Selective Biological Effects of Selenium-Enriched Polysaccharide (Se-Le-30) Isolated from <i>Lentinula edodes</i> Mycelium on Human Immune Cells
A common edible mushroom <i>Lentinula edodes,</i> is an important source of numerous biologically active substances, including polysaccharides, with immunomodulatory and antitumor properties. In the present work, the biological activity of the crude, homogenous (Se)-enriched fraction (na...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-11-01
|
| Series: | Biomolecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-273X/11/12/1777 |
| _version_ | 1827673938512052224 |
|---|---|
| author | Beata Kaleta Aleksander Roszczyk Michał Zych Monika Kniotek Radosław Zagożdżon Marzenna Klimaszewska Eliza Malinowska Michał Pac Jadwiga Turło |
| author_facet | Beata Kaleta Aleksander Roszczyk Michał Zych Monika Kniotek Radosław Zagożdżon Marzenna Klimaszewska Eliza Malinowska Michał Pac Jadwiga Turło |
| author_sort | Beata Kaleta |
| collection | DOAJ |
| description | A common edible mushroom <i>Lentinula edodes,</i> is an important source of numerous biologically active substances, including polysaccharides, with immunomodulatory and antitumor properties. In the present work, the biological activity of the crude, homogenous (Se)-enriched fraction (named Se-Le-30), which has been isolated from <i>L. edodes</i> mycelium by a modified Chihara method towards human peripheral blood mononuclear cells (PBMCs) and peripheral granulocytes, was investigated. The Se-Le-30 fraction, an analog of lentinan, significantly inhibited the proliferation of human PBMCs stimulated with anti-CD3 antibodies or allostimulated, and down-regulated the production of tumor necrosis factor (TNF)-α by CD3+ T cells. Moreover, it was found that Se-Le-30 significantly reduced the cytotoxic activity of human natural killer (NK) cells. The results suggested the selective immunosuppressive activity of this fraction, which is non-typical for mushroom derived polysaccharides. |
| first_indexed | 2024-03-10T04:32:43Z |
| format | Article |
| id | doaj.art-56523e2cb82a450187e737ec40c2d37f |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T04:32:43Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-56523e2cb82a450187e737ec40c2d37f2023-11-23T03:58:57ZengMDPI AGBiomolecules2218-273X2021-11-011112177710.3390/biom11121777Selective Biological Effects of Selenium-Enriched Polysaccharide (Se-Le-30) Isolated from <i>Lentinula edodes</i> Mycelium on Human Immune CellsBeata Kaleta0Aleksander Roszczyk1Michał Zych2Monika Kniotek3Radosław Zagożdżon4Marzenna Klimaszewska5Eliza Malinowska6Michał Pac7Jadwiga Turło8Department of Clinical Immunology, Medical University of Warsaw, Nowogrodzka 59, 02-006 Warsaw, PolandDepartment of Clinical Immunology, Medical University of Warsaw, Nowogrodzka 59, 02-006 Warsaw, PolandDepartment of Clinical Immunology, Medical University of Warsaw, Nowogrodzka 59, 02-006 Warsaw, PolandDepartment of Clinical Immunology, Medical University of Warsaw, Nowogrodzka 59, 02-006 Warsaw, PolandDepartment of Clinical Immunology, Medical University of Warsaw, Nowogrodzka 59, 02-006 Warsaw, PolandDepartment of Drug Technology and Pharmaceutical Biotechnology, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, PolandDepartment of Drug Technology and Pharmaceutical Biotechnology, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, PolandDepartment of Immunology, Transplantology, and Internal Diseases, Medical University of Warsaw, Nowogrodzka 59, 02-006 Warsaw, PolandDepartment of Drug Technology and Pharmaceutical Biotechnology, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, PolandA common edible mushroom <i>Lentinula edodes,</i> is an important source of numerous biologically active substances, including polysaccharides, with immunomodulatory and antitumor properties. In the present work, the biological activity of the crude, homogenous (Se)-enriched fraction (named Se-Le-30), which has been isolated from <i>L. edodes</i> mycelium by a modified Chihara method towards human peripheral blood mononuclear cells (PBMCs) and peripheral granulocytes, was investigated. The Se-Le-30 fraction, an analog of lentinan, significantly inhibited the proliferation of human PBMCs stimulated with anti-CD3 antibodies or allostimulated, and down-regulated the production of tumor necrosis factor (TNF)-α by CD3+ T cells. Moreover, it was found that Se-Le-30 significantly reduced the cytotoxic activity of human natural killer (NK) cells. The results suggested the selective immunosuppressive activity of this fraction, which is non-typical for mushroom derived polysaccharides.https://www.mdpi.com/2218-273X/11/12/1777<i>Lentinula edodes</i>seleniumpolysaccharideimmunosuppressant |
| spellingShingle | Beata Kaleta Aleksander Roszczyk Michał Zych Monika Kniotek Radosław Zagożdżon Marzenna Klimaszewska Eliza Malinowska Michał Pac Jadwiga Turło Selective Biological Effects of Selenium-Enriched Polysaccharide (Se-Le-30) Isolated from <i>Lentinula edodes</i> Mycelium on Human Immune Cells Biomolecules <i>Lentinula edodes</i> selenium polysaccharide immunosuppressant |
| title | Selective Biological Effects of Selenium-Enriched Polysaccharide (Se-Le-30) Isolated from <i>Lentinula edodes</i> Mycelium on Human Immune Cells |
| title_full | Selective Biological Effects of Selenium-Enriched Polysaccharide (Se-Le-30) Isolated from <i>Lentinula edodes</i> Mycelium on Human Immune Cells |
| title_fullStr | Selective Biological Effects of Selenium-Enriched Polysaccharide (Se-Le-30) Isolated from <i>Lentinula edodes</i> Mycelium on Human Immune Cells |
| title_full_unstemmed | Selective Biological Effects of Selenium-Enriched Polysaccharide (Se-Le-30) Isolated from <i>Lentinula edodes</i> Mycelium on Human Immune Cells |
| title_short | Selective Biological Effects of Selenium-Enriched Polysaccharide (Se-Le-30) Isolated from <i>Lentinula edodes</i> Mycelium on Human Immune Cells |
| title_sort | selective biological effects of selenium enriched polysaccharide se le 30 isolated from i lentinula edodes i mycelium on human immune cells |
| topic | <i>Lentinula edodes</i> selenium polysaccharide immunosuppressant |
| url | https://www.mdpi.com/2218-273X/11/12/1777 |
| work_keys_str_mv | AT beatakaleta selectivebiologicaleffectsofseleniumenrichedpolysaccharidesele30isolatedfromilentinulaedodesimyceliumonhumanimmunecells AT aleksanderroszczyk selectivebiologicaleffectsofseleniumenrichedpolysaccharidesele30isolatedfromilentinulaedodesimyceliumonhumanimmunecells AT michałzych selectivebiologicaleffectsofseleniumenrichedpolysaccharidesele30isolatedfromilentinulaedodesimyceliumonhumanimmunecells AT monikakniotek selectivebiologicaleffectsofseleniumenrichedpolysaccharidesele30isolatedfromilentinulaedodesimyceliumonhumanimmunecells AT radosławzagozdzon selectivebiologicaleffectsofseleniumenrichedpolysaccharidesele30isolatedfromilentinulaedodesimyceliumonhumanimmunecells AT marzennaklimaszewska selectivebiologicaleffectsofseleniumenrichedpolysaccharidesele30isolatedfromilentinulaedodesimyceliumonhumanimmunecells AT elizamalinowska selectivebiologicaleffectsofseleniumenrichedpolysaccharidesele30isolatedfromilentinulaedodesimyceliumonhumanimmunecells AT michałpac selectivebiologicaleffectsofseleniumenrichedpolysaccharidesele30isolatedfromilentinulaedodesimyceliumonhumanimmunecells AT jadwigaturło selectivebiologicaleffectsofseleniumenrichedpolysaccharidesele30isolatedfromilentinulaedodesimyceliumonhumanimmunecells |