Purine Metabolism and Hexosamine Biosynthetic Pathway Abnormalities in Diarrheal Weaned Piglets Identified Using Metabolomics
Post-weaning diarrhea significantly contributes to the high mortality in pig production, but the metabolic changes in weaned piglets with diarrhea remain unclear. This study aimed to identify the differential metabolites in the urine of diarrheal weaned piglets and those of healthy weaned piglets to...
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MDPI AG
2024-02-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/14/3/522 |
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| author | Mingyu Wang Qin Zhong Huailu Xin Bing Yu Jun He Jie Yu Xiangbing Mao Zhiqing Huang Yuheng Luo Junqiu Luo Hui Yan Aimin Wu Junning Pu Ping Zheng |
| author_facet | Mingyu Wang Qin Zhong Huailu Xin Bing Yu Jun He Jie Yu Xiangbing Mao Zhiqing Huang Yuheng Luo Junqiu Luo Hui Yan Aimin Wu Junning Pu Ping Zheng |
| author_sort | Mingyu Wang |
| collection | DOAJ |
| description | Post-weaning diarrhea significantly contributes to the high mortality in pig production, but the metabolic changes in weaned piglets with diarrhea remain unclear. This study aimed to identify the differential metabolites in the urine of diarrheal weaned piglets and those of healthy weaned piglets to reveal the metabolic changes associated with diarrhea in weaned piglets. Nine 25-day-old piglets with diarrhea scores above 16 and an average body weight of 5.41 ± 0.18 kg were selected for the diarrhea group. Corresponding to the body weight and sex of the diarrhea group, nine 25-month-old healthy piglets with similar sex and body weights of 5.49 ± 0.21 kg were selected as the control group. Results showed that the serum C-reactive protein and cortisol of piglets in the diarrhea group were higher than those in the control group (<i>p</i> < 0.05). The mRNA expression of <i>TNF-α</i>, <i>IFN-γ</i> in the jejunum and colon, and <i>IL-1β</i> in the jejunum were increased in diarrhea piglets (<i>p</i> < 0.05), accompanied by a reduction in the mRNA expression of <i>ZO-1</i>, <i>ZO-2</i>, and <i>CLDN1</i> in the jejunum and colon (<i>p</i> < 0.05); mRNA expression of <i>OCLN</i> in the colon also occurred (<i>p</i> < 0.05). Metabolomic analysis of urine revealed increased levels of inosine, hypoxanthine, guanosine, deoxyinosin, glucosamine, glucosamine-1-p, N-Acetylmannosamine, chitobiose, and uric acid, identified as differential metabolites in diarrhea piglets compared to the controls. In summary, elevated weaning stress and inflammatory disease were associated with the abnormalities of purine metabolism and the hexosamine biosynthetic pathway of weaned piglets. This study additionally indicated the presence of energy metabolism-related diseases in diarrheal weaned piglets. |
| first_indexed | 2024-03-08T04:01:31Z |
| format | Article |
| id | doaj.art-564e42e779fb4fc996b86773334209a8 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-08T04:01:31Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-564e42e779fb4fc996b86773334209a82024-02-09T15:07:05ZengMDPI AGAnimals2076-26152024-02-0114352210.3390/ani14030522Purine Metabolism and Hexosamine Biosynthetic Pathway Abnormalities in Diarrheal Weaned Piglets Identified Using MetabolomicsMingyu Wang0Qin Zhong1Huailu Xin2Bing Yu3Jun He4Jie Yu5Xiangbing Mao6Zhiqing Huang7Yuheng Luo8Junqiu Luo9Hui Yan10Aimin Wu11Junning Pu12Ping Zheng13Key Laboratory for Animal Disease-Resistance Nutrition of Sichuan Province, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory for Animal Disease-Resistance Nutrition of Sichuan Province, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory for Animal Disease-Resistance Nutrition of Sichuan Province, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory for Animal Disease-Resistance Nutrition of Sichuan Province, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory for Animal Disease-Resistance Nutrition of Sichuan Province, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory for Animal Disease-Resistance Nutrition of Sichuan Province, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory for Animal Disease-Resistance Nutrition of Sichuan Province, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory for Animal Disease-Resistance Nutrition of Sichuan Province, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory for Animal Disease-Resistance Nutrition of Sichuan Province, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory for Animal Disease-Resistance Nutrition of Sichuan Province, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory for Animal Disease-Resistance Nutrition of Sichuan Province, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory for Animal Disease-Resistance Nutrition of Sichuan Province, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory for Animal Disease-Resistance Nutrition of Sichuan Province, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory for Animal Disease-Resistance Nutrition of Sichuan Province, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, ChinaPost-weaning diarrhea significantly contributes to the high mortality in pig production, but the metabolic changes in weaned piglets with diarrhea remain unclear. This study aimed to identify the differential metabolites in the urine of diarrheal weaned piglets and those of healthy weaned piglets to reveal the metabolic changes associated with diarrhea in weaned piglets. Nine 25-day-old piglets with diarrhea scores above 16 and an average body weight of 5.41 ± 0.18 kg were selected for the diarrhea group. Corresponding to the body weight and sex of the diarrhea group, nine 25-month-old healthy piglets with similar sex and body weights of 5.49 ± 0.21 kg were selected as the control group. Results showed that the serum C-reactive protein and cortisol of piglets in the diarrhea group were higher than those in the control group (<i>p</i> < 0.05). The mRNA expression of <i>TNF-α</i>, <i>IFN-γ</i> in the jejunum and colon, and <i>IL-1β</i> in the jejunum were increased in diarrhea piglets (<i>p</i> < 0.05), accompanied by a reduction in the mRNA expression of <i>ZO-1</i>, <i>ZO-2</i>, and <i>CLDN1</i> in the jejunum and colon (<i>p</i> < 0.05); mRNA expression of <i>OCLN</i> in the colon also occurred (<i>p</i> < 0.05). Metabolomic analysis of urine revealed increased levels of inosine, hypoxanthine, guanosine, deoxyinosin, glucosamine, glucosamine-1-p, N-Acetylmannosamine, chitobiose, and uric acid, identified as differential metabolites in diarrhea piglets compared to the controls. In summary, elevated weaning stress and inflammatory disease were associated with the abnormalities of purine metabolism and the hexosamine biosynthetic pathway of weaned piglets. This study additionally indicated the presence of energy metabolism-related diseases in diarrheal weaned piglets.https://www.mdpi.com/2076-2615/14/3/522post-weaning diarrheametabolomic analysispigletsstress |
| spellingShingle | Mingyu Wang Qin Zhong Huailu Xin Bing Yu Jun He Jie Yu Xiangbing Mao Zhiqing Huang Yuheng Luo Junqiu Luo Hui Yan Aimin Wu Junning Pu Ping Zheng Purine Metabolism and Hexosamine Biosynthetic Pathway Abnormalities in Diarrheal Weaned Piglets Identified Using Metabolomics Animals post-weaning diarrhea metabolomic analysis piglets stress |
| title | Purine Metabolism and Hexosamine Biosynthetic Pathway Abnormalities in Diarrheal Weaned Piglets Identified Using Metabolomics |
| title_full | Purine Metabolism and Hexosamine Biosynthetic Pathway Abnormalities in Diarrheal Weaned Piglets Identified Using Metabolomics |
| title_fullStr | Purine Metabolism and Hexosamine Biosynthetic Pathway Abnormalities in Diarrheal Weaned Piglets Identified Using Metabolomics |
| title_full_unstemmed | Purine Metabolism and Hexosamine Biosynthetic Pathway Abnormalities in Diarrheal Weaned Piglets Identified Using Metabolomics |
| title_short | Purine Metabolism and Hexosamine Biosynthetic Pathway Abnormalities in Diarrheal Weaned Piglets Identified Using Metabolomics |
| title_sort | purine metabolism and hexosamine biosynthetic pathway abnormalities in diarrheal weaned piglets identified using metabolomics |
| topic | post-weaning diarrhea metabolomic analysis piglets stress |
| url | https://www.mdpi.com/2076-2615/14/3/522 |
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