Cloning of miR319b precursor gene and expressing analysis during the in vitro plantlets development by qPCR in Amaranthus tricolor L(苋菜试管苗ama-miR319b前体的克隆及其在苋菜试管苗发育过程中的表达分析)
试验以苋菜试管苗及该试管苗发育过程中各阶段培养物为材料，进行miR319b前体的克隆及其在苋菜试管苗发育过程中的qPCR分析.结果表明：采用RT-PCR法克隆到苋菜miR319b前体序列，长186 bp.该前体与烟草、苜蓿等miR319b前体序列具有较高的同源性，并包含长21 bp的miR319b的成熟序列；利用qPCR技术研究显示，该miRNA在苋菜试管苗发育过程中的表达具有特异性，在幼苗期时相对表达量最低，而在壮苗期和花芽期相对表达量迅速增加，且在花芽期时达到最高值；进入开花期，其相对表达量又呈下降趋势，但略高于壮苗期.说明ama-miR319b前体在苋菜幼苗期向壮苗期转变及花芽期向开花期...
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| Format: | Article |
| Language: | zho |
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Zhejiang University Press
2014-07-01
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| Series: | Zhejiang Daxue xuebao. Lixue ban |
| Subjects: | |
| Online Access: | https://doi.org/10.3785/j.issn.1008-9497.2014.04.015 |
| _version_ | 1827303054778564608 |
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| author | LIUShengcai(刘生财) KUANGHuaqin(匡华琴) XIELiyang(谢礼洋) LAIZhongxiong(赖钟雄) |
| author_facet | LIUShengcai(刘生财) KUANGHuaqin(匡华琴) XIELiyang(谢礼洋) LAIZhongxiong(赖钟雄) |
| author_sort | LIUShengcai(刘生财) |
| collection | DOAJ |
| description | 试验以苋菜试管苗及该试管苗发育过程中各阶段培养物为材料，进行miR319b前体的克隆及其在苋菜试管苗发育过程中的qPCR分析.结果表明：采用RT-PCR法克隆到苋菜miR319b前体序列，长186 bp.该前体与烟草、苜蓿等miR319b前体序列具有较高的同源性，并包含长21 bp的miR319b的成熟序列；利用qPCR技术研究显示，该miRNA在苋菜试管苗发育过程中的表达具有特异性，在幼苗期时相对表达量最低，而在壮苗期和花芽期相对表达量迅速增加，且在花芽期时达到最高值；进入开花期，其相对表达量又呈下降趋势，但略高于壮苗期.说明ama-miR319b前体在苋菜幼苗期向壮苗期转变及花芽期向开花期转变阶段可能起重要作用，这为将来进一步研究其功能奠定了基础. |
| first_indexed | 2024-04-24T16:55:29Z |
| format | Article |
| id | doaj.art-564f45d018d5430e9434dc3580352376 |
| institution | Directory Open Access Journal |
| issn | 1008-9497 |
| language | zho |
| last_indexed | 2024-04-24T16:55:29Z |
| publishDate | 2014-07-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | Zhejiang Daxue xuebao. Lixue ban |
| spelling | doaj.art-564f45d018d5430e9434dc35803523762024-03-29T01:58:33ZzhoZhejiang University PressZhejiang Daxue xuebao. Lixue ban1008-94972014-07-0141443543910.3785/j.issn.1008-9497.2014.04.015Cloning of miR319b precursor gene and expressing analysis during the in vitro plantlets development by qPCR in Amaranthus tricolor L(苋菜试管苗ama-miR319b前体的克隆及其在苋菜试管苗发育过程中的表达分析)LIUShengcai(刘生财)0KUANGHuaqin(匡华琴)1XIELiyang(谢礼洋)2LAIZhongxiong(赖钟雄)3Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China(福建农林大学园艺植物生物工程研究所，福建 福州 350002)Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China(福建农林大学园艺植物生物工程研究所，福建 福州 350002)Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China(福建农林大学园艺植物生物工程研究所，福建 福州 350002)Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China(福建农林大学园艺植物生物工程研究所，福建 福州 350002)试验以苋菜试管苗及该试管苗发育过程中各阶段培养物为材料，进行miR319b前体的克隆及其在苋菜试管苗发育过程中的qPCR分析.结果表明：采用RT-PCR法克隆到苋菜miR319b前体序列，长186 bp.该前体与烟草、苜蓿等miR319b前体序列具有较高的同源性，并包含长21 bp的miR319b的成熟序列；利用qPCR技术研究显示，该miRNA在苋菜试管苗发育过程中的表达具有特异性，在幼苗期时相对表达量最低，而在壮苗期和花芽期相对表达量迅速增加，且在花芽期时达到最高值；进入开花期，其相对表达量又呈下降趋势，但略高于壮苗期.说明ama-miR319b前体在苋菜幼苗期向壮苗期转变及花芽期向开花期转变阶段可能起重要作用，这为将来进一步研究其功能奠定了基础.https://doi.org/10.3785/j.issn.1008-9497.2014.04.015苋菜试管苗发育mir319b前体克隆qpcr |
| spellingShingle | LIUShengcai(刘生财) KUANGHuaqin(匡华琴) XIELiyang(谢礼洋) LAIZhongxiong(赖钟雄) Cloning of miR319b precursor gene and expressing analysis during the in vitro plantlets development by qPCR in Amaranthus tricolor L(苋菜试管苗ama-miR319b前体的克隆及其在苋菜试管苗发育过程中的表达分析) Zhejiang Daxue xuebao. Lixue ban 苋菜 试管苗发育 mir319b前体 克隆 qpcr |
| title | Cloning of miR319b precursor gene and expressing analysis during the in vitro plantlets development by qPCR in Amaranthus tricolor L(苋菜试管苗ama-miR319b前体的克隆及其在苋菜试管苗发育过程中的表达分析) |
| title_full | Cloning of miR319b precursor gene and expressing analysis during the in vitro plantlets development by qPCR in Amaranthus tricolor L(苋菜试管苗ama-miR319b前体的克隆及其在苋菜试管苗发育过程中的表达分析) |
| title_fullStr | Cloning of miR319b precursor gene and expressing analysis during the in vitro plantlets development by qPCR in Amaranthus tricolor L(苋菜试管苗ama-miR319b前体的克隆及其在苋菜试管苗发育过程中的表达分析) |
| title_full_unstemmed | Cloning of miR319b precursor gene and expressing analysis during the in vitro plantlets development by qPCR in Amaranthus tricolor L(苋菜试管苗ama-miR319b前体的克隆及其在苋菜试管苗发育过程中的表达分析) |
| title_short | Cloning of miR319b precursor gene and expressing analysis during the in vitro plantlets development by qPCR in Amaranthus tricolor L(苋菜试管苗ama-miR319b前体的克隆及其在苋菜试管苗发育过程中的表达分析) |
| title_sort | cloning of mir319b precursor gene and expressing analysis during the in vitro plantlets development by qpcr in amaranthus tricolor l 苋菜试管苗ama mir319b前体的克隆及其在苋菜试管苗发育过程中的表达分析 |
| topic | 苋菜 试管苗发育 mir319b前体 克隆 qpcr |
| url | https://doi.org/10.3785/j.issn.1008-9497.2014.04.015 |
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