Identification and Validation of the Anoikis-Related Gene Signature as a Novel Prognostic Model for Cervical Squamous Cell Carcinoma, Endocervical Adenocarcinoma, and Revelation Immune Infiltration
<i>Background and Objectives</i>: Cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC) are malignant disorders with adverse prognoses for advanced patients. Anoikis, which is involved in tumor metastasis, facilitates the survival and separation of tumor cells from thei...
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MDPI AG
2023-02-01
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| Series: | Medicina |
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| Online Access: | https://www.mdpi.com/1648-9144/59/2/358 |
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| author | Qin-Qin Jin Jie Mei Lin Hong Rui Wang Shuang-Yue Wu Sen-Lin Wang Xi-Ya Jiang Yin-Ting Yang Hui Yao Wei-Yu Zhang Yu-Ting Zhu Jie Ying Lu Tian Guo Chen Shu-Guang Zhou |
| author_facet | Qin-Qin Jin Jie Mei Lin Hong Rui Wang Shuang-Yue Wu Sen-Lin Wang Xi-Ya Jiang Yin-Ting Yang Hui Yao Wei-Yu Zhang Yu-Ting Zhu Jie Ying Lu Tian Guo Chen Shu-Guang Zhou |
| author_sort | Qin-Qin Jin |
| collection | DOAJ |
| description | <i>Background and Objectives</i>: Cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC) are malignant disorders with adverse prognoses for advanced patients. Anoikis, which is involved in tumor metastasis, facilitates the survival and separation of tumor cells from their initial site. Unfortunately, it is rarely studied, and in the literature, studies have only addressed the prognosis character of anoikis for patients with CESC. <i>Materials and Methods</i>: We utilized anoikis-related genes (ANRGs) to construct a prognostic signature in CESC patients that were selected from the Genecards and Harmonizome portals. Furthermore, we revealed the underlying clinical value of this signature for clinical maneuvers by providing clinical specialists with an innovative nomogram on the basis of ANRGs. Finally, we investigated the immune microenvironment and drug sensitivity in different risk groups. <i>Results</i>: We screened six genes from fifty-eight anoikis-related differentially expressed genes in the TCGA-CESC cohort, and we constructed a prognostic signature. Then, we built a nomogram combined with CESC clinicopathological traits and risk scores, which demonstrated that this model may improve the prognosis of CESC patients in clinical therapy. Next, the prognostic risk scores were confirmed to be an independent prognostic indicator. Additionally, we programmed a series of analyses, which included immune infiltration analysis, therapy-related analysis, and GSVA enrichment analysis, to identify the functions and mechanisms of the prognostic models during the progression of cancer in CESC patients. Finally, we performed quantitative reverse transcription polymerase chain reaction (qRT-PCR) to verify the six ANRGs. <i>Conclusions</i>: The present discovery verified that the predictive 6-anoikis-related gene (6-ANRG) signature and nomogram serve as imperative factors that might notably impact a CESC patient’s prognosis, and they may be able to provide new clinical evidence to assume the role of underlying biological biomarkers and thus become indispensable indicators for prospective diagnoses and advancing therapy. |
| first_indexed | 2024-03-11T08:27:13Z |
| format | Article |
| id | doaj.art-564e959ec22a49bb8fe30cc96490ddf8 |
| institution | Directory Open Access Journal |
| issn | 1010-660X 1648-9144 |
| language | English |
| last_indexed | 2024-03-11T08:27:13Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Medicina |
| spelling | doaj.art-564e959ec22a49bb8fe30cc96490ddf82023-11-16T22:00:41ZengMDPI AGMedicina1010-660X1648-91442023-02-0159235810.3390/medicina59020358Identification and Validation of the Anoikis-Related Gene Signature as a Novel Prognostic Model for Cervical Squamous Cell Carcinoma, Endocervical Adenocarcinoma, and Revelation Immune InfiltrationQin-Qin Jin0Jie Mei1Lin Hong2Rui Wang3Shuang-Yue Wu4Sen-Lin Wang5Xi-Ya Jiang6Yin-Ting Yang7Hui Yao8Wei-Yu Zhang9Yu-Ting Zhu10Jie Ying11Lu Tian12Guo Chen13Shu-Guang Zhou14Department of Gynecology, Maternal and Child Medical Centre of Anhui Medical University, Hefei 230001, ChinaDepartment of Gynecology, Maternal and Child Medical Centre of Anhui Medical University, Hefei 230001, ChinaDepartment of Gynecology, Maternal and Child Medical Centre of Anhui Medical University, Hefei 230001, ChinaOffice of Health Care, Hefei Municipal Health Commission, Hefei 230071, ChinaDepartment of Gynecology, Maternal and Child Medical Centre of Anhui Medical University, Hefei 230001, ChinaDepartment of Clinical Laboratory, Anhui Province Maternity and Child Healthcare Hospital, Hefei 230001, ChinaDepartment of Gynecology, Maternal and Child Medical Centre of Anhui Medical University, Hefei 230001, ChinaDepartment of Gynecology, Maternal and Child Medical Centre of Anhui Medical University, Hefei 230001, ChinaDepartment of Gynecology, Maternal and Child Medical Centre of Anhui Medical University, Hefei 230001, ChinaDepartment of Gynecology, Maternal and Child Medical Centre of Anhui Medical University, Hefei 230001, ChinaDepartment of Gynecology, Maternal and Child Medical Centre of Anhui Medical University, Hefei 230001, ChinaDepartment of Gynecology, Maternal and Child Medical Centre of Anhui Medical University, Hefei 230001, ChinaDepartment of Gynecology, Maternal and Child Medical Centre of Anhui Medical University, Hefei 230001, ChinaDepartment of Gynecology, Maternal and Child Medical Centre of Anhui Medical University, Hefei 230001, ChinaDepartment of Gynecology, Maternal and Child Medical Centre of Anhui Medical University, Hefei 230001, China<i>Background and Objectives</i>: Cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC) are malignant disorders with adverse prognoses for advanced patients. Anoikis, which is involved in tumor metastasis, facilitates the survival and separation of tumor cells from their initial site. Unfortunately, it is rarely studied, and in the literature, studies have only addressed the prognosis character of anoikis for patients with CESC. <i>Materials and Methods</i>: We utilized anoikis-related genes (ANRGs) to construct a prognostic signature in CESC patients that were selected from the Genecards and Harmonizome portals. Furthermore, we revealed the underlying clinical value of this signature for clinical maneuvers by providing clinical specialists with an innovative nomogram on the basis of ANRGs. Finally, we investigated the immune microenvironment and drug sensitivity in different risk groups. <i>Results</i>: We screened six genes from fifty-eight anoikis-related differentially expressed genes in the TCGA-CESC cohort, and we constructed a prognostic signature. Then, we built a nomogram combined with CESC clinicopathological traits and risk scores, which demonstrated that this model may improve the prognosis of CESC patients in clinical therapy. Next, the prognostic risk scores were confirmed to be an independent prognostic indicator. Additionally, we programmed a series of analyses, which included immune infiltration analysis, therapy-related analysis, and GSVA enrichment analysis, to identify the functions and mechanisms of the prognostic models during the progression of cancer in CESC patients. Finally, we performed quantitative reverse transcription polymerase chain reaction (qRT-PCR) to verify the six ANRGs. <i>Conclusions</i>: The present discovery verified that the predictive 6-anoikis-related gene (6-ANRG) signature and nomogram serve as imperative factors that might notably impact a CESC patient’s prognosis, and they may be able to provide new clinical evidence to assume the role of underlying biological biomarkers and thus become indispensable indicators for prospective diagnoses and advancing therapy.https://www.mdpi.com/1648-9144/59/2/358anoikiscervical squamous cell carcinomaendocervical adenocarcinomaprognosistherapeutic target |
| spellingShingle | Qin-Qin Jin Jie Mei Lin Hong Rui Wang Shuang-Yue Wu Sen-Lin Wang Xi-Ya Jiang Yin-Ting Yang Hui Yao Wei-Yu Zhang Yu-Ting Zhu Jie Ying Lu Tian Guo Chen Shu-Guang Zhou Identification and Validation of the Anoikis-Related Gene Signature as a Novel Prognostic Model for Cervical Squamous Cell Carcinoma, Endocervical Adenocarcinoma, and Revelation Immune Infiltration Medicina anoikis cervical squamous cell carcinoma endocervical adenocarcinoma prognosis therapeutic target |
| title | Identification and Validation of the Anoikis-Related Gene Signature as a Novel Prognostic Model for Cervical Squamous Cell Carcinoma, Endocervical Adenocarcinoma, and Revelation Immune Infiltration |
| title_full | Identification and Validation of the Anoikis-Related Gene Signature as a Novel Prognostic Model for Cervical Squamous Cell Carcinoma, Endocervical Adenocarcinoma, and Revelation Immune Infiltration |
| title_fullStr | Identification and Validation of the Anoikis-Related Gene Signature as a Novel Prognostic Model for Cervical Squamous Cell Carcinoma, Endocervical Adenocarcinoma, and Revelation Immune Infiltration |
| title_full_unstemmed | Identification and Validation of the Anoikis-Related Gene Signature as a Novel Prognostic Model for Cervical Squamous Cell Carcinoma, Endocervical Adenocarcinoma, and Revelation Immune Infiltration |
| title_short | Identification and Validation of the Anoikis-Related Gene Signature as a Novel Prognostic Model for Cervical Squamous Cell Carcinoma, Endocervical Adenocarcinoma, and Revelation Immune Infiltration |
| title_sort | identification and validation of the anoikis related gene signature as a novel prognostic model for cervical squamous cell carcinoma endocervical adenocarcinoma and revelation immune infiltration |
| topic | anoikis cervical squamous cell carcinoma endocervical adenocarcinoma prognosis therapeutic target |
| url | https://www.mdpi.com/1648-9144/59/2/358 |
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