The value of desmosomal plaque-related markers to distinguish squamous cell carcinoma and adenocarcinoma of the lung
Background: An antibody panel is needed to definitively differentiate between adenocarcinoma (AC) and squamous cell carcinoma (SCC) in order to meet more stringent requirements for the histologic classification of lung cancers. Staining of desmosomal plaque-related proteins may be useful in the diag...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Upsala Medical Society
2020-01-01
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| Series: | Upsala Journal of Medical Sciences |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/03009734.2019.1692101 |
| _version_ | 1797699923328630784 |
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| author | Inmaculada Galindo Mercedes Gómez-Morales Inés Díaz-Cano Álvaro Andrades Mercedes Caba-Molina María Teresa Miranda-León Pedro Pablo Medina Joel Martín-Padron María Esther Fárez-Vidal |
| author_facet | Inmaculada Galindo Mercedes Gómez-Morales Inés Díaz-Cano Álvaro Andrades Mercedes Caba-Molina María Teresa Miranda-León Pedro Pablo Medina Joel Martín-Padron María Esther Fárez-Vidal |
| author_sort | Inmaculada Galindo |
| collection | DOAJ |
| description | Background: An antibody panel is needed to definitively differentiate between adenocarcinoma (AC) and squamous cell carcinoma (SCC) in order to meet more stringent requirements for the histologic classification of lung cancers. Staining of desmosomal plaque-related proteins may be useful in the diagnosis of lung SCC. Materials and methods: We compared the usefulness of six conventional (CK5/6, p40, p63, CK7, TTF1, and Napsin A) and three novel (PKP1, KRT15, and DSG3) markers to distinguish between lung SCC and AC in 85 small biopsy specimens (41 ACs and 44 SCCs). Correlations were examined between expression of the markers and patients’ histologic and clinical data. Results: The specificity for SCC of membrane staining for PKP1, KRT15, and DSG3 was 97.4%, 94.6%, and 100%, respectively, and it was 100% when the markers were used together and in combination with the conventional markers (AUCs of 0.7619 for Panel 1 SCC, 0.7375 for Panel 2 SCC, 0.8552 for Panel 1 AC, and 0.8088 for Panel 2 AC). In a stepwise multivariate logistic regression model, the combination of CK5/6, p63, and PKP1 in membrane was the optimal panel to differentiate between SCC and AC, with a percentage correct classification of 96.2% overall (94.6% of ACs and 97.6% of SCCs). PKP1 and DSG3 are related to the prognosis. Conclusions: PKP1, KRT15, and DSG3 are highly specific for SCC, but they were more useful to differentiate between SCC and AC when used together and in combination with conventional markers. PKP1 and DSG3 expressions may have prognostic value. |
| first_indexed | 2024-03-12T04:15:59Z |
| format | Article |
| id | doaj.art-564ff5467a934a60977a380aef40854b |
| institution | Directory Open Access Journal |
| issn | 0300-9734 2000-1967 |
| language | English |
| last_indexed | 2024-03-12T04:15:59Z |
| publishDate | 2020-01-01 |
| publisher | Upsala Medical Society |
| record_format | Article |
| series | Upsala Journal of Medical Sciences |
| spelling | doaj.art-564ff5467a934a60977a380aef40854b2023-09-03T10:37:44ZengUpsala Medical SocietyUpsala Journal of Medical Sciences0300-97342000-19672020-01-011251192910.1080/03009734.2019.16921011692101The value of desmosomal plaque-related markers to distinguish squamous cell carcinoma and adenocarcinoma of the lungInmaculada Galindo0Mercedes Gómez-Morales1Inés Díaz-Cano2Álvaro Andrades3Mercedes Caba-Molina4María Teresa Miranda-León5Pedro Pablo Medina6Joel Martín-Padron7María Esther Fárez-Vidal8University of GranadaUniversity of GranadaUniversity of GranadaCentre for Genomics and Oncological Research (GENYO)University of GranadaUniversity of GranadaCentre for Genomics and Oncological Research (GENYO)University of GranadaUniversity of GranadaBackground: An antibody panel is needed to definitively differentiate between adenocarcinoma (AC) and squamous cell carcinoma (SCC) in order to meet more stringent requirements for the histologic classification of lung cancers. Staining of desmosomal plaque-related proteins may be useful in the diagnosis of lung SCC. Materials and methods: We compared the usefulness of six conventional (CK5/6, p40, p63, CK7, TTF1, and Napsin A) and three novel (PKP1, KRT15, and DSG3) markers to distinguish between lung SCC and AC in 85 small biopsy specimens (41 ACs and 44 SCCs). Correlations were examined between expression of the markers and patients’ histologic and clinical data. Results: The specificity for SCC of membrane staining for PKP1, KRT15, and DSG3 was 97.4%, 94.6%, and 100%, respectively, and it was 100% when the markers were used together and in combination with the conventional markers (AUCs of 0.7619 for Panel 1 SCC, 0.7375 for Panel 2 SCC, 0.8552 for Panel 1 AC, and 0.8088 for Panel 2 AC). In a stepwise multivariate logistic regression model, the combination of CK5/6, p63, and PKP1 in membrane was the optimal panel to differentiate between SCC and AC, with a percentage correct classification of 96.2% overall (94.6% of ACs and 97.6% of SCCs). PKP1 and DSG3 are related to the prognosis. Conclusions: PKP1, KRT15, and DSG3 are highly specific for SCC, but they were more useful to differentiate between SCC and AC when used together and in combination with conventional markers. PKP1 and DSG3 expressions may have prognostic value.http://dx.doi.org/10.1080/03009734.2019.1692101adenocarcinomadesmosomal plaque proteinsnon-small-cell lung cancersquamous cell carcinoma |
| spellingShingle | Inmaculada Galindo Mercedes Gómez-Morales Inés Díaz-Cano Álvaro Andrades Mercedes Caba-Molina María Teresa Miranda-León Pedro Pablo Medina Joel Martín-Padron María Esther Fárez-Vidal The value of desmosomal plaque-related markers to distinguish squamous cell carcinoma and adenocarcinoma of the lung Upsala Journal of Medical Sciences adenocarcinoma desmosomal plaque proteins non-small-cell lung cancer squamous cell carcinoma |
| title | The value of desmosomal plaque-related markers to distinguish squamous cell carcinoma and adenocarcinoma of the lung |
| title_full | The value of desmosomal plaque-related markers to distinguish squamous cell carcinoma and adenocarcinoma of the lung |
| title_fullStr | The value of desmosomal plaque-related markers to distinguish squamous cell carcinoma and adenocarcinoma of the lung |
| title_full_unstemmed | The value of desmosomal plaque-related markers to distinguish squamous cell carcinoma and adenocarcinoma of the lung |
| title_short | The value of desmosomal plaque-related markers to distinguish squamous cell carcinoma and adenocarcinoma of the lung |
| title_sort | value of desmosomal plaque related markers to distinguish squamous cell carcinoma and adenocarcinoma of the lung |
| topic | adenocarcinoma desmosomal plaque proteins non-small-cell lung cancer squamous cell carcinoma |
| url | http://dx.doi.org/10.1080/03009734.2019.1692101 |
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