Characterization and Antimicrobial Susceptibility Pattern of Non-fermenting Gram-negative Bacilli and Molecular Analysis of Pseudomonas Species from Respiratory Samples of Intensive Care Unit Patients in A Tertiary Care Hospital
Among patients admitted to the intensive care unit (ICU) the most common cause of infection is lower respiratory tract infections, which account for 4.4% of hospital admissions. Among the non-fermenters, Pseudomonas aeruginosa and Acinetobacter calcoaceticus-baumannii complex, cause many types of no...
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| Format: | Article |
| Language: | English |
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Journal of Pure and Applied Microbiology
2023-12-01
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| Series: | Journal of Pure and Applied Microbiology |
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| Online Access: | https://microbiologyjournal.org/characterization-and-antimicrobial-susceptibility-pattern-of-non-fermenting-gram-negative-bacilli-and-molecular-analysis-of-pseudomonas-species-from-respiratory-samples-of-intensive-care-unit-patients/ |
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| author | Umamakeshwari Dhanalakshmi Jeyaraman Padmakumari Jagadeesan Vinotha Sundaram Vanaja Rebba |
| author_facet | Umamakeshwari Dhanalakshmi Jeyaraman Padmakumari Jagadeesan Vinotha Sundaram Vanaja Rebba |
| author_sort | Umamakeshwari Dhanalakshmi Jeyaraman |
| collection | DOAJ |
| description | Among patients admitted to the intensive care unit (ICU) the most common cause of infection is lower respiratory tract infections, which account for 4.4% of hospital admissions. Among the non-fermenters, Pseudomonas aeruginosa and Acinetobacter calcoaceticus-baumannii complex, cause many types of nosocomial infections. Ventilator-associated pneumonia is the most common emerging infection among them. The goal of this study was to isolate and speciate non-fermenting Gram-negative organisms from respiratory samples of ICU patients and to detect antimicrobial susceptibility patterns of the isolated non-fermenters. This cross-sectional study was conducted at the Institute of Microbiology in collaboration with Medical Intensive Care units in Rajiv Gandhi Government General Hospital. A total of 200 patients who satisfied the inclusion criteria were included in the study conducted between March 2019 and March 2020. Culture, sensitivity, and anti-microbial susceptibility tests were performed for the respiratory samples collected as per standard protocols. Pseudomonas aeruginosa (49%) was the most commonly isolated non-fermenter followed by Acinetobacter baumannii (24.3%). Among Pseudomonas aeruginosa isolates, the highest percentage were ESBL producers (44.4%). Carbapenem resistance among Pseudomonas isolates was 33%. The study showed increased isolation of MDR non-fermenters from the ICU causing Ventilator-associated pneumonia (VAP). To prevent VAP caused by these MDR pathogens, clinicians should follow strict infection control practices, use invasive devices on a short-term basis, and use antibiotics judiciously. |
| first_indexed | 2024-03-08T22:52:28Z |
| format | Article |
| id | doaj.art-564d7e474c7944e2924287f6574db5dd |
| institution | Directory Open Access Journal |
| issn | 0973-7510 2581-690X |
| language | English |
| last_indexed | 2024-03-08T22:52:28Z |
| publishDate | 2023-12-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj.art-564d7e474c7944e2924287f6574db5dd2023-12-16T10:25:57ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2023-12-0117421792187https://doi.org/10.22207/JPAM.17.4.14Characterization and Antimicrobial Susceptibility Pattern of Non-fermenting Gram-negative Bacilli and Molecular Analysis of Pseudomonas Species from Respiratory Samples of Intensive Care Unit Patients in A Tertiary Care HospitalUmamakeshwari Dhanalakshmi Jeyaramanhttps://orcid.org/0009-0003-8669-9122Padmakumari Jagadeesanhttps://orcid.org/0009-0002-5697-9011Vinotha Sundaramhttps://orcid.org/0000-0003-1625-8869Vanaja Rebbahttps://orcid.org/0009-0001-2465-3917Among patients admitted to the intensive care unit (ICU) the most common cause of infection is lower respiratory tract infections, which account for 4.4% of hospital admissions. Among the non-fermenters, Pseudomonas aeruginosa and Acinetobacter calcoaceticus-baumannii complex, cause many types of nosocomial infections. Ventilator-associated pneumonia is the most common emerging infection among them. The goal of this study was to isolate and speciate non-fermenting Gram-negative organisms from respiratory samples of ICU patients and to detect antimicrobial susceptibility patterns of the isolated non-fermenters. This cross-sectional study was conducted at the Institute of Microbiology in collaboration with Medical Intensive Care units in Rajiv Gandhi Government General Hospital. A total of 200 patients who satisfied the inclusion criteria were included in the study conducted between March 2019 and March 2020. Culture, sensitivity, and anti-microbial susceptibility tests were performed for the respiratory samples collected as per standard protocols. Pseudomonas aeruginosa (49%) was the most commonly isolated non-fermenter followed by Acinetobacter baumannii (24.3%). Among Pseudomonas aeruginosa isolates, the highest percentage were ESBL producers (44.4%). Carbapenem resistance among Pseudomonas isolates was 33%. The study showed increased isolation of MDR non-fermenters from the ICU causing Ventilator-associated pneumonia (VAP). To prevent VAP caused by these MDR pathogens, clinicians should follow strict infection control practices, use invasive devices on a short-term basis, and use antibiotics judiciously.https://microbiologyjournal.org/characterization-and-antimicrobial-susceptibility-pattern-of-non-fermenting-gram-negative-bacilli-and-molecular-analysis-of-pseudomonas-species-from-respiratory-samples-of-intensive-care-unit-patients/non-fermentersventilator associated pneumonia(vap)pseudomonas aeruginosaacinetobacter baumannii |
| spellingShingle | Umamakeshwari Dhanalakshmi Jeyaraman Padmakumari Jagadeesan Vinotha Sundaram Vanaja Rebba Characterization and Antimicrobial Susceptibility Pattern of Non-fermenting Gram-negative Bacilli and Molecular Analysis of Pseudomonas Species from Respiratory Samples of Intensive Care Unit Patients in A Tertiary Care Hospital Journal of Pure and Applied Microbiology non-fermenters ventilator associated pneumonia(vap) pseudomonas aeruginosa acinetobacter baumannii |
| title | Characterization and Antimicrobial Susceptibility Pattern of Non-fermenting Gram-negative Bacilli and Molecular Analysis of Pseudomonas Species from Respiratory Samples of Intensive Care Unit Patients in A Tertiary Care Hospital |
| title_full | Characterization and Antimicrobial Susceptibility Pattern of Non-fermenting Gram-negative Bacilli and Molecular Analysis of Pseudomonas Species from Respiratory Samples of Intensive Care Unit Patients in A Tertiary Care Hospital |
| title_fullStr | Characterization and Antimicrobial Susceptibility Pattern of Non-fermenting Gram-negative Bacilli and Molecular Analysis of Pseudomonas Species from Respiratory Samples of Intensive Care Unit Patients in A Tertiary Care Hospital |
| title_full_unstemmed | Characterization and Antimicrobial Susceptibility Pattern of Non-fermenting Gram-negative Bacilli and Molecular Analysis of Pseudomonas Species from Respiratory Samples of Intensive Care Unit Patients in A Tertiary Care Hospital |
| title_short | Characterization and Antimicrobial Susceptibility Pattern of Non-fermenting Gram-negative Bacilli and Molecular Analysis of Pseudomonas Species from Respiratory Samples of Intensive Care Unit Patients in A Tertiary Care Hospital |
| title_sort | characterization and antimicrobial susceptibility pattern of non fermenting gram negative bacilli and molecular analysis of pseudomonas species from respiratory samples of intensive care unit patients in a tertiary care hospital |
| topic | non-fermenters ventilator associated pneumonia(vap) pseudomonas aeruginosa acinetobacter baumannii |
| url | https://microbiologyjournal.org/characterization-and-antimicrobial-susceptibility-pattern-of-non-fermenting-gram-negative-bacilli-and-molecular-analysis-of-pseudomonas-species-from-respiratory-samples-of-intensive-care-unit-patients/ |
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