2Karuppannan Veluthambi3Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai, Tamil Nadu 625021, IndiaInstitute of Botany, University of Basel, Schoenbeinstrasse 6, 4056 Basel, SwitzerlandFriedrich Miescher Institute for Biomedical Research, 4058 Basel, SwitzerlandDepartment of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai, Tamil Nadu 625021, IndiaCassava mosaic disease is a major constraint to cassava cultivation worldwide. In India, the disease is caused by Indian cassava mosaic virus (ICMV) and Sri Lankan cassava mosaic virus (SLCMV). The Agrobacterium Ti plasmid virulence gene virE2, encoding a nuclear-localized, single-stranded DNA binding protein, was introduced into Nicotiana benthamiana to develop tolerance against SLCMV. Leaf discs of transgenic N. benthamiana plants, harboring the virE2 gene, complemented a virE2 mutation in A. tumefaciens and produced tumours. Three tested virE2 transgenic plants displayed reduction in disease symptoms upon agroinoculation with SLCMV DNA A and DNA B partial dimers. A pronounced reduction in viral DNA accumulation was observed in all three virE2 transgenic plants. Thus, virE2 is an effective candidate gene to develop tolerance against the cassava mosaic disease and possibly other DNA virus diseases.http://www.mdpi.com/1999-4915/7/5/2641geminivirusagroinfectionssDNA bindingcassava mosaic virusSLCMV |
| spellingShingle | Thulasi Raveendrannair Resmi Thomas Hohn Barbara Hohn Karuppannan Veluthambi The Agrobacterium tumefaciens Ti Plasmid Virulence Gene virE2 Reduces Sri Lankan Cassava Mosaic Virus Infection in Transgenic Nicotiana benthamiana Plants Viruses geminivirus agroinfection ssDNA binding cassava mosaic virus SLCMV |
| title | The Agrobacterium tumefaciens Ti Plasmid Virulence Gene virE2 Reduces Sri Lankan Cassava Mosaic Virus Infection in Transgenic Nicotiana benthamiana Plants |
| title_full | The Agrobacterium tumefaciens Ti Plasmid Virulence Gene virE2 Reduces Sri Lankan Cassava Mosaic Virus Infection in Transgenic Nicotiana benthamiana Plants |
| title_fullStr | The Agrobacterium tumefaciens Ti Plasmid Virulence Gene virE2 Reduces Sri Lankan Cassava Mosaic Virus Infection in Transgenic Nicotiana benthamiana Plants |
| title_full_unstemmed | The Agrobacterium tumefaciens Ti Plasmid Virulence Gene virE2 Reduces Sri Lankan Cassava Mosaic Virus Infection in Transgenic Nicotiana benthamiana Plants |
| title_short | The Agrobacterium tumefaciens Ti Plasmid Virulence Gene virE2 Reduces Sri Lankan Cassava Mosaic Virus Infection in Transgenic Nicotiana benthamiana Plants |
| title_sort | agrobacterium tumefaciens ti plasmid virulence gene vire2 reduces sri lankan cassava mosaic virus infection in transgenic nicotiana benthamiana plants |
| topic | geminivirus agroinfection ssDNA binding cassava mosaic virus SLCMV |
| url | http://www.mdpi.com/1999-4915/7/5/2641 |
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